30results about How to "Wide range of targets" patented technology

The invention discloses an oligonucleotide aptamer for specifically identifying patulin. Through a graphene oxide-based systematic evolution technology of ligands by exponential enrichment, a single stranded oligonucleotide aptamer capable of specifically identifying patulin is acquired. The aptamer can be converted into a report aptamer by marking functional groups and the like. The aptamer is used for detecting patulin. The sequence of the aptamer has a wide application prospect in accurately and quickly detecting patulin in a food.

The invention relates to a radio detector for detecting the stepping force of bikes, which belongs to a detector for detecting the stepping force of bikes. The detector comprises pedals and circuits; wherein, the pedals can be fixed and connected with a beam by screws. A pressure sensor is fixed and connected with the beam which is fixed and connected with a bearing sleeve. The bearing sleeve is fixed and connected with a stepping axle of a bike. Circuit boards are embedded in the pedals. The circuit part includes that an independent power supply, the pressure sensor, an amplifying circuit, an A / D converter circuit, a radio transmitting and receiving module and a single chip computer are connected with a liquid crystal displays and a memory which is connected with a dual-port RAM. The invention has the advantages that sensors are arranged on the pedals and directly detect the force; circuit boards and sensors are designed to be integrated; and the range of using objects is very wide.

The invention discloses a metabolite-peptide-aptamer rapid screening method based on the molecular docking simulating computation technology. The metabolite-peptide-aptamer rapid screening method includes the steps that (1) a target metabolite is determined; (2) a potential polypeptide library is obtained, wherein binding force exists between the potential polypeptide library and the target metabolite; (3) the potential polypeptide library and the target metabolite are subjected to molecular docking forecasting. The metabolite-peptide-aptamer rapid screening method is high in speed and wide intarget range, a large quantity of manpower and material resources are not required, harm to experimenter bodies is avoided, and limiting factors of wide application of current peptide aptamer are avoided. According to the metabolite-peptide-aptamer rapid screening method based on the molecular docking simulating computation technology, the peptide-aptamer screening procedure can be simplified, the library capacity is increased to a greatest degree, the metabolite peptide aptamer with the suitable specificity and affinity is thus conveniently screened out, and the metabolite-peptide-aptamer rapid screening method is used for designing such as kit and sensor detection, and is applied to detection of the field such as nutriology, biology and clinical diagnosis.

The invention discloses a preparation method for a CrN coating on a surface of a nuclear zirconium alloy and a product. The sputtering power, the matrix temperature and the bias voltage are adjusted by the multi-target magnetron sputtering technology; and the CrN coating is prepared on the surface of the Zr alloy matrix. According to the invention, the energy of deposited particles is increased byadjusting the bias voltage of the matrix, so the bombardment effect of the deposited particles on the surface of the Zr alloy matrix is enhanced, the bonding at the interface between the CrN coatingand the Zr alloy matrix is promoted, and then the bonding force of the CrN coating and the Zr alloy matrix is increased; the CrN coating with uniform compactness, excellent bonding performance and high thickness and deposited on the surface of the Zr alloy matrix is obtained; the CrN coating is of important scientific significance and engineering application value to a protective coating preparedon the surface of the zirconium alloy and meeting the engineering application thickness (above 10 microns).

The invention relates to a cadmium ion aptamer and a related screen-printed electrode electrochemical biosensor. The cadmium ion aptamer has a length of 22-28 bases, and contains the nucleic acid sequence GTGATGACCTGC. The invention also provides a screen-printed electrode electrochemical biosensor containing the cadmium ion aptamer. Based on the specific recognition function of aptamer and cadmium ion, the invention employs DPV as the electrochemical detection technology, establishes a method for detecting cadmium ion with an electrochemical aptamer biosensor, and provides a new method and way for cadmium ion detection. The adapter and the electrochemical biosensor provided by the invention have the advantages of high specificity, high sensitivity, convenient operation, low detection cost, applicability to field detection and the like.

The invention discloses a nucleic acid aptamer usable for detecting early ovarian cancer tissues and an application of the nucleic acid aptamer in early clinical detection of ovarian cancer. A sequence of the nucleic acid aptamer is selected from any one of SEQ ID NO.1 to SEQ ID NO.8, or the sequence is optimized or modified. Compared with a traditional antibody, the nucleic acid aptamer has the advantages that the target recognition range is wide, the specificity is high, the affinity is high, the toxicity and immunogenicity are low, the in-vitro synthesis cost is low, the time is saved, theaptamer can be used for drug loading or modification, the functionality of the aptamer is enhanced, and the expected treatment target is achieved. By utilizing the short-chain nucleic acid aptamer andperforming modifying and marking, ovarian cancer epithelial cells and tissues can be identified and detected, the process is rapid, simple and convenient, and a new thought is provided for early detection and clinical treatment of the ovarian cancer.

The invention provides a biotin-avidin-lentiviral expression vector and a CAR-T (chemric antigen receptor T) cell preparation method, and aims at solving the defects of single targeting ability, complex preparation, time-consuming and labor-consuming properties and poor economical efficiency of traditional CAR-T. The method comprises the following steps of constructing an avidin-CAR lentivirus package carrier; performing virus packaging; and performing T cell separation, activation, co-stimulation structural domain gene transduction and amplification, thereby preparing CAR-T cells. On the basis of a general CAR-T treatment system, a molecular imaging detection means is introduced, a cell-based visualization treatment scheme is provided, and a CAR-T cell treatment process can be dynamically monitored in vivo in real time, so that a situation that the current CAR-T cell treatment process is lack of effective monitoring is solved. The biotin-avidin-lentiviral expression vector has the advantages of simplicity in preparation, diversity in selection, extensive targets and visualization on treatment process, and suitability for treatment of tumor patients.

The invention discloses a preparation method and application of an aminated graphene quantum dot. The preparation method includes: blending a uniform dispersion liquid of prepared graphene, ammonia water and deionized water, then transferring the mixture into an autoclave for heating, filtering out insoluble fragments by a porous inorganic membrane, then conducting heating to remove redundant amine, and then using a centrifugal filtration device of molecular weight interception membrane for ultrafiltration of the supernatant, and subjecting the obtained yellow suspension liquid to further dialysis by a Spectra / Por CE dialysis tube membrane, thus obtaining aminated graphene quantum dot. The aminated graphene quantum dot prepared by the method provided by the invention can be applied to gene delivery vectors, has no inhibiting effect on RNAnase, has protective effect on to-be-delivered miRNA, and has good selectivity and universality.

The invention discloses wheat grain fishing bait. The wheat grain fishing bait is composed of wheat grains, medicinal liquor and a soak solution. The wheat grains are mixed with the medicinal liquor and soaked for 5-30 days under the environment that the room temperature ranges from 15 DEG C to 30 DEG C; the well soaked wheat grains are taken out and dried; the dried wheat grains are placed in the soak solution which is stirred evenly, the ratio of the dried wheat grains to the soak solution is 1:1, the dried wheat grains and the soak solution are mixed and stand for 2-4 days, and the wheat grain fishing bait can be used. The manufacturing method of the wheat grain fishing bait is easy and convenient to implement; the wheat grain fishing bait is soaked in the medicinal liquor and the soak solution respectively, so that the wheat grain fishing bait not only has a cereal wine smell, but also has a fishy smell; a series of nutritional components which are necessary during a fish growing period is added to the wheat grain fishing bait, so that the overall fish inducting effect of the wheat grain fishing bait is ideal, and the wheat grain fishing bait is widely applied to more objects. Meanwhile, because the wheat grains are provided with specific tough skin, small trash fish can be effectively prevented from scrambling for the wheat grain fishing bait, and the phenomenon that small fish larvae are caught by mistakenly eating the wheat grain fishing bait can be effectively prevented.

The invention discloses an antisense oligonucleotide sequence against candida albicans infection and its use, screening an antisense oligonucleotide SEQ ID No:1 and its derivative using the core pseudoknot of candida albicans type I introne ribozyme as the targets, thus repressing the growth of the candida albicans. Test result shows that the antisense oligonucleotide can strongly repress the shearing reactivity of the candida albicans, and can result in the death of the candida albicans cells.

The invention relates to a display information processing system applied to a solar photovoltaic power generation system. The display information processing system comprises a communication module and a display module. The communication module is connected with the solar photovoltaic power generation system, and is used for acquiring a working state parameter of the solar photovoltaic power generation system, and calculating the equivalent amount information of standard coal corresponding to the working state parameter and the amount information of carbon dioxide generated by the combustion of the standard coal corresponding to the amount information. The display module is connected with the communication module, and is used for receiving the working state parameter of the solar photovoltaic power generation system and calculation result information from the communication module, and displaying the working state parameter and the calculation result information in an animation form. Compared with the prior art, the system has the advantages of wide application range, vividness, multifunction and the like.

The invention discloses an aptamer capable of simultaneously recognizing aflatoxins B1, B2, G1 and M1. The aptamer has a nucleotide sequence shown in the formula of SEQ ID NO.1. The end 5' or end 3' of the aptamer is marked with FAM, a mercapto group, FITC or biotin for recognizing aflatoxins B1, B2, G1 and M1. The aptamer can also form an aptamer affinity column. According to the invention, an existing aptamer is used as a main chain, according to the secondary structure, affinity and specificity inspection on the 12 clipped aptamers is carried out by an isothermal titration calorimetric method so that the oligonucleotide aptamer capable of recognizing aflatoxins B1, B2, G1 and M1 with high affinity and high specificity is selected, has the characteristics of high stability, convenience in synthesis, easiness in marking functional groups and the like, and can be widely applied to rapid detection of aflatoxins B1, B2, G1 and M1 in foods.

The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating malignant tumors, a preparation method and a preparation thereof. the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 1-9 parts of ginseng, 1-9 parts of brassica juncea, 1-10 parts of semen raphani, 1-10 parts of radix cynanchi bungei, 1-10 parts of horseradish tree leaves, 1-10 parts of roselle and 1-12 parts of herba cepbalanoplosis segeti. The composition has the advantages of being definite in curative effect, convenient to use, clear in action target and pharmacological effect, wide in raw material source, low in cost, simple in preparation method, high in patient compliance and the like, and can be used for preventing cancers and resisting cancer mutation; the immunity is enhanced; the intestinal microecology is improved, and toxin expelling and cancer prevention are realized.

The invention discloses a method for rapid screening of metabolite peptide aptamers based on molecular docking simulation calculation technology. The method includes (1) determination of target metabolites; (2) obtaining a potential polypeptide library that has a binding force with the target metabolites ; (3) Steps such as molecular docking prediction of potential peptide library and target metabolites. The method is fast, has a wide range of targets, does not require a large amount of manpower and material resources, and has no harm to the experimenter's body, which solves the limiting factors for the widespread application of peptide aptamers at present. The present invention can simplify the procedures of peptide aptamer screening, increase the storage capacity to the greatest extent, and then facilitate the screening of metabolite peptide aptamers with appropriate specificity and affinity, which can be used in the design of detection kits, sensors, etc., and can be used in nutrition detection in the fields of science, biology and clinical diagnosis.

The invention discloses a group of oligonucleotide aptamer capable of specifically recognizing escherichia coli O157:H7 in different growth periods, and belongs to the technical field of food safety biology. A whole cell-SELEX technology is used; the escherichia coli O157:H7 in different growth periods are used as targets; through 14 rounds of repeated screening, appetency and specificity test analysis validation, a group of oligonucleotide aptamer with good appetency and specificity by aiming at escherichia coli O157:H7 in different growth periods is obtained. The aptamer has wide applicationprospects in the aspect of accurately, fast and sensitively detecting the escherichia coli O157:H7 in food.

The invention relates to a formula of an antivirus plant growth regulator preparation for preventing and treating virus diseases of crops and a preparation method thereof. The preparation comprises the main effective components in percentage by weight: 0.1-10% of xanthohumol, 0.1-10% of ningnanmycin, 0.01-1% of oxyenadenine and 0.01-1% of enadenine.

The invention discloses an anti-osteoporosis compound as well as derivatives, a pharmaceutical composition, a preparation method and application thereof. The structure of the compound is shown in a formula (I), the derivatives of the compound relate to an isomer, a diastereoisomer, an enantiomer, a tautomer, a solvate, pharmaceutically acceptable salt or a mixture of the isomer, the diastereoisomer, the enantiomer, the tautomer, the solvate and the pharmaceutically acceptable salt of the compound, and the compound and the derivatives of the compound can inhibit the activity of tartrate-resistant acid phosphatase (TRAP) and influence the expression of osteoclast specificity mRNATRAP, NFATC1, C-FOS and CTSK. The compound has the anti-osteoporosis activity and can be used for preparing anti-osteoporosis medicines, and the synthesis method of the compound is simple, convenient and easy to operate.

The present invention discloses a manufacture method of straw feeds. A commercial straw feed forming biochemical preparation agent prepared by using low-cost chemical raw materials can be used in large, medium and small breeding farms and can also be widely applied for individual breeding farmers. Straws and weeds collected in summer and autumn are processed into winter feeds with good palatability and storage resistance. The manufacture method significantly reduces breeding costs, saves food and improves a situation of human and animal food competition.

The invention discloses a novel omnidirectional bucket loader comprising a tire, an outer rack, an inner bucket, and an outer bucket. A first connecting rod passes through the end part of the outer bucket and the end part of the inner bucket for fixed connection. The first connecting rod, a second connecting rod, and a toothed connecting rod are sequentially connected together in a hinged manner.A rotatable sleeve rod is disposed on the second connecting rod in a sleeved manner, and the two ends of the rotatable sleeve are rotatably connected with the outer rack. A gear is disposed on the toothed connecting rod, and is in a cooperated transmission with the teeth of the toothed connecting rod. The two ends of the gear are respectively fixedly provided with a left circular disc and a rightcircular disc, and a left side and a right side of a horizontal arm are respectively fixedly connected with a thin rod sleeve, and one end of a left thin rope is fixedly connected with a left pedal, and then passes through the left thin rod sleeve, and then is fixedly connected with the upper part of the left circular disc, and one end of the right side thin rope is fixedly connected with a rightpedal, and then passes through the right thin rod sleeve, and then is fixedly connected with the lower part of the right circular disc. The outer rack adopting the tire as a fulcrum is used as a lever, and then the outer bucket can be elevated upwards and downwards, and the outer bucket can be used to move the inner bucket back and forth by a transmission device, and then the omnidirectional moving of the front bucket can be realized.

The invention discloses a novel omnidirectional bucket loader comprising a tire, an outer rack, an inner bucket, and an outer bucket. A first connecting rod passes through the end part of the outer bucket and the end part of the inner bucket for fixed connection. The first connecting rod, a second connecting rod, and a toothed connecting rod are sequentially connected together in a hinged manner.A rotatable sleeve rod is disposed on the second connecting rod in a sleeved manner, and the two ends of the rotatable sleeve are rotatably connected with the outer rack. A gear is disposed on the toothed connecting rod, and is in a cooperated transmission with the teeth of the toothed connecting rod. The two ends of the gear are respectively fixedly provided with a left circular disc and a rightcircular disc, and a left side and a right side of a horizontal arm are respectively fixedly connected with a thin rod sleeve, and one end of a left thin rope is fixedly connected with a left pedal, and then passes through the left thin rod sleeve, and then is fixedly connected with the upper part of the left circular disc, and one end of the right side thin rope is fixedly connected with a rightpedal, and then passes through the right thin rod sleeve, and then is fixedly connected with the lower part of the right circular disc. The outer rack adopting the tire as a fulcrum is used as a lever, and then the outer bucket can be elevated upwards and downwards, and the outer bucket can be used to move the inner bucket back and forth by a transmission device, and then the omnidirectional moving of the front bucket can be realized.

The invention discloses a nucleic acid aptamer that can be used to detect early ovarian cancer tissue and its application in early clinical detection of ovarian cancer. The sequence of the nucleic acid aptamer is selected from any one of SEQ ID NO.1 to SEQ ID NO.8, or the sequence is optimized or modified. Compared with traditional antibodies, the nucleic acid aptamer in the present invention has a wide range of recognized targets and strong specificity, strong affinity, low toxicity and immunogenicity, low cost and time-saving in vitro synthesis, and can be loaded with aptamers Or modified to enhance the functionality of the aptamer and achieve the desired therapeutic goals. Utilizing the short-chain nucleic acid aptamer of the present invention and modifying and marking it, the epithelial cells and tissues of ovarian cancer can be identified and detected, and the process is fast and simple, providing a new idea for early detection and clinical treatment of ovarian cancer.

The invention relates to a display information processing system applied to a solar photovoltaic power generation system. The display information processing system comprises a communication module and a display module. The communication module is connected with the solar photovoltaic power generation system, and is used for acquiring a working state parameter of the solar photovoltaic power generation system, and calculating the equivalent amount information of standard coal corresponding to the working state parameter and the amount information of carbon dioxide generated by the combustion of the standard coal corresponding to the amount information. The display module is connected with the communication module, and is used for receiving the working state parameter of the solar photovoltaic power generation system and calculation result information from the communication module, and displaying the working state parameter and the calculation result information in an animation form. Compared with the prior art, the system has the advantages of wide application range, vividness, multifunction and the like.

The invention discloses an oligonucleotide aptamer for specifically identifying patulin. Through a graphene oxide-based systematic evolution technology of ligands by exponential enrichment, a single stranded oligonucleotide aptamer capable of specifically identifying patulin is acquired. The aptamer can be converted into a report aptamer by marking functional groups and the like. The aptamer is used for detecting patulin. The sequence of the aptamer has a wide application prospect in accurately and quickly detecting patulin in a food.

The invention relates to a device for producing polymeric nanofibers. The device comprises at least one liquid tank (2), a receiving device (7), and a plurality of first electrodes (3) and a plurality of second electrodes (4) forming a potential difference; a substrate in the receiving device passes through the electric field between the first electrodes (3) and the second electrodes (4), or is arranged in the electric field; and the liquid tank (2) reciprocates in a manner of moving relative to the at least one second electrode (4), and in the process that the second electrode (4) is in contact with and / or separated from a polymer solution in the liquid tank (2), the polymer solution dipped by the second electrode (4) is sprayed to the substrate based on the action of the electric field to form a nanofiber layer. The polymer solution can be conveniently smeared on the metal surface by utilizing liquid surface tension, the solution smeared on the metal surface is more uniform, any part of the metal dipped with the polymer solution can be spun, and the spinning efficiency is high.

The invention discloses a preparation method for a CrN coating on a surface of a nuclear zirconium alloy and a product. The sputtering power, the matrix temperature and the bias voltage are adjusted by the multi-target magnetron sputtering technology; and the CrN coating is prepared on the surface of the Zr alloy matrix. According to the invention, the energy of deposited particles is increased byadjusting the bias voltage of the matrix, so the bombardment effect of the deposited particles on the surface of the Zr alloy matrix is enhanced, the bonding at the interface between the CrN coatingand the Zr alloy matrix is promoted, and then the bonding force of the CrN coating and the Zr alloy matrix is increased; the CrN coating with uniform compactness, excellent bonding performance and high thickness and deposited on the surface of the Zr alloy matrix is obtained; the CrN coating is of important scientific significance and engineering application value to a protective coating preparedon the surface of the zirconium alloy and meeting the engineering application thickness (above 10 microns).