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Method capable of extracting bilirubin from bile by ultrasonic-microwave synergistic vacuum system

A technology of synergistic microwave and bilirubin, applied in the direction of organic chemistry, can solve the problems of increasing the difficulty of post-processing, affecting the purity of the target product, and long extraction time, so as to achieve product purity, high yield, and no pollution to the environment Effect

Inactive Publication Date: 2015-02-18
白宝功 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the preparation of bilirubin by ultrasonic method can overcome some defects in the traditional technology, it still has long extraction time, some bilirubin is oxidized during the ultrasonic process, and the extraction rate is low. Antioxidants need to be added during the preparation process, which increases the cost of post-treatment. Difficulty, affecting the purity of the target product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting bilirubin from bile with ultrasound and microwave vacuum system, comprising the following method steps: (1) Pretreatment: cut the animal gall with non-embroidered medical scissors to obtain 100 mL of juice, add 150 mL of petroleum ether at 30°C to 60°C , placed on an oscillator at room temperature for 15 minutes, then statically separated the bile for later use; (2) Extraction: At room temperature, place the bile pretreated in step (1) in an ultrasonic microwave extractor, ultrasonic power: 400W, ultrasonic frequency : 15kHz, microwave power: 500w, microwave frequency 2450MHz, vacuum degree: 0.065MPa, extraction time 5min. (3) Extraction: Add 25 mL of dimethylformamide to step (2), separate the bilirubin extracted in step (2) from bile, and extract 3 times.

Embodiment 2

[0022] A method for extracting bilirubin from bile with ultrasound and microwave vacuum system, comprising the following method steps: (1) Pretreatment: cut the animal gall with non-embroidered medical scissors to obtain 100 mL of juice, add 150 mL of petroleum ether at 30°C to 60°C , placed on an oscillator at room temperature for 30 minutes, then statically separated the bile for later use; (2) Extraction: At room temperature, place the bile pretreated in step (1) in an ultrasonic microwave extractor with ultrasonic power of 1200W and ultrasonic frequency of 28kHz , microwave power 900W, microwave frequency 2450MHz, vacuum degree 0.096MPa, extraction time 15min. (3) Extraction: Add 15 mL of dimethylacetamide to step (2), separate the bilirubin extracted in step (2) from bile, and extract 7 times.

Embodiment 3

[0024] A method for extracting bilirubin from bile with ultrasound and microwave vacuum system, comprising the following method steps: (1) Pretreatment: cut the animal gall with non-embroidered medical scissors to obtain 100 mL of juice, add 150 mL of petroleum ether at 30°C to 60°C , placed on an oscillator at room temperature for 20 minutes, then statically separated the bile for later use; (2) Extraction: At room temperature, place the bile pretreated in step (1) in an ultrasonic microwave extractor with an ultrasonic power of 500W and an ultrasonic frequency of 18kHz , microwave power 600w, microwave frequency 2450MHz, vacuum degree 0.075MPa, extraction time 6min. (3) Extraction: Add 20 mL of dichloromethane to step (2), separate the bilirubin extracted in step (2) from bile, and extract 5 times.

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Abstract

The invention relates to a bile processing method and in particular relates to a method for preparing bilirubin from bile in ultrasonic-microwave synergistic vacuum. The method comprises the following steps: (1) preprocessing; (2) extracting, namely putting the bile processed by the step (1) under the room-temperature condition in an ultrasonic-microwave extractor in which the ultrasonic power is 400W-1200W, the ultrasonic frequency is 15kHz-28kHz, the microwave power is 500W-900W, the microwave frequency is 2450MHz and the vacuum degree is 0.065MPa-0.096MPa, and extracting for 5min-15min; (3) extracting, namely adding 15mL-25mL of an extraction agent into the bile in the step (2), separating the bilirubin extracted by the step (2) from the bile and extracting for 3-7 times. By virtue of the method, the microwave electromagnetic field theory and the ultrasonic cavatition effect theory firstly make an organic combination; the advantages of the two theories are fully played; the two theories synergistically effect; the bilirubin can be quickly extracted from the bile without damage; compared with an ultrasonic extraction method, the yield is improved by 13%-20%.

Description

technical field [0001] The invention relates to a method for processing bile, in particular to a method for preparing bilirubin from bile under vacuum in conjunction with ultrasound and microwave. Background technique [0002] Bilirubin (BR) is a bile pigment extracted from animal bile. It is an important reagent in analytical chemistry and biochemical research and a valuable biochemical drug. It is also the main raw material of many drugs and has a wide range of uses in the medical field. Clinically, it has the effects of antipyretic, expectorant, sedative, anticonvulsant, antibacterial, antihypertensive and promoting hemoglobin regeneration, and it is effective against Japanese encephalitis virus and W 256 Cancer cells have an inhibitory effect. [0003] Bilirubin is mainly extracted from the bile of pigs, cattle, sheep and other animals or human bile drained from hospitals. At present, calcium salt method and hydrolysis method are mainly used in production to extract bil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D207/44
CPCC07D207/44
Inventor 项敬来
Owner 白宝功