Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing high-purity chlorogenic acid from jerusalem artichoke leaves

A chlorogenic acid and high-purity technology is applied in the field of preparing high-purity chlorogenic acid, which can solve the problems of low research depth and achieve the effects of continuous and simple operation, efficient separation and simple operation method.

Active Publication Date: 2015-03-18
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with domestic research on extracting chlorogenic acid from traditional high-priced medicinal resources such as honeysuckle, the depth of research on extracting and purifying chlorogenic acid from Jerusalem artichoke is still relatively low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high-purity chlorogenic acid from jerusalem artichoke leaves
  • Method for preparing high-purity chlorogenic acid from jerusalem artichoke leaves
  • Method for preparing high-purity chlorogenic acid from jerusalem artichoke leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 150g of Jerusalem artichoke leaf meal and put it into an extractor, add 750mL of 20% methanol aqueous solution, heat and reflux at 100°C for extraction twice, each time for 1h. Filter and combine the extracts. Concentrate the extract under reduced pressure to 10% of the original volume fraction, first pass through the D101 macroporous resin column at a flow rate of 5BV / h to remove impurities such as flavonoids, tannins, sugars, and the effluent directly at a flow rate of 5BV / h Put on the ADS-17 macroporous resin column, after the dynamic adsorption is saturated, wash the ADS-17 macroporous resin column with deionized water at a flow rate of 3BV / h until the effluent is colorless, and then use 100% ethanol Elute at a flow rate of 5BV / h, collect the ethanol eluate, concentrate under reduced pressure to a paste, and then dissolve the extract in water according to the ratio of material to liquid 1:5 (g / mL). The aqueous solution is applied to the polyamide column at a f...

Embodiment 2

[0030] Put 600g of Jerusalem artichoke leaf coarse powder into the extractor, add 3500mL methanol aqueous solution with a volume fraction of 40%, and heat and reflux at 80°C for extraction twice, each time for 2h. Filter and combine the extracts. Concentrate the extract under reduced pressure to 10% of the original volume fraction, first pass through the AB-8 macroporous resin column at a flow rate of 4BV / h to remove impurities such as flavonoids, tannins, sugars, and the effluent directly at 4BV / h The flow rate of the S-8 macroporous resin column is passed through the S-8 macroporous resin column. After the dynamic adsorption is saturated, the S-8 macroporous resin column is rinsed with deionized water at a flow rate of 5BV / h until the effluent is colorless, and then the volume fraction is 80%. The ethanol was eluted at a flow rate of 8BV / h, the ethanol eluate was collected, concentrated under reduced pressure to a paste, and then the extract and water were dissolved in water...

Embodiment 3

[0032] Put 1500g of Jerusalem artichoke leaf coarse powder into the extractor, add 15000mL methanol aqueous solution with a volume fraction of 60%, and heat and reflux at 70°C for extraction twice, each time for 3h. Filter and combine the extracts. Concentrate the extract under reduced pressure to 10% of the original volume fraction, first pass through the D101 macroporous resin column at a flow rate of 2BV / h to remove flavonoids, tannins, sugars and other impurities, and the effluent directly at a flow rate of 3BV / h Pass through the X-5 macroporous resin column, after the dynamic adsorption is saturated, wash the X-5 macroporous resin column with deionized water at a flow rate of 8BV / h until the effluent is colorless, and then use 60% ethanol Elute at a flow rate of 7BV / h, collect the ethanol eluate, concentrate under reduced pressure to a paste, and then dissolve the extract in water according to the ratio of material to liquid 1:5 (g / mL). The aqueous solution is applied to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing high-purity chlorogenic acid from jerusalem artichoke leaves. The method comprises the following steps of: heating jerusalem artichoke leaves which serve as raw materials to carry out reflux or ultrasonic extraction, carrying out solid-liquid separation, concentrating at reduced pressure to obtain a crude chlorogenic acid concentrate, removing impurities of the crude chlorogenic acid concentrate by a macroporous resin column A, directly adding an efflux into a macroporous resin column B, dynamically adsorbing impurities in the efflux until the efflux is saturated, adding an impurity washing solution into the efflux, washing the efflux to remove impurities until the efflux becomes colorless, eluting the macroporous resin column B with an eluting solution until the detection by the HPLC (high performance liquid chromatography) shows that no chlorogenic acid flows out, collecting the eluting solution, concentrating the eluting solution at reduced pressure, adding the concentrated eluting solution into a polyamide column, adsorbing impurities, separating impurities, eluting the eluting solution, refining, and spray-drying to obtain chlorogenic acid with high purity of more than 98%. The method provided by the invention is simple in process, is convenient to operate, is relatively low in overall cost and is suitable for industrial production, solvents can be recycled, and macroporous resin and polyamide can be used repeatedly.

Description

technical field [0001] The invention relates to a method for preparing high-purity chlorogenic acid, in particular to a method for preparing high-purity chlorogenic acid from Jerusalem artichoke leaves. Background technique [0002] Fresh Jerusalem artichoke leaves contain a large amount of bioactive substances. Yuan Xiaoyan et al. (2008) reported that chlorogenic acid is an important bioactive component of the plant. The content of chlorogenic acid in fresh Jerusalem artichoke leaves is about 0.5%-5.0%, which is not much different from the content of chlorogenic acid in high-priced medicinal resources such as honeysuckle (1.0%-5.9%) and Eucommia (2.0%-5.0%). Moreover, Jerusalem artichoke is easy to plant and manage, has strong environmental adaptability, and has a positive role in promoting the improvement of the natural environment. Therefore, extracting chlorogenic acid from Jerusalem artichoke leaves has higher economic and environmental benefits. [0003] The raw mate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56
CPCC07C67/56C07C69/732
Inventor 夏传海孙鹏程方圣涛
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com