Dual-detection biosensing chip and preparation method thereof and DNA detection method

A biosensing and dual-detection technology, applied in the field of bioelectronics, electrochemistry and optical sensing, can solve the problems of time-consuming, cumbersome process and high cost, and achieve the effects of reducing false positives, accurate detection results and short time-consuming

Active Publication Date: 2015-01-14
盐城市凤凰园科技发展有限公司
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Problems solved by technology

[0004] But at present, in order to meet the quantitative or qualitative detection requirements of different target substances, it is necessary to carry out multiple detections such as optical biological detection and electrochemical biological detection at the same time to determine the accurate information of the analyte; For chemical or optical sensor chips, each sensor chip can only realize a single type of detection, resulting in the need to repeat the detection process several times, which is time-consuming and costly; the corresponding single sensor chip needs to be prepared separately, and the process is cumbersome

Method used

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  • Dual-detection biosensing chip and preparation method thereof and DNA detection method
  • Dual-detection biosensing chip and preparation method thereof and DNA detection method
  • Dual-detection biosensing chip and preparation method thereof and DNA detection method

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preparation example Construction

[0035] The present invention further proposes the preparation method of the above-mentioned dual detection biosensor chip, comprising the following steps:

[0036] S10, preparing a nano-gold electrode;

[0037] S20, assembling a dual-detection biosensor chip;

[0038] S30, immobilizing the DNA probe on the nano-gold electrode;

[0039] Among the above-mentioned steps of the present invention, step S10 is to prepare a nano-gold electrode, and the following steps can be used in its specific implementation. For details, see Figure 2-7 The schematic diagram shown:

[0040] S11, obtaining an ITO glass 211, and cleaning and drying the surface thereof for use;

[0041] S12, coating a resist layer 212 on the surface of the ITO glass 211;

[0042] S13, generating nanoscale-sized grooves 214 on the surface of the resist layer 212;

[0043] S14, depositing a protective layer 215 on the non-groove surface of the resist layer 212;

[0044] S15, performing plasma etching on the ITO gla...

Embodiment 1

[0070] S11, choose an ITO circular glass substrate with a thickness of 1 mm and a diameter of 1 cm, ultrasonically clean it with ammonia water, ethanol and distilled water for 3 minutes, and dry it with nitrogen;

[0071] In this step, the ITO glass substrate is selected as a circular shape for the convenience of electrode assembly and electrode preparation; of course, it can also be replaced with other regular or irregular shapes according to the needs, not limited to the circle shaped shape.

[0072] S12, coating a single-layer imprinting resist such as resin etc. on the ITO circular glass substrate cleaned in step S11, forming a resin layer on the ITO circular glass substrate;

[0073] S13, obtain an embossing plate, the embossing surface of the embossing plate has a nanoscale convex model; after the embossing surface of the embossing plate is pressed against the resin layer and the ITO circular glass substrate, the embossing plate is removed , then a groove matching the c...

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Abstract

The invention provides a dual-detection biosensing chip. The chip comprises a substrate and a three-electrode system arranged on the substrate, wherein the three-electrode system comprises a biosensing electrode, an auxiliary electrode and a reference electrode; the biosensing electrode is a nano-gold electrode, the nano-gold electrode comprises a transparent conducting base on the substrate, a plurality of regularly arranged nano-gold particles are arranged on the surface of the transparent conducting base. According to the dual-detection biosensing chip, a work electrode in the three-electrode system adopts the nano-gold electrode, the electrode structure of the nano-gold particle can be used for performing optical biological detection on the local surface plasma; and the transparent conducting base is an N-shaped semiconductor, the transparent feature cannot influence the optical detection simultaneously when the electrochemical biological detection is performed.

Description

technical field [0001] The invention belongs to the technical field of bioelectronic electrochemistry and optical sensing, and in particular relates to a dual-detection biosensing chip combined with electrode surface electrochemical signals and localized surface plasmon resonance, as well as a preparation method and a detection method thereof. Background technique [0002] Localized Surface Plasmon Resonance (LSPR, Localized Surface Plasmon Resonance) is a metal nanoparticle or a discontinuous metal nanostructure excited by incident light, and its internal free electrons synergistically oscillate to produce localized surface plasmon resonance. The surface of the metal nanostructure The localized electromagnetic field of is greatly enhanced, exhibiting strong surface plasmon resonance absorption. The biosensing chip uses the surface of nanoparticles to modify specific capture probes. The hybridization / affinity binding between the detection substance and the capture probes wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N21/31G01N27/48C12Q1/68
Inventor 李科铮吴宇亮于洪宇
Owner 盐城市凤凰园科技发展有限公司
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