Gadolinium-doped manganese carbonate dual-mode imaging probe for brain glioma

A manganese carbonate dual imaging probe technology, which is applied in the direction of genetic material components, preparations for in vivo experiments, nuclear magnetic resonance/magnetic resonance imaging contrast agents, etc. Solve the problem of low solubility, to solve the effect of water solubility

Inactive Publication Date: 2015-01-07
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is because the particle size used in the brain usually needs to be less than 100 nanometers, and the current manganese carbonate is mostly in the micron range, which is far from meeting the needs of the brain application.
[0007] On the other hand, the solubility of manganese carbonate in water is very low and unstable, making it difficult for biological application

Method used

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  • Gadolinium-doped manganese carbonate dual-mode imaging probe for brain glioma
  • Gadolinium-doped manganese carbonate dual-mode imaging probe for brain glioma
  • Gadolinium-doped manganese carbonate dual-mode imaging probe for brain glioma

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Experimental program
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Embodiment 1

[0040] MnCO 3 Preparation of -OA:

[0041] 1) Preparation of gadolinium oleate: 20mmol GdCl 3 , 60mmol sodium oleate, 30mL distilled water, 40mL ethanol, and 70mL n-hexane were added to the three-necked flask at the same time, wherein GdCl 3 The molar ratio with sodium oleate is 1:3, react at 70°C for 4 hours. Cool to room temperature, let stand to separate the layers, and wash the organic layer three times with distilled water. Dry to obtain gadolinium oleate.

[0042] 2) Preparation of manganese oleate: 20mmol MnCl 2 , 40mmol sodium oleate, 30mL distilled water, 40mL ethanol, and 70mL n-hexane were added to the three-necked flask at the same time, where MnCl2 The molar ratio with sodium oleate is 1:2, react at 70°C for 4 hours. Cool to room temperature, let stand for liquid separation, wash the organic phase three times with distilled water, and dry to obtain manganese oleate.

[0043] 3) Preparation of gadolinium-doped manganese carbonate coated with oleic acid: Disso...

Embodiment 2

[0046] Preparation of silane carboxylic acid modified gadolinium doped manganese carbonate:

[0047] Preparation of water-dispersed gadolinium-doped manganese carbonate nanoparticles by ligand exchange method: Dissolve 100 mg of oleic acid-coated gadolinium-doped manganese carbonate nanoparticles in 60 mL of anhydrous toluene, add 60 μL of acetic acid, sonicate for 15 minutes, and add 0.6 mL of silane Carboxylic acid, react at 70°C for 48 hours. The obtained silane carboxylic acid-modified gadolinium-doped manganese carbonate nanoparticles were washed three times with toluene and anhydrous methanol respectively, dialyzed for 24 hours, and freeze-dried.

Embodiment 3

[0049] Preparation of gadolinium-doped manganese carbonate dual-mode imaging probe:

[0050] 1. Dissolve 100mg of silanized gadolinium-doped manganese carbonate nanoparticles, 3.206mg of EDC, 4.808mg of NHS in PBS, activate at pH 6 for half an hour, then add 20mg of H 2 N-PEG 2240 -FA, 5.055 mg H 2 N-PEG 2000 -Cy5.5, pH 8, reacted for 24 hours, purified by dialysis and then freeze-dried to obtain a gadolinium-doped manganese carbonate dual-mode imaging probe linked to a specific folic acid target.

[0051] 2. Dissolve 100mg of silanized gadolinium-doped manganese carbonate nanoparticles, 3.206mg of EDC, 4.808mg of NHS in PBS, activate at pH 6 for half an hour, then add 17.86mg of H 2 N-PEG 2000 -COOH, 5.055 mg H 2 N-PEG 2000 -Cy5.5, pH 8, reacted for 24 hours, purified by dialysis and freeze-dried to obtain a non-specific gadolinium-doped manganese carbonate dual-mode imaging probe.

[0052] The product Cy5.5-GdMnO of this embodiment 3 -FA, Cy5.5-GdMnO 3 -PEG FTIR cha...

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Abstract

The invention discloses a dual-mode probe applicable to magnetic resonance imaging and near infrared imaging, wherein a gadolinium-doped manganese carbonate nanoparticle serves as a crystal core, and a silylating reagent, polyethylene glycol, near infrared dye and a target for targeting brain gliomas are combined on the surface of the crystal core. Furthermore, the invention discloses a preparation method of the gadolinium-doped manganese carbonate magnetic resonance imaging contrast agent, a modifying method of a fluorescent probe and application of the probe to targeting brain glioma imaging. The dual-mode probe disclosed by the invention is highly dispersed and stable in water, is applicable to dual-mode imaging of tumors and also can be applied to a drug carrier, cell tracing and gene transfection.

Description

technical field [0001] The present invention relates to a dual-mode imaging probe of gadolinium-doped manganese carbonate that can be used for magnetic resonance imaging (MRI) and near-infrared imaging, and more particularly relates to a dual-mode imaging probe for gadolinium-doped manganese carbonate for brain glioma probe. [0002] The present invention also relates to a method for preparing the above-mentioned gadolinium-doped manganese carbonate dual-mode imaging probe for brain glioma. [0003] The present invention also relates to the application of the above-mentioned gadolinium-doped manganese carbonate dual-mode imaging probe as a fluorescent probe in near-infrared imaging, as a contrast agent in magnetic resonance imaging, and in drug carriers, cell tracer drugs and gene transfection. Background technique [0004] The use of contrast agents in magnetic resonance imaging (MRI) can enhance the contrast between pathological and normal tissues. 1997MnCl 2 Tetrahydra...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/10A61K49/12A61K49/14A61K49/00A61K48/00A61K47/48A61K47/34
Inventor 叶玲邵晨李帅顾微
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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