A composition for semi-quantitative rapid detection of nitrite and its application

A technology of nitrite and composition, applied in the field of chemical detection, can solve the problems of easy oxidation, inaccurate test results by test paper method, inability to obtain test paper products, etc.

Active Publication Date: 2017-08-25
贝因美(杭州)食品研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two main problems with this method: one is that standardized test paper products cannot be obtained, and the amount of water absorbed by the filter paper of the chromogenic agent during product processing and the loss in the drying process cannot be unified; the other is that the chromogenic agent is attached to the paper In contact with air in the fiber, it is easy to oxidize
These reasons have caused the inaccurate test results of the test paper method and the defects that the product is not easy to preserve.

Method used

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  • A composition for semi-quantitative rapid detection of nitrite and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: The composition of the present invention and its freeze-dried solids

[0049] Core material: 0.4% p-aminobenzene sulfonic acid solution and 0.2% naphthalene ethylene diamine hydrochloride solution mixed, volume ratio 1:1;

[0050] Wall material: mix 1 part of sodium pyrophosphate, 3 parts of phospholipid and caprylic glyceride mixture, 30 parts of whey protein, 20 parts of lactose, 1 part of 0.1% tartrate, and 50 parts of deionized water;

[0051] Composition: the core material and wall material are mixed at a volume ratio of 1:1 to obtain a composition;

[0052] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

Embodiment 2

[0053] Example 2: The composition of the present invention and its freeze-dried solids

[0054] Core material: 0.4% p-aminobenzene sulfonic acid solution and 0.2% naphthalene ethylene diamine hydrochloride solution mixed, volume ratio 1:3;

[0055] Wall material: mix 5 parts of sodium pyrophosphate, 1 part of phospholipid and caprylic glyceride mixture, 15 parts of whey protein, 50 parts of sucrose, 3 parts of 0.1% tartrate, and 200 parts of deionized water;

[0056] Composition: the core material and wall material are mixed at a volume ratio of 1:20 to obtain a composition;

[0057] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

Embodiment 3

[0058] Example 3: The composition of the present invention and its freeze-dried solids

[0059] Core material: 0.4% p-aminobenzene sulfonic acid solution and 0.2% naphthalene ethylene diamine hydrochloride solution mixed, volume ratio 1:5;

[0060] Wall material: 3 parts of sodium pyrophosphate, 5 parts of phospholipid and caprylic glyceride mixture, 20 parts of soy protein, 35 parts of lactose, 2 parts of 0.1% tartrate, and 100 parts of deionized water;

[0061] Composition: mix the core material and wall material at a volume ratio of 1:10 to obtain a composition;

[0062] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

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Abstract

The invention relates to the field of chemical detection, and specifically discloses a composition for semi-quantitative rapid detection of nitrite and an application thereof. The composition of the present invention includes a core material and a wall material, the core material includes 0.4% p-aminobenzenesulfonic acid and 0.2% naphthaleneethylenediamine hydrochloride, and the wall material includes sodium pyrophosphate, phospholipids and caprylic glyceride Blend, soy protein or whey protein, lactose or sucrose, 0.1% tartrate and deionized water. The present invention uses the wall material to embed the core material of p-aminobenzenesulfonic acid solution and naphthalene ethylenediamine hydrochloride solution, so that the wall material can not only effectively carry and protect the core material, but also quickly disintegrate when it contacts milk and dairy products, Quick response, complete detection, high detection accuracy and sensitivity.

Description

Technical field [0001] The invention relates to the field of chemical detection, in particular to a composition for semi-quantitative and rapid detection of nitrite and its application. Background technique [0002] Nitrite is an important detection index for raw materials of milk and dairy products, especially milk powder products. my country's national standard GB 2762 clearly stipulates the limits of nitrate and nitrite in milk powder. [0003] Nitrite is toxicologically classified as a highly toxic substance. When it enters the blood, it changes oxyhemoglobin into methemoglobin, thereby losing oxygen carrying capacity, leading to hypoxia in tissues and purpura, and has teratogenic and carcinogenic effects. The harm of WHO / FAO to nitrite is described as strong toxicity. The oral LD50 of mice is 200mg / kg body weight, the severe dose of adult is 0.3g-0.5g, and ADI=0.2mg / kg body weight. [0004] Normal raw milk does not contain nitrite, and nitrite in the ecosystem mainly comes fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 储小军富鑫华家才姜艳喜石红张素文
Owner 贝因美(杭州)食品研究院有限公司
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