Chitosan semi-fluid slow-release gel and its application

A slow-release gel and chitosan technology, applied in prosthetics, liquid delivery, medical science, etc., can solve the problem of easy inactivation of water-soluble protein drugs and achieve good biocompatibility

Inactive Publication Date: 2016-10-05
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there are also problems. Due to the presence of concentrated acid, concentrated alkali and organic reagents in the production process, water-soluble protein drugs are easily inactivated during the preparation process.

Method used

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  • Chitosan semi-fluid slow-release gel and its application
  • Chitosan semi-fluid slow-release gel and its application
  • Chitosan semi-fluid slow-release gel and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A chitosan semi-fluid slow-release gel is made from the following components:

[0023] Component 1: a chitosan solution with a concentration of 0.5020g / ml prepared with physiological saline as a solvent, the molecular weight of chitosan is 5kDa, the degree of deacetylation is 95%, and it is obtained by dissolving at 4°C;

[0024] Component 2: a hyaluronic acid chitosan solution with a concentration of 0.2510 g / ml was prepared by using a mixture of saturated sodium bicarbonate aqueous solution and distilled water with a volume ratio of 1:1 as a solvent, and the hyaluronic acid chitosan solution was The molecular weight is 500kDa, the intrinsic viscosity is 3000g / ml, and it is obtained by dissolving at 4℃.

[0025] Before use, mix component 1 and component 2 in a 1:1 volume ratio at room temperature to form a semi-fluid gel, see figure 1 A, available for injection. The mixture was heated to 37 °C for 3 hours, and it was in a solidified state, see figure 1 B.

[0026] ...

Embodiment 2

[0030] A chitosan semi-fluid slow-release gel is made from the following components:

[0031] Component 1: a chitosan solution with a concentration of 0.5020g / ml prepared with distilled water as a solvent, the molecular weight of chitosan is 5kDa, the degree of deacetylation is 95%, and it is obtained by dissolving at 4°C;

[0032] Component 2: a hyaluronic acid chitosan solution with a concentration of 0.2510 g / ml was prepared by using a mixture of saturated sodium bicarbonate aqueous solution and distilled water with a volume ratio of 1:1 as a solvent, and the hyaluronic acid chitosan solution was The molecular weight is 500kDa, the intrinsic viscosity is 3000g / ml, and it is obtained by dissolving at 4℃.

[0033] Component 1 and Component 2 are mixed in a volume ratio of 1:1 at room temperature before use to form a semi-fluid gel, ready for injection.

[0034] The method for fluorescent labeling of bovine serum albumin is to dissolve 20 mg of protein in 5 mL of carbonate bu...

Embodiment 3

[0038]A chitosan semi-fluid slow-release gel is made from the following components:

[0039] Component 1: a chitosan solution with a concentration of 0.5020g / ml prepared with physiological saline as a solvent, the molecular weight of chitosan is 3kDa, the degree of deacetylation is 65%, and it is obtained by dissolving at 4°C;

[0040] Component 2: The mixture of saturated sodium bicarbonate aqueous solution and distilled water with a volume ratio of 1:1 is used as a solvent to prepare a hyaluronic acid chitosan solution with a concentration of 0.2510g / ml. The molecular weight of hyaluronic acid chitosan It is 500kDa, and the intrinsic viscosity is 3000g / ml obtained by dissolving at 4°C.

[0041] Before use, mix component 1 and component 2 in a volume ratio of 1:1 at room temperature to form an injectable semi-fluid state.

[0042] Experiments have shown that lipid-soluble drugs with properties similar to paclitaxel: rapamycin, methotrexate, fluorouracil, mercaptopurine, hydr...

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Abstract

The invention discloses a chitosan semi-fluid slow release gel. The chitosan semi-fluid slow release gel comprises an ingredient I and an ingredient II, wherein the ingredient I is obtained by dissolving 0.5020g / ml of prepared chitosan solution at the temperature of 4 DEG C with normal saline or distilled water as a solvent, and the ingredient II is obtained by dissolving 0.251g / ml of prepared hyaluronic acid chitosan solution at the temperature of 4 DEG C with mixed liquid formed by saturated sodium bicarbonate water solution and distilled water at the volume ratio of 1:1 as a solvent. The chitosan semi-fluid slow release gel is prepared by the steps that the ingredient I and the ingredient II are proportionally mixed at the normal temperature before usage to form a mixture in an injectable semi-fluid state, the injected position gradually solidifies and is finally cured when the temperature rises in a range from 26 DEG C to 40 DEG C, and slow drug release can be controlled. A semi-fluid gel preparation prepared by the gel can serve as a drug carrier and a scaffold for tissue engineering. The chitosan semi-fluid slow release gel is biodegradable and good in biocompatibility.

Description

technical field [0001] The invention belongs to a chitosan semi-fluid preparation, in particular to a chitosan semi-fluid gel and its application. Background technique [0002] Chitosan semi-fluid sustained-release gel is a semi-fluid at room temperature and solid at 37°C or in vivo, a temperature-responsive controlled-release preparation. One of its major advantages is its low fluidity, which avoids the loss of the carried drug during fluid flow, and when injected into a human patient, it can well wrap the drug for sustained release (Emily Mastronardi, Amand aFoster, Xueru Zhang, Maria C. DeRosa. Smart Materials Based on DNA Aptamers: Taking Aptasensing to the Next Level. Sensors, 2014;14:3156-3171.). Such properties are similar to temperature-sensitive hydrogels, i.e., easy drug encapsulation, drug delivery, good biocompatibility, and degradability. The main sources of materials are natural and synthetic materials. Chitosan is also called chitosan. Chitosan, as a natural...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K9/06A61L27/20A61L27/52
Inventor 俞玫夏婷孙洪范孔德领李书仪
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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