Corn tissue specificity promoter and applications thereof

Abstract

The invention discloses a corn tissue specificity promoter and applications thereof, and belongs to the field of separation and applications of plant tissue specificity promoters. The invention firstly discloses the tissue specificity promoter as shown by nucleotide sequence SEQ ID NO.1 separated from corn, and the tissue specificity promoter sequence is further truncated to obtain a truncated promoter segment as shown by SEQ ID NO.2 or SEQ ID NO.3 still with the promoter function. The invention further discloses the applications of the tissue specificity promoter or the truncated promoter segment in aspects of enhancing crop seed quality, improving crop characters and cultivating new varieties of transgenic plants and the like.

Description

technical field [0001] The present invention relates to a promoter isolated from plants, in particular to a tissue-specific promoter isolated from corn (Zea mays), and the present invention further relates to a recombinant plant expression vector containing the tissue-specific promoter and a recombinant host cell , the present invention further relates to their application in improving crop seed quality, improving crop traits, cultivating new plant varieties, etc., and belongs to the field of separation and application of plant tissue-specific promoters. Background technique [0002] The promoter is a DNA sequence that RNA polymerase specifically recognizes and binds to, and is an important cis-acting element, generally located in the upstream region of the 5' end of the structural gene. Gene regulation in higher plants is mainly carried out at the transcription level. The promoter controls the initiation time and expression level of gene expression, and also plays a decisiv...

AI Technical Summary

Problems solved by technology

At present, most of the promoters used in plant genetic engineering are constitutive promoters, which enable the high-level expression of exogenous target genes in various plant tissues and parts, but constitutive promoters also have many disadvantages, for example, they cannot be efficiently expressed in time and space. Regulating the expression of target genes, excessive consumption of intracellular material and energy (Gittins JR, Pellny TK, Hiles ER, Rosa C, Biricolti S, et al. (2000) Transgene expression driven by heterologous ribulose-1,5-bisphosphate carboxylase / Oxygenase small-subunit gene promoters in the vegetable tissues of apple(Malus pumila mill.).Planta 210:232-240.); A large number of heterologous proteins or metabolites accumulate in plants, which breaks the metabolic balance of plants and is not conducive to plant growth (Robinson DJ (1996) Environmental risk assessment of releases of transgenic plants containing virus-derived inserts. Transgenic research 5:359-362.); easy to cause gene silencing or co-suppression (Kumpatla SP, Chandrasekharan MB, Iyer LM, Guofu L ,Hall TC(1998)Genome intruder scanning and modulation systems and transgene silencing.Trends in Plant Science 3:97-104;Mette MF,Aufsatz W,van der Winden J,Matzke MA,Matzke AJ(2000)Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J 19:5194-5201.)

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