Preparation method of oral subunit vaccine for porcine epidemic diarrhea virus

A porcine epidemic diarrhea and subunit vaccine technology, which is applied in the field of animal biomedical engineering, can solve the problems of easy degradation and inability to take the vaccine orally, and achieve the effects of simple preparation process, low preparation cost, and non-degradable

Active Publication Date: 2015-02-18
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defect that porcine epidemic diarrhea virus subunit vaccine cannot be taken orally and is easily degraded in the gastrointestinal tract in t

Method used

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  • Preparation method of oral subunit vaccine for porcine epidemic diarrhea virus
  • Preparation method of oral subunit vaccine for porcine epidemic diarrhea virus
  • Preparation method of oral subunit vaccine for porcine epidemic diarrhea virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of porcine PEDV subunit vaccine coated with chitosan:

[0029] S1. Configuration of chitosan solution: Dissolve chitosan with a deacetylation degree of 85% in 0.5% (v / v) acetic acid to prepare a 0.2% (w / v) chitosan solution;

[0030] S2. Take 4 ml of the chitosan solution prepared in step S1, add 1 ml of porcine epidemic diarrhea virus antigen COE protein dropwise while stirring, and stir for 15 min after adding the protein;

[0031] S3. Add 2 ml of sodium tripolyphosphate with a concentration of 0.2% (W / V) into the S2 solution, and continue stirring for 5 minutes to obtain the vaccine.

[0032] Among them, 60 μg / mL porcine epidemic diarrhea virus antigen COE protein was obtained by diluting 500 μg / mL porcine epidemic diarrhea virus antigen COE protein stock solution with 0.9% normal saline, and porcine epidemic diarrhea virus core antigen COE protein stock solution was obtained from South China Obtained by the poultry laboratory of the College of Anima...

Embodiment 2

[0034] Preparation of porcine PEDV subunit vaccine coated with chitosan:

[0035] S1. Configuration of chitosan solution: Dissolve chitosan with a deacetylation degree of 80% in 0.6% (v / v) acetic acid to prepare a 0.2% (w / v) chitosan solution;

[0036] Other steps are with embodiment 1.

Embodiment 3

[0038] Preparation of porcine PEDV subunit vaccine coated with chitosan:

[0039] S1. Configuration of chitosan solution: Dissolve chitosan with a deacetylation degree of 85% in 0.3% (v / v) acetic acid to prepare a 0.2% (w / v) chitosan solution;

[0040] Other steps are with embodiment 1.

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Abstract

The invention belongs to the field of biological medicine engineering, and particularly discloses a preparation method of an oral subunit vaccine for a porcine epidemic diarrhea virus. The prepared subunit vaccine can be directly used as an oral vaccine to immune a mouse, and the mouse can be stimulated to generate stronger humoral immune response and cellular immune response. The vaccine is not degraded easily in the stomach and intestines, the immune effect is remarkable, the preparation process is simple, and the oral subunit vaccine plays an active role in relieving harm caused by the porcine epidemic diarrhea virus to the pig industry and has important practical significance in promoting healthy development of the pig industry.

Description

technical field [0001] The invention relates to the field of animal biomedical engineering, in particular to a preparation method of an orally porcine epidemic diarrhea virus subunit vaccine. Background technique [0002] Porcine epidemic diarrhea virus (PEDV) is a single-stranded, positive-sense RNA virus belonging to the order Nidovirales, the family Coronviridae, and the genus Coronavims. Infection with PEDV is one of the main causes of porcine viral diarrhea. The main features are severe enteritis, vomiting, watery diarrhea and weight loss. The high mortality rate of piglets makes PEDV infection a major loss in China's pig industry. factor. [0003] PEDV has not been effectively controlled since it was discovered until today, but has become a major virus affecting the pig industry all over the world, causing huge economic losses. The outbreak of PED in Asia caused extremely high mortality. Studies have shown that when PED broke out in Japan from 1993 to 1994, more than...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K47/36A61K47/12A61P31/14
Inventor 马静云磨健悦曾喜多孙宝丽毕英佐
Owner SOUTH CHINA AGRI UNIV
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