Troponin diagnosis test paper strip for coupled immunomagnetic beads

Abstract

The invention relates to a troponin diagnosis test paper strip for coupled immunomagnetic beads. The structure of the troponin diagnosis test paper strip is that an NC (nitrocellulose) film is arranged between a combined pad and an absorption pad and the NC film is an area in which a blood sample to be detected is dropped. The troponin diagnosis test paper strip is characterized in that the immunomagnetic beads are accommodated in the NC film; the immunomagnetic beads are magnetic beads with carboxyl on the surfaces, and are antibody-coupled immunomagnetic beads formed after coupling with a monoclonal antibody in an activated buffer solution. In an optimal scheme, the using amount of the antibody-coupled immunomagnetic beads and the blood sample to be detected are as follows: 4-5 microliters of 16A11 antibody-coupled immunomagnetic beads, 4-5 microliters of 810 antibody-coupled immunomagnetic beads, 2.5-3.5 microliters of blood sample to be detected, and 142-152 microliters of mixed buffer solution. The troponin diagnosis test paper strip for coupled immunomagnetic beads disclosed by the invention overcomes the defects in the prior art, so that troponin can be simply and quickly detected, the detection result is more accurate, the application field is wider, and the utilization rate for antibody raw materials is higher.

Description

technical field [0001] The invention relates to a medical detection tool, in particular to a troponin diagnostic test strip coupled with immunomagnetic beads. Background technique [0002] Troponin is a protein molecule that regulates cardiac contractility and consists of three subunits, troponin C (TnC), troponin T (TnT) and troponin I (TnI). With the continuous development of the application of cardiac biomarkers, troponin has become the marker of choice for the diagnosis of myocardial infarction (MI). The development of rapid clinical test (POCT) of this marker can allow patients to receive timely and appropriate treatment. Taking Roche’s troponin T test card as an example, the critical value of cTnT is 0.1ng / ml, the detection range of the test card is 0.1-2ng / ml, and the detection time is 8-12 minutes. [0003] The immunological methods currently used for POCT diagnosis of cTnT and cTnI mainly include colloidal gold immunochromatography and fluorescence methods. Both ...

AI Technical Summary

Problems solved by technology

The supporting optical detector has high requirements on the wavelength range of the excitation light, while the emission power of the conventional excitation light is low, which leads to the high manufacturing cost of the whole set of detection products including the luminescent material and the detector, which limits the Mass production and popularization of materials

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