Method for comprehensively extracting protein, polysaccharide and polyphenol from avocado kernels
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A comprehensive extraction and avocado technology, applied in the field of comprehensive utilization and development of avocado resources, to achieve mild production conditions, simplify production procedures, improve purity and production efficiency
Inactive Publication Date: 2015-04-22
GUANGXI POLYTECHNIC
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[0006] At present, there have been related reports on the extraction of avocado kernel protein, but avocado kernels are also rich in active ingredients such as polysaccharides and polyphenols in addition to protein. At present, there is no relatively mature process that can comprehensively extract protein from avocado kernels , polysaccharides, polyphenols and other active substances, but cannot reasonably comprehensively utilize and develop avocado resources
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Embodiment 1
[0030] (1) 50g of avocado kernel powder is leached twice with petroleum ether (30-60℃) at 50°C, the ratio of solid to liquid is 1g:10mL, each time for 2.0h, filtered, combined extracts, concentrated and recovered petroleum ether to obtain oil Pear kernel oil and defatted pear kernel powder.
[0031] (2) Add pure water to the defatted avocado kernel powder, the ratio of solid to liquid is 1g: 15mL, extract at 60°C for 2.5h, filter, filter, and dry the residue to obtain dietary fiber, and the extract is concentrated to the original volume Half of it was adjusted to pH=4.0 with 5% hydrochloric acid solution, centrifuged to obtain the precipitated protein, and dried to obtain the protein.
[0032] (3) The aqueous phase was taken, adsorbed with D3520 macroporous resin for 3 hours, eluted with 1 times column volume of pure water, collected the effluent, and spray-dried to obtain polysaccharides.
[0033] (4) The D3520 macroporous resin was eluted with 20% ethanol solution to remove...
Embodiment 2
[0035] (1) 500g of avocado kernel powder was leached twice with petroleum ether (60-90℃) at 70°C, the ratio of solid to liquid was 1g:5mL, each time for 2.5h, filtered, combined extracts, concentrated and recovered petroleum ether to obtain oil Pear kernel oil and defatted pear kernel powder.
[0036] (2) Add pure water to the defatted avocado kernel powder, the ratio of solid to liquid is 1g:10mL, extract at 70°C for 3.0h, filter, filter, and dry the residue to obtain dietary fiber, and the extract is concentrated to the original volume 1 / 2, adjust the pH=4.6 with 2% sulfuric acid solution, centrifuge to obtain the precipitated protein, and dry to obtain the protein.
[0037] (3) The aqueous phase was taken, adsorbed with D3520 macroporous resin for 5 hours, eluted with 1.5 times column volume of pure water, collected the effluent, and spray-dried to obtain the polysaccharide.
[0038] (4) D3520 macroporous resin was eluted with 15% ethanol solution to remove impurities, and...
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Abstract
The invention provides a method for comprehensively extracting protein, polysaccharide and polyphenol from avocado kernels. The method provided by the invention comprises the following steps: (1) grinding, drying and degreasing avocado kernels, then performing extraction by using ethanol/water, concentrating, adjusting a pH value of an extracting solution to reach a protein isoelectric point so as to ensure that protein is precipitated, performing solid-liquid separation, and drying to obtain protein; (2) performing macroporous resin adsorption on supernatant liquor obtained by protein precipitation, and drying an outflow fluid to obtain polysaccharide with relatively high purity; and (3) eluting macroporous resin by using an ethanol solution, and concentrating and drying an eluant to obtain polyphenol with relatively high purity. According to the method provided by the invention, three products namely protein, polysaccharide and polyphenol are sequentially obtained from the avocado kernels; and moreover, the method is simple in process, mild in production condition and relatively high in purity of the obtained products, and is suitable for industrial production; and solvents used by the method are mainly water and ethanol, so that the method is small in environmental pollution and low in production cost.
Description
technical field [0001] The invention relates to a method for comprehensively extracting proteins, polysaccharides and polyphenols from avocado kernels, and belongs to the technical field of comprehensive utilization and development of avocado resources. Background technique [0002] Avocado, also known as avocado, avocado or avocado, is an evergreen tree species of the genus Avocado in the Lauraceae family. Its pulp is rich in fatty acids and does not contain cholesterol. It has high nutritional value and enjoys the reputation of "forest butter" and "source of life". [0003] Introduced to Fujian, Guangdong and other provinces in the 1920s, after years of introduction, it has been distributed in Hainan, Fujian, Guangdong, Guangxi, Yunnan, Sichuan and Zhejiang provinces since the 1950s, with relatively abundant yields. At present, the research on avocado at home and abroad mainly focuses on the cultivation and preservation of avocado, and the extraction and utilization of av...
Claims
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