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A kind of preparation method of natural colorless biological anti-mold and antiseptic agent

A fungicide and biological technology, applied in the preparation of natural colorless biological anti-mold and anti-bacterial agents, and the field of preparation of anti-mold and anti-bacterial agents, can solve the problems of reducing yield, affecting decolorization effect, loss of active components and the like, Achieving the effect of low price, wide source and rich nutrition

Active Publication Date: 2018-02-16
福州科力恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study also found that the decolorization adsorbent has defects such as ash content, pore capacity, micropore distribution, specific surface area and insufficient adsorption selectivity, which affect the decolorization effect, and the yield is reduced due to the serious loss of active components due to poor selectivity.

Method used

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  • A kind of preparation method of natural colorless biological anti-mold and antiseptic agent
  • A kind of preparation method of natural colorless biological anti-mold and antiseptic agent
  • A kind of preparation method of natural colorless biological anti-mold and antiseptic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of Lysobacter zymogenes C3 seed solution

[0048] 1) The formula of LB liquid medium is: based on the weight of 1000 grams of water, 10 grams of peptone, 5 grams of yeast extract, and 10 grams of NaCl.

[0049] 2) Preparation

[0050] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and culture it on a shaker at 230 r / min and 28°C for 40 hours to prepare Lysobacterium enzymogenes C3 seed liquid.

[0051] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent

[0052] 1) Based on the weight of 10,000 grams of water, the formula of the liquid medium is:

[0053] NaCl 50g

[0054] Bean cake powder 350g

[0055] 200g corn flour

[0056] CaCl 2 10 grams

[0057] 2) Preparation

[0058] Fill the liquid medium into a 5L fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was 80% ...

Embodiment 2

[0071] (1) Preparation of Lysobacter zymogenes C3 seed solution

[0072] 1) The formula of LB liquid medium is: based on the weight of 1000 grams of water, 10 grams of peptone, 5 grams of yeast extract, and 10 grams of NaCl.

[0073] 2) Preparation

[0074] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and place it on a shaker at 230 r / min and 30°C for 30 hours to prepare Lysobacterium enzymogenes C3 seed liquid.

[0075] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent

[0076] 1) Based on the weight of 10,000 grams of water, the formula of the liquid medium is:

[0077] NaCl 50g

[0078] Bean cake powder 150g

[0079] 500g corn flour

[0080] CaCl 2 10 grams

[0081] 2) Preparation

[0082] Fill the liquid medium into a 5L fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was 70% of ...

Embodiment 3

[0093] (1) Preparation of Lysobacter zymogenes C3 seed solution

[0094] 1) The formula of LB liquid medium is: based on the weight of 1000 parts of water, 10 parts of peptone, 5 parts of yeast extract, and 10 parts of NaCl.

[0095] 2) Preparation

[0096] Put the activated Lysobacterium enzymogenes C3 and a ring of seeds in a 500ml Erlenmeyer flask filled with 70ml LB liquid medium, and culture it on a shaker at 200 r / min at 26°C for 38 hours to prepare Lysobacterium enzymogenes C3 seed liquid.

[0097] (2) Preparation of highly active Lysobacterium enzymogenes C3 anti-mold and antiseptic agent

[0098]1) Based on the weight of 10000 parts of water, the formula of the liquid medium is:

[0099] NaCl 100g

[0100] Bean cake powder 150g

[0101] 300g corn flour

[0102] CaCl 2 5 grams;

[0103] 2) Preparation

[0104] Fill the liquid culture medium into the fermenter, and insert the seed liquid of Lysobacter zymogenes C3. The filling volume of the liquid medium was ...

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Abstract

The invention relates to a preparation method of a natural colorless biological mildew-proof anticorrosive bactericide. The preparation method comprises the following steps: firstly inoculating lysobacter enzymogenes C3 and a circle of seeds in a conical flask, and culturing on a shaking table to obtain a lysobacter enzymogenes C3 seed liquid; putting a liquid culture medium in a fermentation tank and then inoculating the lysobacter enzymogenes C3 seed liquid for culture to obtain a high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide; heating the high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide for 2-10 minutes at the temperature of 60 to 100 DEG C in order to perform thermal treatment; finally adding modified active carbon into the bactericide to perform a decoloration reaction to obtain the natural colorless biological mildew-proof anticorrosive bactericide, wherein the amount of the added modified active carbon is 0.5-3.0 percent of the weight of the high-activity lysobacter enzymogenes C3 mildew-proof anticorrosive bactericide which is in light brown. Due to acidic modification, an acidic oxygen-containing functional group on the surface of the active carbon is enabled to have polarity property, so the adsorption on compounds which are relatively high in polarity is enhanced, the adsorption capacity of the active carbon is improved, and the preferential adsorption of the active carbon on specific impurities like pigments is improved.

Description

technical field [0001] The invention relates to a preparation method of an anti-mildew and antiseptic agent, which belongs to the field of light industry, in particular to a preparation method of a natural colorless biological anti-mold and antiseptic agent. Background technique [0002] Bacteria and molds are widely distributed in nature, and there are various types, large numbers, and strong reproductive ability. They often cause considerable harm to humans, causing mildew and rot in bamboo, wood, food, clothing, flowers, paper cultural relics, and various equipment, or causing diseases of humans, animals, and crops. According to the statistics of the United Nations Food and Agriculture Organization (FAO), up to 25% of the world's agricultural products are polluted by molds every year, and their reproduction produces mycotoxins that threaten the safety of humans, animals and plants. There is an urgent need to find ways to prevent and control mold pollution. Biological ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01N3/00A01P1/00A01P3/00D21H21/36B27K3/52C12R1/01
CPCA01N3/00A01N63/10B27K3/52C12N1/20D21H21/36
Inventor 王明兹邹锦群陈必链吴钦缘沈月毛杜良成
Owner 福州科力恩生物科技有限公司
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