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Bacillus thuringiensis strain for efficient degradation of fly larvae protein

A technology for degrading fly maggot protein and Bacillus aureus, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of low nutrition utilization of fly maggot protein, achieve the effect of increasing content, broad development prospects, and improving nutritional value

Inactive Publication Date: 2015-03-25
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of fly maggot protein efficient degrading bacterial strain, be used for the degradation of fly maggot protein, thereby develop a kind of new useful protein resource, solve the problem that the nutrient utilization of fly maggot protein is low at present

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  • Bacillus thuringiensis strain for efficient degradation of fly larvae protein

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Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1 efficient bacterial strain is isolated

[0015] Take 1 gram of fly maggot repellent samples that died in the natural environment, add 100mL sterile saline, shake for 10 minutes, let stand for 5 minutes, spread the supernatant on the screening plate medium after 10-fold dilution, and grow at 37°C; The composition of the screening medium for fly maggot protein degrading strains is: 2.0g fly maggot protein powder, 0.5g sodium chloride, 2.0g agar powder, 100mL water, pH value 7.0, sterilized at 121°C for 30min.

[0016] The preparation method of culture medium of the present invention is as follows:

[0017] Accurately weigh 2.0g of fly maggot protein powder, add 100mL of water, heat to dissolve the protein, add 0.5g of sodium chloride, 2.0g of agar powder, adjust the pH value, and sterilize at 121°C for 30min.

[0018] The specific implementation of the screening of fly maggot protein degrading strains is as follows:

[0019] 1. Preliminary screening: After ...

Embodiment 2

[0038] The specific enzymatic property research of embodiment 2 fly maggot protein degrading enzyme is as follows:

[0039] The source of the enzyme: the fly maggot protein degrading strain was inoculated in the basic fermentation medium (3.0% bran, 1.0% peptone, 0.5% NaCl, natural pH, sterilized at 121°C for 30min), and cultivated at 36°C with a rotation speed of 180r / min for 48 hours, the culture solution was centrifuged at 4000r / min for 10min, and the supernatant was the fly maggot protein degradation enzyme solution.

[0040] (1) The optimum temperature of the enzyme: measure the activity of the degrading enzyme at different temperatures (35, 40, 45, 50, 55, 60°C), and the temperature at which the enzyme activity is the highest is the optimum temperature of the enzyme

[0041] (2) Stability of the enzyme to temperature: The degrading enzyme is incubated at different temperatures (35, 40, 45, 50, 55, 60°C) for 1 hour, and then the activity of the enzyme is measured, so as t...

Embodiment 3

[0046] Embodiment 3 fly maggot protein biodegradation effect:

[0047] Medium formula: glucose 2.0g, fly maggot protein 2.0g, NaCl 0.5g; water 100mL;

[0048] 1) Weigh each component according to the composition of the medium, and dissolve it with tap water;

[0049] 2) Put the prepared medium into the Erlenmeyer flask, stir and mix, sterilize at 121°C for 30 minutes, and cool;

[0050] 3) Inoculate the fly maggot protein-degrading bacteria and cultivate for 48 hours;

[0051] 4) Measure the amount of amino acid produced in the supernatant after the culture medium is 4000r / min, so as to determine its degradation degree.

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Abstract

The invention relates to a bacillus thuringiensis strain KF228911 for efficient degradation of fly larvae protein, and belongs to the technical field of agricultural microorganisms. The strain is classified and named as bacillus thuringiensis serovar thuringienesis which is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.9789 on October 17, 2014. The strain is inoculated to a seed culture medium, and is cultivated under 30 DEG C at 150r / min for 28hr, so that a KF228911 liquid culture is obtained. The strain can generate a transparent hydrolysis ring on a fly larvae protein powder culture medium and the fly larvae protein is degraded in a fly larvae protein fermentation medium, and the content of generated soluble amino acid can reach 1.744mg / mL. The strain disclosed by the invention can be used for degrading the fly larvae protein by virtue of microorganism fermentation, and is suitable for the production of high-quality protein feed. The strain is applicable to the development and utilization of fly larvae protein resource, and in particular applicable to transformation of the fly larvae protein into feed, so that the nutritive value of the fly larvae protein is improved.

Description

technical field [0001] The invention relates to a fly maggot protein degrading bacterium with high efficiency, which belongs to the technical field of agricultural microbes, uses microbial fermentation to degrade fly maggot protein, and is suitable for the production of high-quality protein feed. Background technique [0002] In recent years, with the rapid development of my country's feed industry, the shortage of protein feed raw materials has seriously restricted the development of my country's feed industry. At present, my country mainly relies on imported fishmeal to solve the problem of insufficient protein resources, so the development of new protein resources has become a hot topic of scientific research. Soybean is a high-quality protein raw material, but its high price will undoubtedly limit the development of the feed industry. Therefore, it is the current top priority to develop, seek and promote new and safe protein feed materials as soon as possible. Housefly...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/00A23K1/16C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 李立恒方俊符晨星谢文玉
Owner HUNAN AGRICULTURAL UNIV
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