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Cordyceps sinensis strain spore separation, purification and rejuvenation method

A technology for purification and rejuvenation of Cordyceps sinensis, which is applied in the field of separation, purification and rejuvenation of spores of Cordyceps sinensis strains, can solve the problems of incomplete removal of miscellaneous bacteria, difficulty in obtaining spores, difficulty in disinfection, etc., and achieves strong anti-miscellaneous bacteria, large spores, and low cost Effect

Inactive Publication Date: 2015-03-11
CHONGQING ZONGYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Isolation of Cordyceps sinensis wild fungus and artificial cultivation of Cordyceps sinensis species tend to degenerate after several generations, and need to be purified and rejuvenated. However, it is difficult to sterilize with the separation of fruit bodies, and the mycelium is often killed or the miscellaneous bacteria cannot be completely removed. Difficult to achieve as spores are difficult to obtain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Separation, purification and rejuvenation of spores of wild Cordyceps sinensis

[0014] To separate, purify and rejuvenate the spores of wild Cordyceps sinensis, follow the steps below:

[0015] 1) Preparation of solid medium: The formula is: 120g potatoes, 12g glucose, 5g beef extract, KH 2 PO 4 0.5g, MgSO 4 0.25g, 10g agar; cook potatoes first, filter, add glucose, beef extract, KH 2 PO 4 , MgSO 4 , Agar, heat and boil to melt the agar completely, add distilled water to 500ml, under high pressure steam at 120℃, 30min, stir evenly and set the slope, set aside;

[0016] 2) Choose wild Cordyceps species with fast maturation of spores. The collection of wild Cordyceps species should be around May, preferably in Naqu, Tibet, Yushu, Qinghai and other places. Choose fresh wild species with strong and well-developed spores and fast spore formation; use 0.1% The mercury-liquid liquid disinfects the surface of the body of the bacteria, and then cleans it with sterile water ...

Embodiment 2

[0019] Example 2 Separation, purification and rejuvenation of artificially cultivated Cordyceps spores

[0020] To separate, purify and rejuvenate the artificially cultivated Cordyceps spores, follow the steps below:

[0021] 1) Preparation of solid medium: The formula is: 130g potatoes, 13g glucose, 6g beef extract, KH 2 PO 4 0.6g, MgSO 4 0.3g, 11g agar; first cook potatoes, filter, add glucose, beef extract, KH 2 PO 4 , MgSO 4 , Agar, heat and boil to melt the agar completely, add distilled water to 500ml, under high pressure steam at 120℃, 30min, stir evenly and set the slope, set aside;

[0022] 2) Select artificially cultivated Cordyceps strains with dense, sturdy and well-developed pedestals, bright-colored spores that mature quickly, and sterilize the body of the worms with 0.1% mercury solution, and then sterile the pedestals together Water cleaning

[0023] 3) Hammer a small stainless steel fish hook into the center of the lower end of the test tube stopper to expose the sma...

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PUM

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Abstract

The invention provides a cordyceps sinensis strain spore separation, purification and rejuvenation method. The cordyceps sinensis strain spore separation, purification and rejuvenation method comprises the following steps of 1 preparing a solid culture medium, namely thoroughly cooling and filtering potatoes 100 g-140 g, adding glucose10 g-14 g, beef extract 4 g-6 g, KH2PO4 0.4 g-0.6 g, MgSO4 0.2g-0.3 g and agar 8 g-12g, performing heating and boiling till the agar is completely molten, adding distilled water, performing high-pressure steam disinfection and installing an oblique plane; 2 selecting cordyceps sinensis strains with ripening spores, using 0.1% of corrosive sublimate solution to perform disinfection and using sterile water for washing; 3 hamming a small hook at the lower end of a test tube plug, cutting a strain seed seat under the aseptic condition, inversely hanging the strain seed seat on the small hook, and plugging the test tube plug into an oblique plane test tube; 4 keeping the oblique plane test tube in a dark place at the temperature of 22-24 DEG C to perform cultivation for 3-4 days, then performing cultivation at the temperature of 20-22 DEG C for 4-5 days, performing cultivation under the natural light condition at the temperature of 18-22 DEG C for 24 hours and selecting bright-yellow microbe-free strains with developed hyphae.

Description

Technical field [0001] The invention belongs to the field of biology and relates to a method for separating, purifying and rejuvenating cordyceps fungus spores. Background technique [0002] Cordyceps sinensis is a complex of the sub-base and larval body of the ergot cordyceps parasitic on the larva of the Hepialidae. There are two kinds of wild and artificially cultivated. The active ingredients that exert effects on the human body are cordycepin, cordycepic acid, cordyceps polysaccharide, and superoxide dismutase. It has many functions such as invigorating the kidney and lungs, stopping bleeding and resolving phlegm, fighting cancer, improving immunity, fighting fatigue, and protecting lungs, kidneys, and liver. It is well received by the majority of people and is called the soft gold in health care products. [0003] Isolation of Cordyceps wild fungi and artificial cultivation of Cordyceps for several generations are prone to degradation, and need to be purified and rejuvenat...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCC12N3/00
Inventor 梁金权
Owner CHONGQING ZONGYI PHARMA