Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current

Abstract

The invention discloses a method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current. The method is characterized in that a paper-based micro-fluidic chip is prepared and then water pollutant biotoxicity is determined by the paper-based micro-fluidic chip. According to a microbial respiratory chain-based BQ system electrochemical detection principle, on the paper-based micro-fluidic chip of a screen printing PDMS hydrophilic microchannel and carbon paste three-electrode system, a steam trap of the hydrophilic microchannel is dredged by a small piece of a cellulose membrane so that liquid flow release time and flowing time are controlled and separation and cleaning of a nutrient solution and cells and cell incubation by pollutants are realized and thus the interference produced by current signal detection device anode current-based quantitative determination of pollutant toxicity is reduced. The method has the advantages of light quality, carrying convenience, low cost, one-step analysis, small sample volume and fast analysis speed and is suitable for biotoxicity detection in occasions of a remote area, field on-site water pollution, soil pollution, food safety and space carrying.

Description

technical field [0001] The invention relates to the technical field of microfluidic analysis applied to microbial detection of pollution toxicity, in particular to a method for detecting the biological toxicity of water pollutants by anode current of a paper-based microfluidic chip. Background technique [0002] Microfluidic chip, as the core technology of the micro-total analysis system proposed in the 1990s, is one of the fastest-growing fields at present, and paper microfluidic chip is a new form of microfluidic chip developed recently. . Paper-based microfluidics uses chromatographic paper as the chip material, and is fabricated using techniques such as photolithography, plasma oxidation, inkjet printing, wax printing and printing PDMS (Xu Li, et al., 2012, 6, 1932-1058) . Paper-based microfluidic chip characterized by the application of test paper and lateral flow, due to its portability, batch printing, simple preparation process, no need for complex peripheral equip...

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Problems solved by technology

[0004] In order to overcome the defects of the above-mentioned prior art, the object of the present invention is to provide a paper-based microfluidic chip anode current detection method for the biological toxicity of water pollutants, based on the principle of microbial respiratory chain-electrochemical detection of the BQ system, on the screen printing PDMS On the paper-based microfluidic chip of the hydrophilic microchannel and carbon slurry three-electrode system, a small piece of cellulose membrane is used to conduct the trap on the hydrophilic microchannel to control the release and flow time of the liquid flow, so as to realize the separation of the culture medium from the cells, Cleaning and pollutant incubation cells, etc., so as to reduce the interference when using the anode current of the current signal detection device to quantitatively determine the toxicity of pollutants, overcome the defects of traditional methods, and have light weight, portability, low cost, one-time analysis, all The size of the sample is small and the analysis speed is fast. It is suitable for the detection of biological toxicity in remote areas, field water pollution, soil pollution, food safety, and space loading, so as to be used in the fields of environmental protection, life sciences, medicine, and biochemistry. The biotoxicity assay provides a new type of equipment and a new method

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