Production method of yeast extract of disodium 5'-inosinate and disodium 5'-guanylate

A technology of disodium homoinosinate and disodium guanylate, applied in food science, application, food preparation, etc., can solve the unpublished production of natural high-fresh yeast extract of homoinosinate disodium, which cannot be used Waste beer yeast slime treatment, no mention of product bitterness and peculiar smell, etc., to achieve good clarity, high total nitrogen and ammonia nitrogen, and high protein utilization

Inactive Publication Date: 2015-04-08

SHANDONG BIO SUNKEEN

7 Cites 7 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0007] However, the above-mentioned prior art documents do not disclose the high-fresh yeast extract of natural homoinosinate disodium and disodium guanylate produced from waste brewer's yeast sludge.

There is no mention of how to solve the problems of bitterness and peculiar taste, and insufficient mellowness and umami...

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Abstract

The invention provides a method for treating waste beer yeast paste to produce yeast extract of disodium 5'-inosinate and disodium 5'-guanylate (I+G). The method comprises the following steps: (1) separating or squeezing the waste beer yeast paste; (2) debittering; (3) self-dissolving; (4) carrying out enzymolysis; (5) raising the temprature and carrying out enzyme deactivation; (6) separating and evaporating; and (7) drying. According to the method provided by the invention, the content of the obtained yeast extract of the disodium 5'-inosinate and the disodium 5'-guanylate is high; additives are not added and the utilization rate of protein is high; and the content of total nitrogen and ammonia nitrogen is high. According to the method provided by the invention, the bitter taste of the product can be reduced and the umami is enhanced; and meanwhile, the mellow taste is improved and the peculiar smell is balanced, and the comprehensive taste of the product can be effectively improved.

Application Domain

Food preparation

Technology Topic

ChemistryEnzyme +9

Examples

Experimental program(5)

Example Embodiment

[0045] Example 1: High I+G yeast extract product 1 obtained according to the method of the present invention

[0046] Main equipment: autolysis tank, evaporator; separator, spray tower.

[0047] Production is carried out according to the following process (based on 30 kg of beer yeast paste):

[0048] Preferably, fresh beer yeast paste is transported and stored in a refrigerated truck at a temperature not higher than 15°C, and the storage process is preferably not higher than 5°C. Pass through a 60-200 mesh screen to remove hops and other foreign matter.

[0049] Blend the processed yeast paste to 8-18%, and add 2.0% of the total dry weight of the yeast paste with NaHCO 3 After treating for 2 hours below 10°C, separate it again. Add 1.5% NaHCO 3 It is treated at 10°C or less for 1.5 hours, separated and washed to pH 6.0.

[0050] The concentration of the yeast paste after the above treatment is adjusted to a solid content of 10%, and 7% alcohol is added as an autolysis accelerator. The temperature is raised to 51°C, the pH is natural, and the protease is added 0.5‰, enzymatically hydrolyzed for 4 hours, and then heated to 60°C , Adjust the pH to 5.5, add 1‰ Enzyme RP-1G for enzymatic hydrolysis for 15 hours, then add 1‰ Deamizyme 50000G for enzymatic hydrolysis for 10 hours without changing the temperature and the pH. The autolyzed enzyme hydrolyzed yeast mud was heated to 80°C and kept for 20 minutes to inactivate the enzyme.

[0051] Separate the enzyme-inactivated yeast mud, take samples during the separation process to observe the clarity of the separated clear phase to ensure that the separation process is normal. Then the supernatant is evaporated by three-effect or four-effect evaporation until the dry matter is about 40%.

[0052] The evaporated extract is directly fed into the spray tower to spray powder.

[0053] In the present invention, the protease, Enzyme RP-1G enzyme, and Deamizyme 50000G are all commercially available, for example, from Amano Enzyme Products Co., Ltd., the total number of colonies ≤10000 cfu/g, Escherichia coli, Bacillus cereus and other pathogenic bacteria Not detectable; the sodium bicarbonate, sodium hydroxide, phosphoric acid, alcohol, etc. used are all food-grade or pharmaceutical-grade products, which can be purchased from the market.

Example Embodiment

[0054] Example 2: Yeast extract product 2 obtained according to the method of the present invention

[0055] The main equipment and materials are the same as above.

[0056] Production is carried out according to the following process (based on 30 kg of beer yeast paste):

[0057] Preferably, fresh beer yeast paste is transported and stored in a refrigerated truck at a temperature not higher than 15°C, and the storage process is preferably not higher than 5°C. Pass through a 60-200 mesh screen to remove hops and other foreign matter.

[0058] Blend the processed yeast paste to 8-18%, and add 1.5% of the total dry weight of the yeast paste with NaHCO 3 After treating for 2 hours below 10°C, separate it again. Add 1.0% NaHCO 3 It is further processed for 1.0 hour below 10°C, separated and washed to pH 6.0.

[0059] The concentration of the yeast paste after the above treatment is adjusted to a solid content of 12%, 9% alcohol is added as an autolysis accelerator, the temperature is raised to 48°C, the pH is natural, the protease is 0.5‰, the enzyme hydrolysis is 3 hours, and then the temperature is raised to 50°C , Adjust the pH to 6.0, add 3‰ Enzyme RP-1G for enzymatic hydrolysis for 13 hours, then add 3‰ Deamizyme 50000G for enzymatic hydrolysis for 9 hours without changing the temperature and the pH. The autolyzed yeast slurry is heated to 85°C and kept for 20 minutes to inactivate the enzyme.

[0060] Separate the enzyme-inactivated yeast mud, take samples during the separation process to observe the clarity of the separated clear phase to ensure that the separation process is normal. Then the supernatant is evaporated by three-effect or four-effect evaporation until the dry matter is about 45%.

[0061] The evaporated extract is directly fed into the spray tower to spray powder.

[0062] In the present invention, the raw materials used are as described above, and all are available on the market.

Example Embodiment

[0063] Example 3: Yeast extract product 3 obtained according to the method of the present invention

[0064] The main equipment and materials are the same as above.

[0065] Production is carried out according to the following process (based on 30 kg of beer yeast paste):

[0066] Preferably, fresh beer yeast paste is transported and stored in a refrigerated truck at a temperature not higher than 15°C, and the storage process is preferably not higher than 5°C. Pass through a 60-200 mesh screen to remove hops and other foreign matter.

[0067] Blend the processed yeast paste to 8-18%, and add 2.0% of the total dry weight of the yeast paste with NaHCO 3 After treating for 1 hour at 10°C or less, separate again. Add 1.5% NaHCO 3 It is treated at 10°C or less for 2.0 hours, and then separated and washed to pH 6.0.

[0068] The concentration of the yeast paste after the above treatment is adjusted to 15% solid content, 9% alcohol is added as an autolysis accelerator, the temperature is raised to 45℃, the pH is natural, the protease is 0.5‰, the enzyme hydrolysis is 6 hours, and then the temperature is raised to 65℃ , Adjust the pH to 5.5, add 5‰ Enzyme RP-1G for enzymatic hydrolysis for 10 hours, then add 5‰ Deamizyme 50000G for enzymatic hydrolysis for 8 hours without changing the temperature and the pH. The autolyzed yeast slurry is heated to 75°C and kept for 20 minutes to inactivate the enzyme.

[0069] Separate the enzyme-inactivated yeast mud, take samples during the separation process to observe the clarity of the separated clear phase to ensure that the separation process is normal. Then the supernatant is evaporated by three-effect or four-effect evaporation until the dry matter is about 45%.

[0070] The evaporated extract is directly fed into the spray tower to spray powder.

[0071] In the present invention, the raw materials used are as described above, and all are available on the market.

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Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

Reduce bitterness
Improve protein utilization

Novel processing method for green tea

InactiveCN103349097AReduce bitternessImprove freshnessPre-extraction tea treatmentCaffeineChemistry

Owner:JIANGSU MINGYUAN TEA TECH SCI

Processing technology of green tea with flower fragrance

InactiveCN102742677AReduce bitternessGuaranteed greennessPre-extraction tea treatmentChemistryQuality characteristics

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Identification of human T2R receptors that respond to bitter compounds that elicit the bitter taste in compositions, and the use thereof in assays to identify compounds that inhibit (block) bitter taste in composition and use thereof

ActiveUS20090274632A1Reduce bitternessSuppress bitternessOrganic active ingredientsCosmetic preparationsDrugT2R receptors

Owner:SENOMYX INC

Method for brewing emblic leafflower fruit wine

InactiveCN101812391AReduce bitternessFull of nutritionAlcoholic beverage preparationNutrientPhyllanthus emblica

Owner:PANZHIHUA UNIV

Soluble Coffe Product

InactiveUS20080166451A1Reduce bitternessReduce levelGreen coffee treatmentUnwanted substance removalSolventEnzyme

Owner:NESTEC SA

Production method of yeast extract of disodium 5'-inosinate and disodium 5'-guanylate

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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