New Lentinus tuber-regium strain, and culture method and application thereof

A technology of Pleurotus sclerotiorum and a cultivation method, which is applied to a new strain of Pleurotus sclerotiorum and its cultivation and application fields, can solve problems such as no research reports on strains, and achieve good social benefits and enrich the effects of strain varieties.

Active Publication Date: 2015-03-11
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application is a new fungus found and isolated from rotting wood in the broad-leaved f...

Method used

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Embodiment 1

[0021] A new strain of Pleurotus sclerotiorum, isolated from rotting wood in the broad-leaved forest of Maofeng Mountain in Guangzhou, was identified as a new strain of Pleurotus sclerotiorum by morphology and molecular biology. The original strain was obtained by tissue isolation of the fruiting body, named Sclerotia Pleurotus (Lentinus tuber-regium) GIM-L-001 was deposited in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Bayi Road, Hongshan District, Wuhan City) on August 26, 2014, and the preservation number is CCTCC NO. M2014392.

[0022] The characteristics of the Pleurotus sclerotiorum species CCTCC M2014392 are: fast growth, mycelia can cover a test tube (18×180mm) in about 12 days, mycelium growth temperature is 15-40°C, and the optimum growth temperature is 28-30°C. Mycelial growth pH 5.5-7, the optimum pH 6.5.

Embodiment 2

[0024] The preparation method of the cultivated species of Pleurotus sclerotiorum species CCTCC M2014392:

[0025] (1) Production of parent species: collect the sclerotia Pleurotus CCTCC NO.M2014392 isolated from the tissue, aseptically connect the strain to the slope of the improved PDA, and place it in a 28°C incubator for constant temperature cultivation until the mycelia cover the slope. For the mother species, this process takes about 12 days, and the mycelium growth is as follows: the mycelium is white, dense, and does not form rod-shaped structures and fruiting bodies;

[0026] Wherein the formula of the improved PDA slant is calculated by mass percentage as 20% of potatoes, 2% of glucose, 2% of agar, 0.15% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate, trace amounts of vitamin B1, 1% of acid hydrolyzed casein, and the rest are water;

[0027] (2) Making the original seed: aseptically insert the mother seed into the original seed bottle, place it in a 2...

Embodiment 3

[0032] The cultivation method of Pleurotus sclerotiorum species CCTCC M2014392:

[0033] Mix the cultivation material according to the proportion and put it into a 17*35cm polyethylene corner bag to obtain a cultivation bag. Use a puncher to punch a hole of 10cm, put on a plastic collar and cover, and use a high-pressure steam sterilizer, 1.5kg / cm 2 Sterilize for 2 hours. After the sterilization is completed, wait for the cultivation material to cool below 30°C, insert the cultivars, transfer to 28°C-30°C for indoor cultivation, and cultivate for about 50 days. The hyphae will cover the cultivation bag, remove the plastic collar and Cover, adjust the humidity to 88%-85%, light 100-200lux, and ventilate in time. Fruiting bodies can be formed in about 30 days and picked.

[0034] The raw material composition of the cultivation material is 78-80% wood chips, 18-20% bran, CaCO 3 1-2%, water content 70-75%.

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Abstract

The invention relates to a new Lentinus tuber-regium strain, and a culture method and application thereof. The new Lentinus tuber-regium strain is Lentinus tuber-regium GIM-L-001, and the collection number is CCTCC NO.M2014392. The culture method of the Lentinus tuber-regium CCTCC M2014392 comprises the following steps: inoculating the Lentinus tuber-regium CCTCC NO.M2014392 culture strain in a culture bag, culturing, and managing collection. The invention provides a new Lentinus tuber-regium strain from rottenwood in broadleaf forest in Maofeng Mountain in Guangzhou, and a corresponding artificial culture method and corresponding application thereof. The new Lentinus tuber-regium strain has antibacterial activity, enriches the strain species, and has favorable social benefit.

Description

technical field [0001] The invention relates to a new strain of Pleurotus sclerotiorum and its cultivation method and application. Background technique [0002] Pleurotus sclerotium, also known as tiger milk mushroom, nuclear ear mushroom, and Pleurotus tuckahoe, is called Nanyang tuckahoe in Japan. It is a medicinal and edible mushroom. It is a typical high-temperature edible and medicinal fungus. According to the latest classification system, its Latin scientific name is Lentinus tuber -regium (Fr.) Fr., belonging to Basidiomycota (Basidiomycota), Agaricomycotina (Agaricomycotina), Agaricomycetes (Agaricaceae), Polyporales (Polypores), Polyporaceae (Polyporaceae), Lentinus (Mushroom genus), is a fungus with sclerotia, which is different from Poria cocos (inedible fruiting bodies) and Polyporus (inedible sclerotias), both of which are edible. Pleurotus sclerotium is a tropical fungus, mainly distributed in tropical and subtropical regions, Yunnan, Hainan, Guangdong and oth...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04A61K36/07A61P31/00C12R1/645
CPCA61K36/07A01G18/00C12N1/145C12R2001/645
Inventor 刘远超胡惠萍吴丽霞曹仁润黄龙花谭武平冯德龙
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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