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Method for preparing corbicula fluminea juice having sobering-up function through ultrasonic assisted extraction and enzymolysis

An auxiliary extraction and ultrasonic technology, which is applied in the field of ultrasonic-assisted water extraction and preparation of clam juice by enzymolysis, can solve the problems of low content of active ingredients in clam juice, low absorption efficiency, long time consumption, etc., and achieves remarkable sobering effect, high absorption rate, The effect of short extraction times

Inactive Publication Date: 2015-02-25
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an ultrasonic-assisted extraction technology to solve the technical problems of low efficiency, high energy consumption, long time consumption, high cost, low active ingredient content of clam juice and low absorption efficiency in the technology of hot water extraction of clam juice. 1. Enzymatic hydrolysis of clam juice with sobering effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for temporary feeding and spit out sand, after the sand is spit out, pour them into the cleaning tank for cleaning, weigh the cleaned river clams into an ultrasonic extraction container, Add water according to the ratio of material to liquid 1:3, and under the conditions of ultrasonic power 500 W / Kg, frequency 20 KHZ, 90 ℃, ultrasonic assisted extraction for 5 minutes to obtain water extract; Filter the water extract to obtain the filtrate, adjust the pH value to 7.2, add 1500 U / mL clam juice compound protease (the composition of the compound protease is: trypsase:alkaline protease (Alcalase)=3:1 ), under the conditions of ultrasonic power 300 W, frequency 20 KHZ, 55 ℃, ultrasonic assisted enzyme hydrolysis for 7 min, and heating at 100 ℃ for 5 min to obtain enzyme hydrolyzate; polysulfone hollow fiber with a molecular weight cut-off of 5 kDa Ultrafiltration membrane,...

Embodiment 2

[0018] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for temporary feeding and spit out sand, after the sand is spit out, pour them into the cleaning tank for cleaning, weigh the cleaned river clams into an ultrasonic extraction container, Add water according to the ratio of material to liquid 1:2, and under the conditions of ultrasonic power 400 W / Kg, frequency 20 KHZ, 90 ℃, ultrasonic assisted extraction for 10 min to obtain water extract; Filter the water extract to obtain the filtrate, adjust the pH value to 7.4, add 1800 U / mL clam juice compound protease (the composition of the compound protease is: trypsase:alkaline protease (Alcalase)=3:1 ), under the conditions of ultrasonic power 400 W, frequency 20 KHZ, and 52 °C, ultrasonic-assisted enzyme hydrolysis for 8 min, and heating at 100 °C for 5 min to obtain enzyme hydrolyzate; polysulfone hollow fibers with a molecular weight cut-off of 5 kDa Ultrafiltration membr...

Embodiment 3

[0020] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for holding and spitting sand, after sand spitting, pour them into the cleaning tank for cleaning, weigh the cleaned river clams in an ultrasonic extraction container , add water according to the ratio of material to liquid 1:1, under the conditions of ultrasonic power 600 W / Kg, frequency 20 KHZ, 90 ℃, ultrasonic assisted extraction for 6 minutes, to obtain water extract; Filter the water extract to obtain the filtrate, adjust the pH value to 7.8, add 1600 U / mL clam juice compound protease (the composition of the compound protease is: trypsase:alkaline protease (Alcalase)=3: 1) Under the conditions of ultrasonic power 400 W, frequency 20 KHZ, and 50 °C, ultrasonic-assisted enzymatic hydrolysis for 8 min, and heating at 100 °C for 5 min to obtain enzymatic hydrolyzate; polysulfone hollow polysulfone with a molecular weight cut-off of 5 kDa Fibrous ultrafiltration membr...

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Abstract

The invention discloses a method for preparing a corbicula fluminea juice having a sobering-up function through ultrasonic assisted extraction and enzymolysis, wherein the method comprises the following steps: cultivating corbicula fluminea for short duration to spit sand, cleaning, adding water, and carrying out ultrasonic assisted extraction; carrying out vacuum suction filtration, regulating a pH value of the filtrate, adding a compound protease of trypsin and alkaline protease, and carrying out ultrasonic assisted two-protease collaborative enzymolysis; carrying out enzyme deactivation, carrying out separation concentration with an ultrafiltration membrane, and thus obtaining the corbicula fluminea juice having the sobering-up function. The differences between the method and a conventional hot water extraction method are that the ultrasonic effect is utilized for fully extracting water-soluble effective ingredients in corbicula fluminea, and ultrasonic assisted two-protease collaborative enzymolysis is utilized to obtain the corbicula fluminea juice being beneficial for absorption and having the sobering-up function; the corbicula fluminea juice concentrate obtained by the ultrafiltration membrane separation concentration technology has high effective ingredient content and has the significant sobering-up effect. The method has the advantages of simple process, high efficiency, time saving, energy saving, income increase and the like, and the prepared corbicula fluminea juice has the significant sobering-up function, and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for preparing clam juice assisted by ultrasonic water extraction and enzymatic hydrolysis, belonging to the field of aquatic product processing. Background technique [0002] River clam, also known as yellow clam, is a freshwater shellfish with fast growth, strong reproductive ability, large breeding output and extremely rich resources. River clams are rich in protein, glycogen, essential amino acids, taurine, multivitamins, calcium, phosphorus, iron, selenium and other minerals with special physiological functions, and also contain physiologically active substances such as active peptides, which have high Health effects. "Compendium of Materia Medica" records, "Clam, mainly cures appetizers, suppresses Danshi drug poison, removes violent heat, name and name, diuresis, relieves alcohol poisoning, and treats yellow eyes." [0003] Clam juice is delicious, and now concentrated clam juice seasoning has entered the marke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/221A23L27/10
CPCA23L33/40A23L17/40A23L27/10A23V2002/00
Inventor 朱小燕李相前顾姝妮
Owner HUAIYIN INSTITUTE OF TECHNOLOGY