Nisin separation and extraction method

Abstract

The invention relates to a nisin separation and extraction method. The method comprises the following steps: blowing air to nisin fermentation liquor at a certain flow rate by adopting mixed gas of air and carbon dioxide at a certain volume ratio and a heated temperature so as to separate nisin from the foam fermentation liquor; collecting and defoaming to obtain a high purity nisin concentrated solution; adding a carrier and a stabilizer into the concentrated solution; and spraying and drying to prepare a nisin food preservative with qualified valence. High concentration macromolecular proteins such as thalli in the fermentation liquor subjected to hot air flows are easily separated from the fermentation liquor in form of precipitate and the precipitate is vacuum-dried to prepare thallus powder as nutritional feed. The method provided by the invention not only can be used for omitting labor-wasting, time-wasting and material-wasting high temperature pre-treatment requiring addition of massive hydrochloric acid solution into the fermentation liquor at present, but also avoids a process of filtering and collecting thallus residues by using a high cost ceramic membrane and filtering to remove thalli by using a diatomite sheet frame which generates a lot of solid residues, with high strength in a large site for a long time.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation industry, in particular to a method for separating and extracting nisin. Background technique [0002] The separation and extraction of nisin, which is simple and easy to perform and has zero pollution, has always been the method sought by nisin manufacturers. The separation and extraction of nisin by using a mixture of heated air and carbon dioxide has not been reported in the literature so far. [0003] Diatomaceous earth plate and frame filtration is mostly used in the fermentation industry to remove bacteria, which requires a large area of ​​equipment, labor and material, and time-consuming, and produces a large amount of solid waste residue; while recycling bacteria mostly uses ceramic membrane filtration, but its cost is high and time-consuming long. Contents of the invention [0004] The purpose of the present invention is to overcome the deficiencies of the prior art and...

AI Technical Summary

Problems solved by technology

[0002] The separation and extraction of nisin, which is simple and easy to perform and has zero pollution, has always been the method sought by nisin manufacturers. The separation and extraction of nisin by using a mixed gas of heated air and carbon dioxide has not been reported in the literature so far.

[0003] Diatomaceous earth plate and frame filtration is mostly used in the fermentation industry to remove bacteria, which requires a large area of ​​equipment, labor and material, and time-consuming, and produces a large amount of solid waste residue; while recycling bacteria mostly uses ceramic membrane filtration, but its cost is high and time-consuming long