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Preparation method of organic/inorganic hybridized magnetic nano flower

A magnetic nano and inorganic technology, applied in the direction of magnetic materials, magnetic objects, nanotechnology, etc., can solve problems that have not yet been seen, and achieve high promotion and application value, rapid recovery and reuse effects

Active Publication Date: 2015-03-25
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the performance, magnetic composite microspheres with high specific surface area have become a research hotspot, but most of them are coated with inorganic mesoporous layers on the surface of magnetic inorganic particles or embedded in the framework of porous polymer microspheres (ZL 200910021833.5) Realized, CN201010120183.2 reported a water-soluble rock-salt type CoO nano-flower magnetic particle, but no reports of organic / inorganic hybrid magnetic composite microspheres similar to inorganic nano-flower materials

Method used

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  • Preparation method of organic/inorganic hybridized magnetic nano flower
  • Preparation method of organic/inorganic hybridized magnetic nano flower

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Bovine serum albumin / Zn 3 (PO 4 ) 2 / Fe 3 o 4 Preparation of hybrid magnetic nanoflowers

[0020] Will Fe 3 o 4 The particles are uniformly dispersed in water to obtain a mass fraction of 0.05-0.15% Fe 3 o 4 Add the particle water dispersion into a three-necked flask with mechanical stirring; prepare an aqueous solution of zinc acetate with a concentration of 0.2-0.5mol / L, add it into the three-necked flask, and stir mechanically for 0.5-3h. Then add 0.2 ~ 1g / L bovine serum albumin solution, the solvent is phosphate buffer solution, in which Fe 3 o 4 The volume ratio of the particle aqueous dispersion, the bovine serum albumin solution and the zinc acetate aqueous solution is 10-25:10-25:1. Under mechanical stirring, react at room temperature for 2 to 8 hours, then go through magnetic field separation, washing with water, and freeze drying to obtain bovine serum albumin / Zn 3 (PO 4 ) 2 / Fe 3 o 4 Hybrid magnetic nanoflowers.

Embodiment 2

[0021] Embodiment 2: bovine serum albumin / Zn 3 (PO 4 ) 2 / Fe 2 o 3 Preparation of hybrid magnetic nanoflowers

[0022] Will Fe 2 o 3 The particles are uniformly dispersed in water to obtain a mass fraction of 0.05-0.15% Fe 2 o 3 Add the particle water dispersion into a three-necked flask with mechanical stirring; prepare a zinc chloride aqueous solution with a concentration of 0.2-0.5mol / L, add it into the three-necked flask, and stir mechanically for 0.5-3h. Then add 0.2~1g / L bovine serum albumin solution, its solvent is phosphate buffer solution (pH=7.4), in which Fe 2 o 3 The volume ratio of the particle aqueous dispersion, the bovine serum albumin solution and the zinc chloride aqueous solution is 10-25:10-25:1. Under mechanical stirring, react at room temperature for 2 to 8 hours, then go through magnetic field separation, washing with water, and freeze drying to obtain bovine serum albumin / Zn 3 (PO 4 ) 2 / Fe 2 o 3 Hybrid magnetic nanoflowers.

Embodiment 3

[0023] Embodiment 3: Papain / Zn 3 (PO 4 ) 2 / Fe 3 o 4 Preparation of hybrid magnetic nanoflowers

[0024] Will Fe 3 o 4 The particles are uniformly dispersed in water to obtain a mass fraction of 0.05-0.15% Fe 3 o 4 Add the particle water dispersion into a three-necked flask with mechanical stirring; prepare a zinc nitrate aqueous solution with a concentration of 0.2-0.5mol / L, add it into the three-necked flask, and stir mechanically for 0.5-3h. Then add 0.2~1g / L papain solution, its solvent is a phosphate buffered saline solution (pH=7.2), wherein Fe 3 o 4 The volume ratio of the particle aqueous dispersion, the papain solution and the zinc nitrate aqueous solution is 10-25:10-25:1. Under mechanical stirring, after reacting at room temperature for 2 to 8 hours, the papain / Zn 3 (PO 4 ) 2 / Fe 3 o 4 Hybrid magnetic nanoflowers.

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Abstract

The invention relates to a preparation method of an organic / inorganic hybridized magnetic nano flower. The preparation method is technically characterized in that surface amino or carboxyl functionalized magnetic nano-grade or sub-micron-grade particles are used as a magnetic source, and the organic / inorganic hybridized magnetic nano flower formed by biological macromolecules (such as protein and enzymes) and zinc phosphate (Zn3(PO4)2) is used as a main framework; and coordination interaction between Zn<2+> and amino or carboxyl carried by the surfaces of the magnetic particles or the biological macromolecules is utilized by the compounding of the magnetic particles and the organic / inorganic hybridized magnetic nano flower, and the hybridized compounding of the biological macromolecules and (Zn3(PO4)2). The prepared organic / inorganic hybridized magnetic nano flower has a relatively high popularization and application value in the fields of adsorption separation, catalytic synthesis and the like according to the different biological macromolecules; and meanwhile, magnetic components are introduced so that materials can be rapidly recycled and reutilized.

Description

technical field [0001] The invention relates to a method for preparing organic / inorganic hybrid magnetic nanoflowers, in particular to biomacromolecules (proteins, enzymes, etc.), magnetic nanoscale or submicron particles, zinc phosphate (Zn 3 (PO 4 ) 2 ) A simple one-step composite of three materials to prepare organic / inorganic hybrid magnetic nanoflowers. Background technique [0002] Organic / inorganic hybrid magnetic composite microspheres integrate organic matter and magnetic inorganic particles, so they can be quickly separated and enriched under a magnetic field, and at the same time, they are easy to modify the surface for functionalization, so they are widely used in biochemistry, medicine, environment, detection , sensing and other fields have extensive and important applications. Among them, the magnetic inorganic particles are generally made of iron tetraoxide (Fe 3 o 4 ), ferric oxide (Fe 2 o 3 ), organic components can be various polymers (ZL201010202232...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02H01F1/00B82Y40/00B82Y30/00
CPCB01J13/02B82Y30/00B82Y40/00H01F1/00
Inventor 张宝亮张秋禹张和鹏田雷陈欣李欢王慎强
Owner NORTHWESTERN POLYTECHNICAL UNIV