Detection method of illegally added dyeing agent 808 scarlet in Dieda Pills Chinese patent medicine
A technology of illegal addition and detection method, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex components, many medicinal flavors, false "positive" interference in the detection process, etc., and achieves good precision, high repeatability, and operation. easy effect
Inactive Publication Date: 2016-03-16
张春辉
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Problems solved by technology
Because Chinese patent medicine is composed of a variety of Chinese medicinal materials according to the corresponding proportion, and processed according to a certain production process, the ingredients contained are much more complicated than a single Chinese medicinal material, and the dosage form of Chinese patent medicine Dieda Wan is Damiwan, not only has a lot of medicinal flavors and complex ingredients in the prescription, but also the dried blood in Dieda Pills only accounts for 3.9% of the prescription, so it is difficult to extract and accurately detect the dye 808 scarlet from Dieda Pills, and the detection process is extremely difficult. Prone to false "positive" interference, which makes it impossible to unambiguously determine results
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[0021] Detailed examples are given below.
[0022] The present invention adopts: instrument and reagent: Shimadzu LC-20AT high performance liquid chromatography system (Japan, Shimadzu company, is equipped with DAD detector); Waters2695 / QuattroMicro liquid chromatography / tandem quadrupole mass spectrometry system (U.S., Waters Company); BP211D electronic balance (Germany, Sartoris Company); AUTOSCIENCEAS10200BT type ultrasonic cleaner (Tianjin Autosaiens Instrument Co., Ltd.). Acetonitrile is a reagent for chromatography, and other reagents are of analytical grade; 808 scarlet reference reagent (National Institute for Food and Drug Control, batch number: 111940-201201).
[0023] Chromatographic conditions: Chromatographic column: Agilent Eclipse Plus C 18 (250×4.6mm, 5μm); using acetonitrile as mobile phase A, 0.02mol L -1 Ammonium acetate solution is mobile phase B, gradient elution (0~15min, 15%B~5%; 15~25min, 5%B); column temperature 30°C; flow rate 1.0mL min -1 ; The de...
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Abstract
The invention relates to a detection method of stain 808 scarlet added illegally in Chinese patent medicine Dieda pill. Specifically, trichloromethane supersonic extraction is adopted, evaporation to dryness by concentration is performed, after methyl alcohol quantitative dissolution, many interference components in the sample are removed and the sample can be smoothly fed to a high performance liquid chromatograph (HPLC) configured with a DAD (Diode Array Detector) detector, therefore, a trace of detection component 808 scarlet can be extracted from a big honeyed pill comprising extremely complex components, and can be detected smoothly and accurately. An HPLC-DAD method is used for qualitatively and quantitatively detecting the stain 808 scarlet added illegally in Chinese patent medicine Dieda pill. By the detection method, problems of much interference during extraction and detection of the 808 scarlet caused by big prescription and complex components of the Dieda pill product are solved effectively, and the detection method has the characteristics of being simple and convenient to operate, excellent in precision and high in repeatability, and is expected to be a standard method for quality control on the Chinese patent medicine Dieda pill.
Description
technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a detection method for illegally adding dyeing agent 808 scarlet in Dieda Pills Chinese patent medicine. Background technique [0002] Dieda pills are composed of Panax notoginseng, angelica, white peony root, red peony root, peach kernel, safflower, dried blood, northern liu jinu, hot bone drynaria, diaphan, sumac, peony bark, frankincense (made), myrrh (made), turmeric, vinegar three-lens, windproof, melon seeds, citrus aurantium (stir-fried), bellflower, licorice, akebia, calcined natural copper, and wood beetle, a large prescription Chinese medicine preparation composed of 24 Chinese herbal pieces. It has the effects of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain. It is used for bruises, broken tendons and fractures, blood stasis, swelling and pain, flashbacks and other symptoms. The preparatio...
Claims
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IPC IPC(8): G01N30/02
Inventor 张春辉吴爱英李新荣楚敏卢京光
Owner 张春辉