A kind of preparation method of porcine pepsin and peptin

Abstract

The invention discloses a preparation method of porcine pepsin and gastric mucin, and the preparation method is designed for achieving the purpose of overcoming the defects that by using traditional pepsin preparation methods, pepsin is easily degenerated, organic solvents hurts the bodies of operators, and the cost is high, and the like. The method comprises the following steps: carrying out crude extraction on the mucosa of a basilar part of a porcine stomach by using a hydrochloric acid; then, by using the properties that pepsin is high in solubility and stable in enzyme activity in a weakly acidic solution, and gastric mucin is precipitated after meeting a weak acid, extracting and separating porcine pepsin and gastric mucin by using an acidic aqueous solution replacing acetone; and carrying out centrifugation, filtration, ultrafiltration, drying, and the like on the obtained product, so that porcine pepsin and gastric mucin are obtained. In case that the method is adopted for preparing porcine pepsin and gastric mucin, the activity of pepsin is protected, and meanwhile, because no organic solvent is used, the safety factor of operating is improved, and no harm is caused to the bodies of workers; and the cost is reduced, and the production capacity is increased, so that the method has broad market prospect.

Description

Technical field [0001] The invention relates to a method for preparing pepsin and gastricin, in particular to a method for preparing pepsin and gastricin from pig gastric mucosa. Background technique [0002] Pepsin is extracted from the gastric mucosa of pigs. It is a digestive protease secreted by the main cells of the gastric mucosa in the stomach. Its function is to break down the protein in food into small peptide fragments. At present, the stomach is used for medicine. Protease is generally extracted from pig gastric mucosa and used for indigestion. Gastricin is a mucin obtained by digestion and extraction of pig gastric mucosa. It has anti-pepsin decomposition and weak antacid effects. It can form a membrane in the stomach to cover the ulcer surface, reduce the stimulation of gastric acid, and be beneficial to the healing of the ulcer surface. The separation methods of pepsin include fractional salting-out separation, organic solvent separation and substrate affinity sepa...

AI Technical Summary

Problems solved by technology

The separation methods of pepsin include fractional salting-out separation, organic solvent separation and substrate affinity separation, etc. At present, the separation method of pig pepsin at home and abroad is generally to separate the crude extract prepared by the acid method through an organic solvent to obtain Mixed pepsin including pepsin A, pepsin B, pepsin C, pepsin D, mucous protein, peptin, etc. The organic solvent method generally uses water-soluble organic solvents such as acetone or ethanol, although organic solvents precipitate proteins. Well, the solvent is easy to remove, but the disadvantage is that it is easy to denature enzymes and active proteins, so the operation requires strict conditions. At the same time, the properties of the material before drying in the traditional process determine that only hot air circulation can be used, so the production capacity is also greatly limited. In addition, organic solvents are flammable and explosive, use a large amount, and cost high. Due to the certain toxicity of acetone, it will also cause certain harm to the health of production workers.