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Method for extracting cordyceps polysaccharide

A technology of Cordyceps polysaccharide and extraction method, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as reducing the extraction rate of Cordyceps polysaccharide, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2015-03-25
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasonic extraction of polysaccharides has the characteristics of sufficient extraction and high extraction efficiency. However, hydrogen peroxide is generated during the process of ultrasonically disrupting cells. Hydrogen peroxide is oxidizing and will undergo redox reactions with reducing Cordyceps polysaccharides, thereby reducing Extraction rate of Cordyceps polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of Cordyceps culture medium

[0023] Cordyceps needs to be cultivated on medium, and three kinds of medium are used in this experiment: slant medium, seed medium and fermentation medium. The specific formulations of the three media are as follows:

[0024] Slant medium Potato 200g, glucose 20g, beef extract 10g, silkworm chrysalis powder 10g, peptone 10g, KH2PO4 3g, MgSO4 7H2O 1.5g, vitamin B1 10mg, agar 2.0, water 1000ml, pH7-8.

[0025] Seed medium Potato 200g, glucose 20g, beef extract 10g, peptone 10g, KH2PO4 3g, MgSO4·7H2O 1.5g, vitamin B1 10mg, water 1000ml, pH7-8.

[0026] Fermentation medium Potato 200 g, sucrose 10 g, peptone 10 g, soybean powder 10 g, KH2PO4 3 g, MgSO4·7H2O 1.5 g, vitamin B1 10 mg, water 1000 ml.

[0027] (2) Cordyceps culture

[0028] The Cordyceps fungus used in this experiment is Cordyceps sinensis-Hirsutella sinensis, which was purchased from the Institute of Microbiology, Chinese Academy of Sciences and stored in th...

Embodiment 2

[0037] (1) Preparation of Cordyceps culture medium

[0038]Cordyceps needs to be cultivated on medium, and three kinds of medium are used in this experiment: slant medium, seed medium and fermentation medium. The specific formulations of the three media are as follows:

[0039] Slant medium Potato 200g, glucose 20g, beef extract 10g, silkworm chrysalis powder 10g, peptone 10g, KH2PO4 3g, MgSO4 7H2O 1.5g, vitamin B1 10mg, agar 2.0, water 1000ml, pH7-8.

[0040] Seed medium Potato 200g, glucose 20g, beef extract 10g, peptone 10g, KH2PO4 3g, MgSO4·7H2O 1.5g, vitamin B1 10mg, water 1000ml, pH7-8.

[0041] Fermentation medium Potato 200 g, sucrose 10 g, peptone 10 g, soybean powder 10 g, KH2PO4 3 g, MgSO4·7H2O 1.5 g, vitamin B1 10 mg, water 1000 ml.

[0042] (2) Cordyceps culture

[0043] The Cordyceps fungus used in this experiment is Cordyceps sinensis-Hirsutella sinensis, which was purchased from the Institute of Microbiology, Chinese Academy of Sciences and stored in the Key...

Embodiment 3

[0052] (1) Preparation of Cordyceps culture medium

[0053] Cordyceps needs to be cultivated on medium, and three kinds of medium are used in this experiment: slant medium, seed medium and fermentation medium. The specific formulations of the three media are as follows:

[0054] Slant medium Potato 200g, glucose 20g, beef extract 10g, silkworm chrysalis powder 10g, peptone 10g, KH2PO4 3g, MgSO4 7H2O 1.5g, vitamin B1 10mg, agar 2.0, water 1000ml, pH7-8.

[0055] Seed medium Potato 200g, glucose 20g, beef extract 10g, peptone 10g, KH2PO4 3g, MgSO4·7H2O 1.5g, vitamin B1 10mg, water 1000ml, pH7-8.

[0056] Fermentation medium Potato 200 g, sucrose 10 g, peptone 10 g, soybean powder 10 g, KH2PO4 3 g, MgSO4·7H2O 1.5 g, vitamin B1 10 mg, water 1000 ml.

[0057] (2) Cordyceps culture

[0058] The Cordyceps fungus used in this experiment is Cordyceps sinensis-Hirsutella sinensis, which was purchased from the Institute of Microbiology, Chinese Academy of Sciences and stored in ...

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Abstract

The invention provides a method for extracting a cordyceps polysaccharide and relates to a method for extracting a biological polysaccharide. The method comprises cordyceps taishanensis cultivation and cordyceps polysaccharide extraction; the cordyceps taishanensis is cordyceps sinensis-hirsutellasinensis which is purchased from Institute of Microbiology of Chinese Academy of Sciences and preserved at a low temperature in the key laboratory of Shenyang University; mycelia are separated from a culture solution, an extracting solution with the cordyceps taishanensis powder is put under the probe of an ultrasonic disruption instrument, a knob is turned for disruption, the obtained precipitate is collected, a small amount of absolute ethyl alcohol, ethyl ether and acetone are orderly added for washing the precipitate, the precipitate is freeze-dried at -25 DEG C and resolved at 35 DEG C till constant mass, and then the cordyceps polysaccharide is obtained. The method for extracting the cordyceps polysaccharide has the advantages that the cordyceps polysaccharide is prevented from being reduced by hydrogen peroxide generated in the ultrasonic disruption process, the advantages of the ultrasonic disruption method are remained and the shortages of the ultrasonic disruption method are covered, and the extraction rate of the cordyceps polysaccharide is obviously increased.

Description

technical field [0001] The invention relates to a method for extracting biological polysaccharides, in particular to a method for extracting cordyceps polysaccharides. Background technique [0002] At present, with the continuous improvement of people's living standards, polysaccharides from Cordyceps sinensis have gradually entered people's lives. Cordyceps polysaccharide is widely extracted and used in many fields as the main medicinal ingredient. Ultrasonic extraction of polysaccharides has the characteristics of sufficient extraction and high extraction efficiency. However, hydrogen peroxide is generated during the process of ultrasonically disrupting cells. Hydrogen peroxide is oxidizing and will undergo redox reactions with reducing Cordyceps polysaccharides, thereby reducing Extraction rate of Cordyceps polysaccharide. In the present invention, on the basis of retaining the advantages of the ultrasonic extraction method, a certain concentration of potassium iodi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C08B37/00C12R1/645
CPCC12N1/14C08B37/00
Inventor 杨绍斌赵莹姜帅章
Owner SHENYANG UNIV
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