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Brilliant green lactic acid medium and application thereof

A culture medium and bright green technology, applied in the field of microorganisms, can solve problems such as inability to infer and calculate, and achieve the effects of low cost, easy observation, and rapid bacterial growth.

Inactive Publication Date: 2015-01-21
QINGDAO KANGHEWEIYE COMML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of bright green lactic acid culture medium, the component of described culture medium comprises EC broth, 2.0g / L ox bile salt, 0.012g / L bright green, 3.0g / L manganese sulfate, 15.0g / L agar, pH value 7.1-7.3;

[0023] On the basis of the above scheme, the EC broth contains 20.0 g of tryptone, 10.0 g of lactose, 2.0 g of potassium dihydrogen phosphate, 5.0 g of sodium chloride, 1.5 g of bile salt No. 3, and hydrogen phosphate per liter of EC broth. Dipotassium 4.0g, the rest is distilled water.

[0024] The invention also discloses the bright green lactic acid culture medium, which is used for the detection of Escherichia coli in drinking natural mineral water.

[0025] Weigh 22.0g of this product, dissolve it in 1000ml of distilled water with heating and stirring, dispense it into test tubes with small catheters, 10ml per tube, autoclave at 115°C for 20 minutes, and set aside.

[0026] Incubate at 36±1°C for 18-24 hours

[0027] Quality Control Strains...

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PUM

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Abstract

The invention belongs to the field of microorganisms and in particular relates to a brilliant green lactic acid medium. The medium comprises the following components: EC broth, 2.0g / L of bile salt, 0.012g / L of bright green, 3.0g / L of manganese sulfate and 15.0g / L of agar, wherein the pH value of the medium is 7.1-7.3; the EC broth per liter contains 20.0g of tryptone, 10.0g of lactose, 2.0g of potassium dihydrogen phosphate, 5.0g of sodium chloride, 1.5g of bile salt No.3, 4.0g of dipotassium hydrogen phosphate and the balance of distilled water. The brilliant green lactic acid medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a bright green lactic acid culture medium and its application. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10
Inventor 王玉静刘永祥
Owner QINGDAO KANGHEWEIYE COMML