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A kind of detection method of creatinine

A detection method, creatinine technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of expensive instruments, complicated operation, high cost, etc., achieve high sensitivity, fast and convenient detection, and ensure high reproducible effect

Active Publication Date: 2017-05-17
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these methods have the disadvantages of time-consuming, expensive equipment, complicated operation, and high cost, and some methods are only limited to detection and analysis in laboratories.

Method used

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  • A kind of detection method of creatinine
  • A kind of detection method of creatinine
  • A kind of detection method of creatinine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Flow injection-chemiluminescence analysis platform, see figure 1 , figure 1 It is a schematic diagram of the structure of the flow injection-chemiluminescence analysis platform: including the sample circulation pipelines A, B, and C, hydrogen peroxide solution and buffer solution are added at A, cobalt ion solution is added at B, and water is added at C; D is the flow Syringe pump, E is the injection valve; F is the chemiluminescence detector; G is the waste liquid pool; H is the computer control system.

[0041] 0.03mM cobalt ion aqueous solution, 0.1M pH9.5 sodium carbonate / sodium bicarbonate buffer solution and 0.01mM creatinine solution were mixed in the flow injection analysis device, and the concentrations of 0.1mM, 0.5mM, 1mM, 2.5mM, 5mM hydrogen peroxide solution was reacted, and the luminescence intensity of the reaction product was detected. Chemiluminescence intensity-hydrogen peroxide solution concentration curve such as figure 2 shown. Depend on figu...

Embodiment 2

[0048] Prepare a real sample:

[0049] The urine was diluted 1000 times, and creatinine was added to the urine to prepare a sample with a creatinine concentration of 10 μmol / L.

[0050] use figure 1 The flow injection-chemiluminescence assay platform shown was used for detection. Inject 1mM hydrogen peroxide / pH 9.5 0.1M sodium bicarbonate-sodium carbonate buffer solution and 0.02mM cobalt ion aqueous solution into A and B respectively, inject secondary water into C, and then add 200uL creatinine of different concentrations through injection valve E For the sample, the luminous intensity signal is recorded at F, and the concentration of the actual sample is obtained according to the linear equation through the obtained luminous intensity. See Table 1 for the results.

Embodiment 3

[0052] Prepare a real sample:

[0053] The urine was diluted 1000 times, and creatinine was added to the urine to prepare a sample with a creatinine concentration of 20 μmol / L.

[0054] use figure 1 The flow injection-chemiluminescence assay platform shown was used for detection. Inject 1mM hydrogen peroxide / pH 9.5 0.1M sodium bicarbonate-sodium carbonate buffer solution and 0.02mM cobalt ion aqueous solution into A and B respectively, inject secondary water into C, and then add 200uL creatinine of different concentrations through injection valve E For the sample, the luminous intensity signal is recorded at F, and the concentration of the actual sample is obtained according to the linear equation through the obtained luminous intensity. See Table 1 for the results.

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Abstract

The invention provides a creatinine detection method. The method comprises the following steps: mixing and reacting a cobalt ion solution, a hydrogen peroxide solution, a sodium carbonate / sodium bicarbonate buffer solution and a to-be-detected creatinine solution in a flow injection analysis device, and detecting to obtain the luminous intensity of the reaction product; and obtaining the creatinine content of the to-be-detected creatinine solution according to a preset standard curve. The hydrogen peroxide is catalyzed to oxidize the creatinine by utilizing the cobalt ions so as to give out light. Because the luminous material in the method refers to a product of the creatinine reaction, and a background signal generated by a reaction between the cobalt ions and hydrogen peroxide is low in the absence of creatinine, the detection method disclosed by the invention is high in sensitivity. Moreover, the flow injection analysis device is utilized, the high reproducibility of the mixing process and the reaction time is guaranteed, and online sample treatment and measurement can be efficiently finished in an imbalanced state, so that the detection is rapid and convenient.

Description

technical field [0001] The invention relates to the field of chemiluminescence detection, in particular to a creatinine detection method. Background technique [0002] Creatinine is the final product of creatine metabolism, and every 20 grams of muscle blood creatine can produce 1 mg of creatinine. Creatinine is mainly excreted by glomerular filtration. Clinically, creatinine detection is one of the main methods commonly used to understand renal function. It is indispensable for evaluating glomerular rate, diabetic nephropathy, renal failure and nephrotic syndrome. index of. Moreover, creatinine can also be used as a biomarker in the clinical diagnosis of acute myocardial infarction, chronic heart failure, colorectal cancer and muscular dystrophy. Therefore, it is meaningful to establish a new rapid, efficient and low-cost creatinine analysis method. [0003] Conventional creatinine detection methods include Jaffé method and enzyme-catalyzed method. The routinely used Ja...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 徐国宝塞玛·韩霓芙启黎明皮特·约翰
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES