Fingerprint detection method and standard fingerprint of Zizhu nudiflora medicinal materials, intermediates and their preparations

A naked flower purple bead, fingerprint technology, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problems such as the inability to better guarantee the clinical efficacy, effective control of product quality, and inability to use

Active Publication Date: 2016-12-14
JIUZHITANG +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no good quality control method in the prior art to accurately reflect the quality status of existing Chinese medicinal materials, intermediates and finished products, and it cannot be used in the production process and the effective control of product quality, so it cannot be better guaranteed Its clinical efficacy

Method used

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  • Fingerprint detection method and standard fingerprint of Zizhu nudiflora medicinal materials, intermediates and their preparations
  • Fingerprint detection method and standard fingerprint of Zizhu nudiflora medicinal materials, intermediates and their preparations
  • Fingerprint detection method and standard fingerprint of Zizhu nudiflora medicinal materials, intermediates and their preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Establishment of fingerprints of the medicinal material of Auranthus nudiflora.

[0061] (1) Source of medicinal materials

[0062] In order to make the medicinal materials sufficiently representative, medicinal materials from different origins or different harvest seasons were collected as test samples. See Table 1.

[0063] Table 1 Sources of medicinal materials of Zizhu nudiflora

[0064]

[0065] (2) Instruments and reagents

[0066] Agilent 1200 HPLC, ChemStation chromatography workstation; column: ZORBAX SB C 18 Column (4.6mm×250mm, 5µm, Agilent, USA); KQ 3200 DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0067] (3) Selection of chromatographic conditions

[0068] ①Selection of mobile phase

[0069] Select acetonitrile-water, acetonitrile-0.1% phosphoric acid solution, acetonitrile-0.4% phosphoric acid solution, acetonitril...

Embodiment 2

[0102] Example 2 Establishment of the fingerprint of the intermediate of Auranthus nudiflora.

[0103] (1) Instruments and reagents

[0104] Agilent 1200 HPLC, ChemStation chromatography workstation; column: ZORBAX SB C 18 Column (4.6mm×250mm, 5µm, Agilent, USA); KQ 3200 DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0105] (2) Chromatographic conditions

[0106] The chromatographic column is a C18 reverse-phase chromatographic column; the column temperature is 30°C; the detection wavelength is 327nm; the mobile phase A is acetonitrile, the mobile phase B is 0.4% phosphoric acid solution, and the total flow rate is 1.0ml / min; the analysis time is 25 minutes;

[0107] Table 4 Gradient running table

[0108]

[0109] (3) Preparation of reference solution

[0110] Weigh the reference substance of isoverbacoside, place it in a volumetric flask, add 95% ...

Embodiment 3

[0127] Example 3 Establishment of fingerprints of the Nudehuazizhu preparation (taking Nudehuazizhu Tablets as an example).

[0128] (1) Instruments and reagents

[0129] Agilent 1200 high performance liquid chromatography, ChemStation chromatographic workstation; Chromatographic column: ZORBAX SB C18 column (4.6mm × 250mm, 5µm, American Agilent Company); KQ 3200 DE type numerical control ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli- Q pure water system (Millipore, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0130] (2) Chromatographic conditions

[0131] The chromatographic column is a C18 reverse-phase chromatographic column; the column temperature is 30°C; the detection wavelength is 327nm; the mobile phase A is acetonitrile, the mobile phase B is 0.4% phosphoric acid solution, and the total flow rate is 1.0ml / min; the analysis time is 25 minutes;

[0132] Table 6 Gradient running table

[0133]

[0134] (3) Preparation of refer...

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Abstract

The invention discloses a method for detecting the fingerprints of purple bead nudiflora medicinal materials, intermediates and preparations thereof. The method includes: (a) preparation of reference substance solution; (b) preparation of test sample solution; (c) chromatographic conditions; (d) establishment of standard fingerprint with isoverbacoside as the reference peak; (e) fingerprint quality control. The method is simple in the pretreatment method of each test sample, the characteristic components are kept intact, the repeatability and stability are good, the precision is high, and it has certain specificity; the separation effect of each characteristic peak in the obtained fingerprint is good, and the naked flower The fingerprints of Zizhu medicinal materials, intermediates and preparations have a good correlation; it can be used to identify fake and inferior medicinal materials of Zizhu nudiflora, increase the controllability of the production process of Zizhu nudiflora preparations, and help ensure the production of Zizhu nudiflora preparations stable quality and clinical efficacy. The invention also discloses the standard fingerprints of the Auranthus nudiflora medicinal material, intermediates and preparations thereof obtained according to the fingerprint detection method.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for detecting fingerprints of Auranthus nudiflora, intermediates and preparations thereof, and obtains standard fingerprints thereof. Background technique [0002] Callicarpa nudiflora Hook.et Arn. is the dry leaf of Verbenaceae plant. Distributed in Guangdong, Guangxi, Hainan and other places, it grows at an altitude of 1200 meters. The main components of Nadihua Zizhu are flavonoid glycosides, condensed tannins, neutral resins, phenols, polysaccharides, hydroxyl compounds, magnesium, calcium, and iron salts. Modern studies have shown that its active ingredients have hemostatic, antibacterial, astringent, detoxifying, analgesic, and healing-promoting effects. Its antibacterial and antiviral effects, especially have different inhibitory effects on Staphylococcus aureus, typhoid bacillus, and pneumococcus, among which the antibacterial effect on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 卢凤来张妮瑜谷陟欣辛秀李典鹏袁莉黄胜颜冬兰朱丽颜小捷陈诗圣
Owner JIUZHITANG
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