Aspirin-based carbon quantum dot and biological application thereof

A carbon quantum dot and aspirin technology, which is applied in the field of nano-drugs, can solve the problems of biological activity retention, unseen research, etc., and achieves the effects of low risk, simple experimental operation, and good experimental repeatability.

Inactive Publication Date: 2015-04-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, carbon quantum dots are mainly used in biomedicine for bioimaging or drug / gene transfer, but the research on making drugs directly into carbon quantum dots has not been reported. Whether the biological activity of drugs is preserved after making carbon quantum dots no more research

Method used

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  • Aspirin-based carbon quantum dot and biological application thereof
  • Aspirin-based carbon quantum dot and biological application thereof
  • Aspirin-based carbon quantum dot and biological application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Preparation of aspirin-based carbon quantum dots: 2 g of acetylsalicylic acid was added to 1.08 mL of an aqueous solution of hydrazine hydrate with a mass concentration of 98%, and the total volume of the entire system was 10 mL. The microwave power is 100W, and the time is 8 minutes. Aspirin-based carbon quantum dots with blue fluorescence were obtained.

[0022] (2) Separation and purification of carbon quantum dots: add 80 mL of deionized water to the carbon quantum dots prepared in step (1), ultrasonically disperse the product, separate and purify the prepared carbon quantum dots with a molecular weight of 3500, and dialysis for 7 Days later, the obtained product was filtered with a water-based filter head with a particle size of 0.22 μm, concentrated to 10 mL after rotary evaporation, and then freeze-dried to obtain aspirin-based carbon quantum dot powder (brown yellow), with a yield of about 50 mg.

[0023] Observation under a transmission electron microscope...

Embodiment 2

[0025] (1) Preparation of aspirin-based carbon quantum dots: 1 g of acetylsalicylic acid was added to an aqueous solution containing 1.08 mL of 98% hydrazine hydrate, and the total volume of the entire system was 10 mL. The microwave power is 100W, and the time is 7 minutes. Aspirin-based carbon quantum dots with blue fluorescence were obtained.

[0026] (2) Separation and purification of carbon quantum dots: add 50 mL of deionized water to the carbon quantum dots prepared in step (1), ultrasonically disperse the product, separate and purify the prepared carbon quantum dots with a molecular weight of 3500, and dialysis for 7 Days later, the obtained product was filtered with a water-based filter head with a particle size of 0.22 μm, concentrated to 5 mL after rotary evaporation, and freeze-dried to obtain aspirin-based carbon quantum dot powder (brown yellow), with a yield of about 30 mg. The resulting carbon quantum dots have strong fluorescent properties, such as figure 2...

Embodiment 3

[0028] (1) Preparation of aspirin-based carbon quantum dots: 2 g of acetylsalicylic acid was added to an aqueous solution containing 0.54 mL of 98% hydrazine hydrate, and the total volume of the entire system was 10 mL. The microwave power is 100W, and the time is 8 minutes. Aspirin-based carbon quantum dots with blue fluorescence were obtained.

[0029] (2) Separation and purification of carbon quantum dots: add 60 mL of deionized water to the carbon quantum dots prepared in step (1), ultrasonically disperse the product, separate and purify the prepared carbon quantum dots with a molecular weight of 3500, and dialysis for 7 Days later, the obtained product was filtered with a water-based filter head with a particle size of 0.45 μm, concentrated to 5 mL after rotary evaporation, and then freeze-dried to obtain aspirin-based carbon quantum dot powder (brown yellow), with a yield of about 40 mg. After infrared testing, it can be seen that carbon quantum dots retain the acetyl g...

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Abstract

The invention discloses an aspirin-based carbon quantum dot and application thereof in aspects of cell imaging and anti-inflammatory treatment, belonging to the technical field of nano-drugs. The invention in particular relates to a carbon quantum dot with strong fluorescent properties prepared from a hydrazine hydrate solution by utilizing a microwave heating method under the condition that aspirin dissolution is promoted. The carbon quantum dot prepared by the method is very stable under hyper-salt concentration and physiological pH conditions, blue-fluorescence is maintained, the carbon quantum dot can enter the cell nucleus, and the cells are effectively traced; and meanwhile, the original anti-inflammatory effect of aspirin is remained, and inflammatory cells can be effectively inhibited in an acute inflammation model constructed by utilizing carrageenin. In addition, the prepared aspirin carbon quantum dot is low in toxicity and does not influence liver and kidney functions and organs such as the heart, liver, spleen and kidney in in-vivo application, and the aim that the nanometer material has dual effects of diagnosis and treatment can be achieved.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and specifically relates to the preparation of an aspirin-based carbon quantum dot, which can be obtained by heating aspirin and hydrazine hydrate with microwaves, has strong fluorescent properties, and retains the drug activity of aspirin, so it can be Applied to cell imaging and anti-inflammatory therapy. Background technique [0002] In recent years, nanotechnology has been used in the transfer and treatment of genes / drugs, or as a diagnostic probe in medicine. The combination of nanotechnology and medicine is called nanomedicine. The core problem facing this field is how to design a multifunctional nanomaterial that can be used not only for disease diagnosis but also for disease treatment. As a new member of the family of carbon nanomaterials, carbon quantum dots with fluorescent properties have attracted widespread attention from scholars in the field of nanotechnology. These surface-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02C07C69/157C09K11/06A61K49/00A61K31/616A61P29/00
Inventor 孙宏晨徐晓薇张恺王丹丹林崇涛杨柏
Owner JILIN UNIV
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