Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current

A technology of microfluidic chip and anode current, applied in the direction of material electrochemical variables, etc., to achieve integration and portability, reduce equipment or production costs, and reduce equipment dependence

Inactive Publication Date: 2015-01-07
XI AN JIAOTONG UNIV
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Problems solved by technology

[0004] In order to overcome the defects of the above-mentioned prior art, the object of the present invention is to provide a paper-based microfluidic chip anode current detection method for the biological toxicity of water pollutants, based on the principle of microbial respiratory chain-electrochemical detection of the BQ system, on the screen printing PDMS On the paper-based microfluidic chip of the hydrophilic microchannel and carbon slurry three-electrode system, a small piece of cellulose membrane is used to conduct the trap on the hydrophilic microchannel to control the release and flow time of the liquid flow, so as to realize the separation of the culture medium from the cells, Cl

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  • Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current
  • Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current
  • Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0030] A paper-based microfluidic chip anode current detection method for the biological toxicity of water pollutants, comprising the following steps,

[0031] Step 1. Make a paper-based microfluidic chip first. For the manufacturing steps, refer to figure 2 , the final chip structure refers tofigure 1 ,

[0032] (1.1), earlier use CorelDRAW 9 drawing software design to comprise the three electrodes of counter electrode 4, working electrode 5 and reference electrode 6, three electrodes are processed into the first screen plate 21 of 200 orders, use on Whatman No. 1 chromatographic paper 22 fronts Carbon paste (Henkel) screen printing, baked in an oven at 150°C for 1 hour; obtain chromatographic paper comprising three electrodes;

[0033] (1.2), then use drawing software design to include five microchannels and sample injection area 3, five microcha...

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Abstract

The invention discloses a method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current. The method is characterized in that a paper-based micro-fluidic chip is prepared and then water pollutant biotoxicity is determined by the paper-based micro-fluidic chip. According to a microbial respiratory chain-based BQ system electrochemical detection principle, on the paper-based micro-fluidic chip of a screen printing PDMS hydrophilic microchannel and carbon paste three-electrode system, a steam trap of the hydrophilic microchannel is dredged by a small piece of a cellulose membrane so that liquid flow release time and flowing time are controlled and separation and cleaning of a nutrient solution and cells and cell incubation by pollutants are realized and thus the interference produced by current signal detection device anode current-based quantitative determination of pollutant toxicity is reduced. The method has the advantages of light quality, carrying convenience, low cost, one-step analysis, small sample volume and fast analysis speed and is suitable for biotoxicity detection in occasions of a remote area, field on-site water pollution, soil pollution, food safety and space carrying.

Description

technical field [0001] The invention relates to the technical field of microfluidic analysis applied to microbial detection of pollution toxicity, in particular to a method for detecting the biological toxicity of water pollutants by anode current of a paper-based microfluidic chip. Background technique [0002] Microfluidic chip, as the core technology of the micro-total analysis system proposed in the 1990s, is one of the fastest-growing fields at present, and paper microfluidic chip is a new form of microfluidic chip developed recently. . Paper-based microfluidics uses chromatographic paper as the chip material, and is fabricated using techniques such as photolithography, plasma oxidation, inkjet printing, wax printing and printing PDMS (Xu Li, et al., 2012, 6, 1932-1058) . Paper-based microfluidic chip characterized by the application of test paper and lateral flow, due to its portability, batch printing, simple preparation process, no need for complex peripheral equip...

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Application Information

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IPC IPC(8): G01N27/26
Inventor 梁恒许朝萍杨峥峥刘倩欧阳良飞孙欢杨水云徐文
Owner XI AN JIAOTONG UNIV
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