Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of preparing collagen

A collagen and protease technology, applied in the biological field, can solve problems such as complex process, low extraction rate, heavy metal residue, etc., and achieve the effect of large contact area, improved extraction rate, and rapid enzymatic hydrolysis

Active Publication Date: 2015-01-28
HAINAN YUNHAO BIOLOGICAL TECH CO LTD
View PDF7 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the shortcomings of the current collagen production process, the present invention provides a method for preparing collagen from fish skin and scales, which solves the problems of serious environmental pollution, heavy metal residues, complicated process, and low extraction rate in the current collagen production process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of preparing collagen
  • Method of preparing collagen
  • Method of preparing collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Take the fish skin and scales that are leftovers from aquatic product processing, wash them with water, add water 6 times the weight of the fish skin and scales, stir and heat to above 90°C, keep above 90°C for 30 minutes, filter and dry, and use the fish skin and scales for later use;

[0056] 2. Take the treated fish skin scales, add 0.05mol / L citric acid solution 4 times the weight of fish skin scales, ultrasonically treat for 4 hours, adjust the pH value to 6.0-7.5 with 10.0mol / L NaOH, filter and dry, Wash with water, filter dry, and set aside;

[0057] 3. Add 3 times the amount of water to the fish skin and scales, use a colloid mill to grind the fish skin and scale slurry, adjust the pH value to 8.0-8.5 with 10.0mol / L NaOH, and maintain the pH value within the range of 8.0-8.5, add fish skin Alcalase 2.4L FG with 0.1% fish scale weight, enzymatically hydrolyzed at 50°C-55°C for 100min, after enzymolysis, heat to above 90°C for 10min to inactivate the enzyme, co...

Embodiment 2

[0061] 1. Take the fish skin and scales that are leftovers from aquatic product processing, wash them with tap water, add water 12 times the weight of the fish skin and scales, stir and heat to above 90°C, keep above 90°C for 90 minutes, filter and dry, and use the fish skin and scales for later use;

[0062] 2. Take the treated fish skin and scales, add 0.05mol / L citric acid solution 12 times the weight of fish skin and scales for ultrasonic treatment for 8 hours, and use 10.0mol / L Na 2 CO 3 Adjust the pH value to 6.0-7.5, filter dry, wash with tap water, filter dry, and set aside;

[0063] 3. Add 8 times the amount of purified water to the fish skin and scale, use a colloid mill to grind the fish skin and scale slurry, and use 10.0mol / L Na 2 CO 3 Adjust the pH value to 8.0-8.5, maintain the pH value in the range of 8.0-8.5, add 0.8L of Neutrase with 0.4% weight of fish skin and scale, enzymatically hydrolyze at 50°C-55°C for 60min, and heat to above 90°C for 10min after en...

Embodiment 3

[0067] 1. Take the fish skin and scales that are leftovers from aquatic product processing, wash them with water, add 8 times the weight of fish skin and scales, water, stir and heat to above 90°C, keep above 90°C for 60 minutes, filter and dry, and use the fish skin and scales for later use;

[0068] 2. Take the treated fish skin and scale, add 0.05mol / L citric acid solution with a weight of 8 fish skin and scale, and ultrasonically treat it for 6 hours, adjust the pH value to 6.0-7.5 with 10.0mol / L NaOH, filter to dry, and wash with water Once, drained and set aside;

[0069] 3. Add 6 times the amount of purified water to the fish skin and scales, use a colloid mill to grind the fish skin and scale slurry, adjust the pH value to 8.0-8.5 with 10.0mol / L NaOH, and maintain the pH value within the range of 8.0-8.5, add fish Alcalase 2.4L FG with 0.2% weight of skin fish scales, 50°C-55°C for 180min, after enzymolysis, heat to above 90°C for 10min, carry out enzyme inactivation, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Average molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biotechnology and discloses a method of preparing collagen by using fish skins and scales as raw materials. The method of preparing the collagen comprises the following steps: cleaning the fish skins and scales, adding a 0.01-0.1mol / L citric acid solution, carrying out ultrasonic treatment for 4-8 h, regulating the pH value to be 6.0-7.5 by adding an alkali solution, and drying and filtering; adding water in the fish skins and scales which are dried and filtered, milling into fish skin and scale slurry by a colloid mill, regulating the pH value to be 8.0-8.5 by adding the alkali solution, adding alkaline protease for enzymatic hydrolysis for 60-180 min, and carrying out inactivation on the protease to obtain a collagen solution. According to the method disclosed by the invention, the fish skins and scales are degreased and deashed by the technology of combining citric acid treatment and ultrasonic waves; meanwhile, with adoption of the technology of combining colloid mill treatment and alkaline protease enzymatic hydrolysis, enzymatic hydrolysis is rapid, and protein macromolecules are completely hydrolyzed into small molecules, thus increasing extraction rate of the collagen and enabling the prepared collagen product to be safe and natural.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing collagen, in particular to a method for preparing collagen by using fish skin and scale as raw materials. Background technique [0002] Collagen, also known as collagen, is a structural protein of the extracellular matrix. The English name "collagen" evolved from Greek. It is a polysaccharide protein that is white and contains a small amount of galactose and glucose. It is an extracellular matrix (ECM ) main component, accounting for about 85% of the collagen fiber solids. Collagen is a macromolecular protein ubiquitous in animals. It mainly exists in the connective tissues (bone, cartilage, skin, tendon, toughness, etc.) of animals. It is the most abundant and widely distributed functional protein in mammals. , accounting for 25% to 30% of protein in mammals, equivalent to 6% of body weight. In many marine organisms, such as the skin of fish, its...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06
Inventor 伍曾利
Owner HAINAN YUNHAO BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com