Unlock instant, AI-driven research and patent intelligence for your innovation.

A kit for detecting animal-derived components in feed and its detection method

A technology of animal-derived ingredients and kits, which is applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of time-consuming and low efficiency, and achieve the prevention of feed adulteration, small molecular weight, and reliable detection Effect

Active Publication Date: 2016-10-05
NORTHWEST UNIVERSITY FOR NATIONALITIES
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing animal-derived feed testing methods are time-consuming and inefficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kit for detecting animal-derived components in feed and its detection method
  • A kit for detecting animal-derived components in feed and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 A kit for the detection of animal-derived components in feed, comprising

[0044] —Detection primer solution: consist of outer primer I with a concentration of 4~6 µmol / L, outer primer II with a concentration of 4~6 µmol / L, inner primer I with a concentration of 32~48 µmol / L and a concentration of 32~48 µmol / L internal primer II prepared;

[0045] - Bst DNA polymerase with strand displacement activity: the concentration is 7~9U / μL;

[0046] —10× reaction buffer: Tris-HCl of 200 mmol / L and pH=8.8, 100 mmol / L KCl, 100 mmol / L (NH 4 ) 2 SO 4 , 40~100 mmol / L MgSO 4 , 6~14 mol / L betaine;

[0047] —dNTPs solution: It is formed by mixing equal volumes of four deoxyribonucleotide solutions of dATP, dCTP, dGTP and dTTP with a concentration of 10 mmol / L;

[0048] —Positive DNA control sample: Escherichia coli plasmid DNA containing highly conserved DNA sequences of vertebrate mitochondria, or total DNA samples of pigs, cattle, sheep, chickens, ducks and fish commo...

Embodiment 2

[0057] Example 2 A detection method of a kit for detection of animal-derived components in feed, comprising the following steps:

[0058] (1) Extract the DNA of the sample to be tested according to the conventional method;

[0059] ⑵ Prepare the LAMP detection reaction system for the sample to be tested:

[0060] Add 2~5 μL of DNA to be tested, 1.5 μL of detection primer solution, 1 μL of BstDNA polymerase, 2.5 μL of 10× reaction buffer, and 3 μL of dNTPs solution into 200 μL PCR reaction tube Ⅰ, and make up with sterilized deionized water to 25 μL;

[0061] (3) Prepare the LAMP detection reaction system for the control sample:

[0062] Add 2~5 μL of positive DNA control sample, 1.5 μL of detection primer solution, 1 μL of Bst DNA polymerase, 2.5 μL of 10× reaction buffer, and 3 μL of dNTPs solution into 200 μL PCR reaction tube II, and make up with sterilized deionized water. Make up to 25μL;

[0063] Add 2~5 μL of negative DNA control sample, 1.5 μL of detection primer s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a kit for detecting animal-derived components in a feed. The kit comprises a detection primer solution prepared from an outer primer I, an outer primer II, an inner primer I and an inner primer II, a BstDNA polymerase with chain displacement activity, a 10*reaction buffer, a dNTPs solution, a positive DNA control sample, a negative DNA control sample and a color developing agent. Meanwhile, the invention also discloses a detection method of the kit. The kit disclosed by the invention has the characteristics of high specificity, high speed, high efficiency and low detection cost, is simple and convenient to operate and is suitable for rapid field detection.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for detection of animal-derived components in feed and a detection method thereof. Background technique [0002] Feed safety hazards will lead to food safety problems, endanger human health, and are not conducive to social stability. They are closely related to animal production, environmental pollution and human health, and have become a hot issue of global concern. In recent years, food exposure incidents contaminated by feed raw materials and feed additives have continued. With the occurrence of animal-derived food safety incidents caused by feed safety issues, a series of incidents that endanger human health and life safety have broken out. Mad cow disease is one of the most harmful ones. There is currently no cure. In order to block the spread of "mad cow disease" through the breeding and production process and eliminate the potential safety hazards caused by the use of animal-derive...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6844C12Q1/6888C12Q2531/119
Inventor 臧荣鑫徐红伟
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com