Oligonucleotide aptamer for specifically identifying patulin

A technology of patulin and oligonucleotides, which is applied in the preparation of test samples, DNA/RNA fragments, material inspection products, etc., can solve the problems of high detection cost, complicated operation, low detection limit, etc., and achieve Stable chemical properties, good reproducibility, and convenient results

Active Publication Date: 2015-05-06
JIANGNAN UNIV
View PDF2 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have low detection limits and high precision, but the operation is complicated and the detection cost is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oligonucleotide aptamer for specifically identifying patulin
  • Oligonucleotide aptamer for specifically identifying patulin
  • Oligonucleotide aptamer for specifically identifying patulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The following is a further description of screening nucleic acid aptamers that specifically bind to patulin by SELEX technology.

[0013] 1. Synthesize random single-stranded DNA library and primers (synthesized by IDT)

[0014] Random single-stranded DNA (ssDNA) library: 5′-CAGCTCAGAAGCTTGATCCT(N40)GACTCGAAGTCGTGCATCTG-3′, a random ssDNA library with a length of 80 nt was constructed, with fixed primer sequences at both ends and a random sequence of 40 bases in the middle, the library capacity for 10 14 Above; upstream primer Ⅰ: 5′-CAGCTCAGAAGCTTGATCCT-3′; downstream primer Ⅱ: 5′-CAGATGCACGACTTCGAGTC-3′, the random ssDNA library and the two primers were prepared into 100 μmol / L stock solution with TE buffer and stored at -20°C spare.

[0015] 2. Preparation and purification of ssDNA

[0016] The reaction system is: template DNA 5 μL, FAM-labeled upstream primer 1 μL (20 mM), phosphorylated downstream primer 0.5 μL (20 mM), dNTPmix (25 mM) 0.5 μL, 10×PCR amplificatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an oligonucleotide aptamer for specifically identifying patulin. Through a graphene oxide-based systematic evolution technology of ligands by exponential enrichment, a single stranded oligonucleotide aptamer capable of specifically identifying patulin is acquired. The aptamer can be converted into a report aptamer by marking functional groups and the like. The aptamer is used for detecting patulin. The sequence of the aptamer has a wide application prospect in accurately and quickly detecting patulin in a food.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to the preparation of a nucleic acid aptamer with high specificity and high affinity to patulin by using a SELEX technology (exponentially enriched ligand system evolution technology). The ligand provides the basis for the rapid detection of patulin. Background technique [0002] Patulin is a fungal metabolite produced by the genera Penicillium, Aspergillus, and Clothes, and is mainly found in apples and their products. Patulin is stable to heat, its structure cannot be destroyed by heating at 100°C for 15 minutes, and it can be preserved for a long time in acidic solution and organic solvent. Patulin has been used as an antibiotic because it inhibits the growth of a variety of bacteria. It has acute toxicity such as convulsions, intestinal bleeding, pulmonary hemorrhage, and kidney damage. It also has neurotoxicity, immunotoxicity, genotoxicity, and teratogenicity. , carcinog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N33/53G01N1/40
Inventor 王周平张维潇段诺吴世嘉夏雨
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap