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Chickpea sprout extract and application thereof

A technology of chickpea sprouts and extracts, applied in the directions of drug combinations, plant raw materials, cosmetic preparations, etc., can solve problems such as permanent depigmentation, skin side effects, contact dermatitis, etc.

Inactive Publication Date: 2015-05-06
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional skin whitening and anti-aging agents often use chemical substances. Although these chemical substances can quickly achieve whitening and anti-aging effects, they have toxic side effects on the skin. Long-term use can cause contact dermatitis or permanent depigmentation and other adverse effects

Method used

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  • Chickpea sprout extract and application thereof
  • Chickpea sprout extract and application thereof
  • Chickpea sprout extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the cultivation of chickpea sprouts

[0051] Take chickpeas (identified as Dixi type chickpeas by Professor Wang Qi of Shihezi University), wash them, soak them in warm water for 6-8 hours, take them out and put them in the bean sprouts box. The temperature of the bean sprouts box is 30℃, and the watering time 5s, and the watering interval is 180min. When the bean sprouts grow to about 5-6cm, separate the bean sprouts from the watercress, take the bean sprouts and set aside.

Embodiment 2

[0052] Example 2 Preparation of Chickpea Sprout Extract

[0053] Take 150 grams of fresh chickpea sprouts and add 1500 ml of 70% ethanol to ultrasonically extract twice, each time for 1 hour, filter, combine the filtrates, and obtain bean sprout residues for later use, concentrate the extract until it has no alcohol taste, and obtain the crude extract of chickpea sprouts. Dissolved to about 100ml, enriched by AB-8 macroporous resin, eluted with 30% and 70% ethanol, 3-4 times the column volume, collected the eluate, and distilled off the solvent under reduced pressure to obtain 30% chickpea sprouts site and 70% site.

Embodiment 3

[0054] Embodiment 3 antioxidant effect

[0055] Use DPPH free radical scavenging effect to measure the antioxidant effect of the sample. Take the sample solution and DPPH solution and put them into the same stoppered test tube according to the equal volume ratio, shake well, react for 30 minutes under dark conditions, and measure the absorbance at 525nm with a spectrophotometer. T. Simultaneously measure the absorbance C of the DPPH solution and the equal volume of pure water mixture, and the absorbance T of the test solution and the equal volume of 95% ethanol mixture 0 . Calculate the clearance rate according to the following formula: clearance rate=[1-(T-T 0 ) / C]×100%. At the same time, this experiment uses Vc as a control.

[0056] Half clearance rate (IC 50 ) Calculation: The half-clearance rate refers to the concentration of the required sample when the clearance rate is 50%. Prepare the antioxidants to be tested into a series of solutions, measure the scavenging r...

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Abstract

The invention provides a chickpea sprout extract and an application thereof. The chickpea sprout extract is prepared by using a method including the following steps: A, taking chickpeas, completely cleaning, soaking the chickpeas in warm water until the surfaces of the chickpeas are wrinkled, then taking out and placing the wrinkled chickpeas in a bean sprout box, spraying water once at short intervals until bean sprouts grow to reach about 5cm, and separating the bean sprouts and bean halves to obtain the bean sprouts; and B, adding ethanol into the bean sprouts to perform ultrasonic extraction, filtering, combining filtrates to obtain bean sprout residues for later use, concentrating an extracting solution until no alcohol taste is produced, performing AB-8 macroporous resin enrichment, sequentially performing ethanol elution and eluent collection, and removing solvents by virtue of reduced pressure distillation to obtain the chickpea sprout extract. The chickpea sprout extract provided by the invention has very good effects of skin whitening, resisting aging and inhibiting tyrosinase activity, can be applied to the fields of cosmetics, health foods and medicines, and can be particularly applied to the cosmetics.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, in particular to a chickpea sprout extract and its application. Background technique [0002] Whitening and anti-aging cosmetics have always been the pursuit and yearning of women, and they are also eternal topics in the beauty industry. In recent years, with the development of social economy and the progress of science and technology, people's demand for cosmetics has also increased. [0003] The skin is the most exposed organ in the aging process. Skin collagen accounts for about 75% of the weight of dry skin. It is an important component of the extracellular matrix of the skin, functions as a support for the skin structure, and provides skin resistance and elasticity, etc. important function. The production and decomposition of collagen in normal skin are in a balanced state under the action of biological regulation. With the increase of age and environmental factors such as ultravio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K36/48A61Q19/02A61Q19/08A61P17/00A61P17/18
Inventor 金家宏李慧陈明华
Owner JALA GROUP CORPORATION