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Breeding method of carmine radish

A technology of rouge radish and single plant, which is applied in the field of rouge radish breeding, which can solve the problems of rough taste, unsuitable for fresh food, and changing the nutritional quality of rouge radish, and achieve the effects of good light exposure, strong stress resistance, high yield and stable yield

Active Publication Date: 2015-01-28
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Problems solved by technology

[0005] Technical problem to be solved: Aiming at the problems of the existing carmine radish with dense texture, rough taste and unsuitable for fresh food, the present invention provides a breeding method of carmine radish, adopts colchicine seedling stage treatment, changes the nutritional quality of carmine radish, and passes 6 Generation system selection to obtain Rouge radish strains suitable for fresh eating and crisp taste

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  • Breeding method of carmine radish
  • Breeding method of carmine radish
  • Breeding method of carmine radish

Examples

Experimental program
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Effect test

Embodiment 1

[0016] The self-reserved species of rouge radish collected from Fuling, Sichuan were selected, and the botanical characters of the material populations were different and the mixture was serious.

[0017] (1) According to the breeding goals of fleshy root red pigment content ≥ 140mg / 100g fresh weight, combined with crisp taste, glucoraphanin content < 70mg / 100g fresh weight, resistance to hollow and split roots, cylindrical and straight root shape, directional optimization Single plant, 5-6 generations of self-purification and breeding.

[0018] (2) After 5-6 generations of self-purification, select a strain with a fleshy root pigment content ≥ 140mg / 100g fresh weight, a crisp taste, and strong resistance to hollowing and root splitting, and use a 0.1% colchicine solution at the seedling stage. After 72 hours of mutagenesis treatment, ploidy identification was carried out according to the plant type, leaf type, chloroplast number of stomatal guard cells and stomatal size after...

Embodiment 2

[0024] (1) According to the breeding goals of red pigment content in fleshy roots ≥ 140mg / 100g fresh weight, combined with crisp taste and less spicy taste, resistance to hollow and cracked roots, cylindrical and straight root shape, directional selection of individual plants, 5-6 generations from Submit purification and selection.

[0025] (2) After 5-6 generations of self-purification, select a strain with a fleshy root pigment content ≥ 140mg / 100g fresh weight, a crisp taste, and strong resistance to hollowing and root splitting, and use a 0.2% colchicine solution at the seedling stage to induce After 48 hours of treatment, the ploidy was identified according to the plant type, leaf type and chromosome number after one month of observation, and the mutated and unmutated plants were reserved for individual seeds.

[0026] (3) The stable inbred line of rouge radish with no ploidy variation, long gourd-shaped fleshy root, 156 mg / 100g fresh weight of fleshy part pigment, and sp...

Embodiment 3

[0031](1) According to the breeding goals of red pigment content in fleshy roots ≥ 140mg / 100g fresh weight, combined with crisp taste and less spicy taste, resistance to hollow and cracked roots, cylindrical and straight root shape, directional selection of individual plants, 5-6 generations from Submit purification and selection.

[0032] (2) After 5-6 generations of self-purification, select a strain with a fleshy root pigment content ≥ 140mg / 100g fresh weight, a crisp taste with less spicy taste, resistance to hollow and cracked roots, and cylindrical and straight roots, and the concentration is 0.15 % colchicine solution was used for mutagenesis treatment at the seedling stage for 72 hours; after one month of observation, the ploidy identification was carried out according to the plant type, leaf type and flow cytometry.

[0033] (3) A stable inbred line of rouge radish with no ploidy variation, short cylindrical fleshy root, 157mg / 100g fresh weight of fleshy part pigment,...

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Abstract

The invention discloses a breeding method of carmine radish. The breeding method comprises the following steps: selecting different types of carmine radishes, selectively choosing excellent strains according to the breeding target, carrying out selfing for 5 to 6 generations, carrying out breeding selection; after selfing for 5 to 6 generations, selecting different types of stable selfing lines, subjecting the selected selfing lines to a colchicine induction treatment in the seedling period, then carrying out ploidy identification; after the ploidy identification, selecting the excellent strains that do not generate variation, hybridizing the selected excellent strains; selecting the excellent strains after the hybridization, carrying out directive breeding through a pedigree breeding method, and obtaining the stable carmine radish selfing lines after 6 generations. The obtained carmine radish has the advantages of high pigment content, crispy taste, less spicy taste, hollow and cracked root resistance, large and straight shape, and suitability for direct eating.

Description

technical field [0001] The invention relates to a breeding method of rouge radish, which belongs to the technical field of plant breeding. Background technique [0002] Carmine radish ( Raphanus Sativus L. Sinoruber Makino ) is a local variety of radish in the genus Brassicaceae. Its fleshy root is rich in nutrients, and its content of protein, calcium, iron, VB1 and VB2 is higher than other types of radish, and its texture is denser. It is suitable for processing various dried radishes. It is bright red, tender and crisp. At the same time, the fleshy root bark and heart are all red. The content of radish red pigment is high, and the quality and solubility of the pigment are better than other carrots. Therefore, red radish is an ideal raw material for extracting natural edible red pigment. Crops have been cultivated in a relatively large area in Southwest my country. Radish red pigment extracted from rouge radish is a natural food coloring, which is safe, non-toxic, rich i...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
Inventor 张振超毛忠良吴国平姚悦梅戴忠良潘跃平秦文斌潘永飞王建华肖燕孙春青马志虎孙国胜
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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