A kind of preparation method of natural colorless biological anti-mold and antiseptic agent
A fungicide and biological technology, applied in the preparation of natural colorless biological anti-mold and anti-bacterial agents, and the field of preparation of anti-mold and anti-bacterial agents, can solve the problems of reducing yield, affecting decolorization effect, loss of active components and the like, Achieving the effect of low price, wide source and rich nutrition
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Embodiment 1
[0047] (1) Preparation of Lysobacter zymogenes C3 seed solution
[0048] 1) The formula of LB liquid medium is: based on the weight of 1000 grams of water, 10 grams of peptone, 5 grams of yeast extract, and 10 grams of NaCl.
[0049] 2) Preparation
[0050] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and culture it on a shaker at 230 r / min and 28°C for 40 hours to prepare Lysobacterium enzymogenes C3 seed liquid.
[0051] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent
[0052] 1) Based on the weight of 10,000 grams of water, the formula of the liquid medium is:
[0053] NaCl 50g
[0054] Bean cake powder 350g
[0055] 200g corn flour
[0056] CaCl 2 10 grams
[0057] 2) Preparation
[0058] Fill the liquid medium into a 5L fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was 80% ...
Embodiment 2
[0071] (1) Preparation of Lysobacter zymogenes C3 seed solution
[0072] 1) The formula of LB liquid medium is: based on the weight of 1000 grams of water, 10 grams of peptone, 5 grams of yeast extract, and 10 grams of NaCl.
[0073] 2) Preparation
[0074] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and place it on a shaker at 230 r / min and 30°C for 30 hours to prepare Lysobacterium enzymogenes C3 seed liquid.
[0075] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent
[0076] 1) Based on the weight of 10,000 grams of water, the formula of the liquid medium is:
[0077] NaCl 50g
[0078] Bean cake powder 150g
[0079] 500g corn flour
[0080] CaCl 2 10 grams
[0081] 2) Preparation
[0082] Fill the liquid medium into a 5L fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was 70% of ...
Embodiment 3
[0093] (1) Preparation of Lysobacter zymogenes C3 seed solution
[0094] 1) The formula of LB liquid medium is: based on the weight of 1000 parts of water, 10 parts of peptone, 5 parts of yeast extract, and 10 parts of NaCl.
[0095] 2) Preparation
[0096] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and culture it on a shaker at 200 r / min at 26°C for 38 hours to prepare Lysobacterium enzymogenes C3 seed liquid.
[0097] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent
[0098]1) Based on the weight of 10000 parts of water, the formula of the liquid medium is:
[0099] NaCl 100g
[0100] Bean cake powder 150g
[0101] 300g corn flour
[0102] CaCl 2 5 grams;
[0103] 2) Preparation
[0104] Fill the liquid culture medium into the fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was ...
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Abstract
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