Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb

A technology for joint detection of hepatitis B and immunochromatography, applied in measuring devices, analytical materials, biological testing, etc.

Inactive Publication Date: 2015-01-21
CHENGDU LINGYU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention discloses a quantum dot immunochromatographic test strip and a method thereof for synchronous quantitative joint detection of three items of hepatitis B to solve the problem of simultaneous rapid quantitative detection of multiple indicators of hepatitis B markers

Method used

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  • Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb
  • Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb
  • Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Quantum dot immunochromatography test strip structure for simultaneous quantitative joint detection of HBsAg, HBeAg, and HBcAb

[0042] combine figure 1 be explained.figure 1 Among them, the quantum dot immunochromatographic test strip for the simultaneous quantitative joint detection of the three items of hepatitis B includes a sample pad 1, a red blood cell filter membrane 2, a marker pad 3, an analysis membrane 7, and Absorbent pad6.

[0043] The marking pad 3 of the test strip is a glass fiber membrane. The analysis membrane 7 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose / acetate mixed membrane, on which there are a detection zone (ie T zone) 4 and a quality control zone (ie C zone) 5 . Described backing 8 is polyester or plastic plate.

[0044] The marker pad 3 is coated with CdSe / ZnS QD 526 Labeled mouse anti-HBsAg monoclonal antibody (Mouse anti-HBsAg McAb -CdSe / ZnS QDs 526 ), CdSe / ZnS QDs 615 Labeled mouse anti-HBeAg monoc...

Embodiment 2

[0045] Example 2: The preparation method of the quantum dot immunochromatography test strip for simultaneous quantitative joint detection of HBsAg, HBeAg, and HBcAb

[0046] The preparation method of described test strip comprises the steps:

[0047] A. Quantum dot conjugated antibody / antigen:

[0048] a) Take water-soluble quantum dots CdSe / ZnS QD with emission wavelengths of 526nm, 615nm, and 566nm 526 、CdSe / ZnS QD 615 、CdSe / ZnS QD 566 Use PBS buffer to adjust pH=6-9, respectively add EDC (l-(3-dimethylaminopropyl)-3-ethylcarbodiamine hydrochloride) and NHS (N-hydroxythiosuccinic acid imine) activated at room temperature for 10-60min;

[0049] b) Mouse anti-HBsAg monoclonal antibody (Mouse anti-HBsAg McAb ), mouse anti-HBeAg monoclonal antibody (Mouse anti-HBeAg McAb ), HBcAg vortex shaking reaction for 0.5-3h;

[0050] c) Add bovine serum albumin (BSA) respectively, and block the reaction in the dark for 0.5-2h;

[0051] d) Each product was purified by centr...

Embodiment 3

[0061] Example 3: Preparation method of the standard curve for the simultaneous quantitative joint detection of HBsAg, HBeAg, and HBcAb by using the quantum dot immunochromatographic test strip for simultaneous quantitative joint detection of hepatitis B three items

[0062] （a） Take HBsAg, HBeAg, HBcAb standard products respectively with phosphate buffered solution (PBS) to make several parts of standard product serial concentration in the way of doubling dilution.

[0063] （b） Add the concentration of each standard substance dropwise on 10 quantum dot immunochromatography test strips and detect with a detector under the same conditions (that is, use 10 quantum dot immunochromatography test strips for each standard substance concentration on the same 10 times with the detector under the conditions), and read the fluorescence intensity of the T band (OD t ) vs. C-band fluorescence intensity (OD c ), to get the mean and OD t / OD c ratio.

[0064] （c） Take the concentrati...

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Abstract

The invention particularly relates to a quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and a method for the synchronous and quantitative joint inspection of the HBsAg, the HBeAg and the HBcAb. A label pad (3) of the test strip is coated with a mixture of a rat anti-HBsAg monoclonal antibody, a rat anti-HBeAg monoclonal antibody and HBcAg of corresponding labels of quantum dots with different wavelengths; a T belt (4) of an analyzing membrane (7) is coated with a mixture of a rabbit anti-HBsAg antibody, a rabbit anti-HBeAg antibody and the HBcAg; and a C belt (5) of the analyzing membrane (7) is coated with a goat anti-rat antibody. An HBsAg, HBeAg and HBcAb standard curve is stored and mounted on the test strip by an electronic label. The test strip adopts a detector with a signal detection function to read the standard curve stored by the electronic label and combines corresponding fluorescence intensity of a sample to be detected, which is measured by the detector, so as to synchronously and quantitatively detect the concentrations of the HBsAg, the HBeAg and the HBcAb in a blood sample.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and in particular relates to a quantum dot immunochromatographic test strip and a method thereof for simultaneous quantitative joint detection of three items of hepatitis B (HBsAg, HBeAg, and HBcAb). Background technique [0002] Hepatitis B is a major infectious disease that seriously endangers human health. my country is a country with a large number of hepatitis B infections. More than 600 million people have been infected with hepatitis B, and about 130 million people are carrying hepatitis B virus. The prevention and treatment of hepatitis B is of great significance. At present, hepatitis B markers are not yet capable of simultaneous and rapid quantitative detection of multiple indicators. The traditional enzyme-linked immunosorbent assay (ELISA) can only detect one indicator at a time, and the test results of each indicator can be obtained after performing multiple parallel detection proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531G01N21/64
CPCG01N33/5761G01N33/52G01N33/531
Inventor 王春英马义才侯飞
Owner CHENGDU LINGYU BIOTECH
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