Serum miRNA biomarker composition and application thereof

A biomarker and composition technology, applied in the field of biomarkers, can solve the problems of poor sensitivity of miRNA amplification, difficulty in detecting miRNAs, and analysis of PCR specificity, so as to achieve good sensitivity and specificity, improve reverse transcription efficiency, pain relieving effect
CN104450702AActive Publication Date: 2015-03-25XIAMEN UNIV
4 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2015-03-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a biomarker, and particularly relates to a serum miRNA biomarker composition and an application thereof. The serum miRNA biomarker composition comprises at least one miRNA and sequences of reverse transcription primers, forward primers and reverse primers of all miRNAs. The serum miRNA biomarker composition can be used for preparing oligozoospermia and azoospermia detection reagents. An oligozoospermia and azoospermia detection kit comprises the serum miRNA biomarker composition, Taq enzyme, dNTP, MgCl2 and a PCR buffer solution. The serum miRNA biomarker composition provided by the invention has the advantages of high sensitivity, low sample consumption, wide detection range and wide linear quantitative range.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to biomarkers, in particular to a serum miRNA biomarker composition and application. Background technique

[0002] miRNA, also known as microRNA, is a kind of single-stranded non-coding RNA composed of about 22 nucleotides, which mainly regulates the expression of target genes at the post-transcriptional level by direct cleavage of messenger RNA or indirect inhibition of translation. miRNAs have different expression patterns in different stages of individual development, such as cell differentiation, proliferation, apoptosis, etc., and in different tissues, indicating that they play an important regulatory role in development and differentiation. The study of miRNA expression profiles in tissues has found that miRNAs have strong cell, tissue or disease specificity, and these specifically expressed miNRAs are not only the basis for their functional studies, but also good disease markers. Studies in recent years have shown ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More