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Optimization method of calcified sodium alginate immobilization enzyme

A technology of sodium alginate and optimization method, which is applied in directions such as being fixed on/in an organic carrier, can solve the problems of polluted reaction system, unstable combination of sodium alginate and divalent cations, and unrecyclable enzymes.

Inactive Publication Date: 2015-03-18
杨晓曦
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Problems solved by technology

[0003] However, the combination of sodium alginate and divalent cations is not stable, and the gel particles of calcified sodium alginate-immobilized enzymes will be broken after 5-8 cycles, so that the enzymes cannot be recycled and the reaction system will be polluted.

Method used

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  • Optimization method of calcified sodium alginate immobilization enzyme
  • Optimization method of calcified sodium alginate immobilization enzyme

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Embodiment

[0016] The reaction selected in this example is the hydrolysis reaction of p-nitrophenol butyrate catalyzed by standardized ester hydrolase. The reaction has the advantages of rapid response, sensitivity, high yield, etc., and can quickly detect the optimization effect of the method of the present invention.

[0017] Get 0.02 g of the calcified sodium alginate gel beads (2) immobilized with the enzyme (1) prepared after freeze-drying and place them in a 10 ml supercritical carbon dioxide autoclave (4) and add 2 ml of deionized water to make the condensed The volume ratio of glue pellets to water is 1:2. Seal the reaction vessel, inject 10ml of supercritical carbon dioxide, keep the temperature of the system at 35°C and the pressure at 7.5MPa, and stir for 2 hours to react.

[0018] Take out the processed calcified sodium alginate-immobilized enzyme gel pellet (3) and put it into a 20ml beaker, add 3ml of deionized water, and then take 0.06mmol p-nitrophenol butyrate and drop ...

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Abstract

The invention discloses an optimization method of a calcified sodium alginate immobilization enzyme. The optimization method of the calcified sodium alginate immobilization enzyme is characterized by processing gel balls of the calcified sodium alginate immobilization enzyme by using supercritical carbon dioxide, wherein the carbon dioxide in the supercritical state is capable of transmitting a sodium alginate gel shell by virtue of the special fluid property, and is combined with calcium ions in the calcified sodium alginate gel to form nano spherical calcium carbonate particles which are adhered to a shell of a sodium alginate immobilization enzyme gel system, so that the strength of the gel shell is greatly improved; the cyclic utilization times of the immobilization enzyme system are increased. By virtue of the optimization method, the utilization times of the enzyme are increased; the cost is saved; no harmful substance is produced in the operation process; the optimization method is easy to operate, has a resource-saving effect and is environmentally friendly.

Description

technical field [0001] The invention relates to an optimized method for immobilizing enzymes with calcified sodium alginate. Background technique [0002] With the development of biotechnology, enzymatic reactions are increasingly used in industrial and pharmaceutical production. However, the most important element in enzymatic reactions—biological enzymes—has the disadvantages of being difficult to preserve and recycle. Therefore, scientists have developed various polymer-immobilized enzyme-immobilized methods to solve this problem, among which calcified sodium alginate-immobilized enzyme-immobilized method is particularly simple and easy to implement and is widely used. Sodium alginate is a natural polymer, mild and non-toxic, soluble in water at room temperature, and can combine with divalent cations to form a gel. The method of calcifying sodium alginate to immobilize the enzyme is to mix sodium alginate and enzyme and dissolve it in water, add the liquid dropwise to t...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04
Inventor 杨晓曦
Owner 杨晓曦
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