Screening purification and scale breeding method for thermocyclops
A technology of warm sword Daphnia and cultivation method, applied in the direction of climate change adaptation, fish farming, application, etc., can solve the problems of staying in natural reproduction, etc., and achieve the effects of moderate size, simple operation and good quality.
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[0023] A method for screening, purification and large-scale cultivation of Daphnia vulgaris, capturing copepods from estuaries or breeding ponds at the junction of brackish water and freshwater; under set conditions, directional screening of Daphnia vulgaris is carried out; the cultivation of Daphnia vulgaris is optimized To carry out large-scale continuous cultivation under conditions, the specific steps are as follows:
[0024] (1) Germplasm acquisition: In the estuary or breeding pond at the junction of brackish water and fresh water, when the water temperature is 18-22℃ (usually the transition of spring and summer or late autumn), select the area with the specific gravity of water between 1.005-1.012 and use 120 mesh A trawl or fishing net made of sieve tulle, harvesting copepods at the surface of a water body of 30-50cm as a source of germplasm for screening;
[0025] (2) Directional screening: After cultivating the harvested copepods at a temperature of 2...
Example Embodiment
[0028] Example 1
[0029] A method for screening, purifying, and large-scale cultivation of Daphnia vulgaris, the specific steps are as follows:
[0030] (1) Germplasm acquisition: in the estuary or breeding pond at the junction of saltwater and fresh water, at a water temperature of 20℃, select an area with a water specific gravity of 1.008, and use a trawl or fishing net made of 120-mesh sieve tulle at the surface of a 40cm water body. Harvest the copepod population as a germplasm source for selection;
[0031] (2) Targeted screening: After cultivating the harvested copepod population at a temperature of 20°C, a salinity of 10, and a light intensity of 90 Lux for 6 days, raise the water temperature to 24°C and the salinity to drop to 8. After incubating for 4 days, Then return to the initial incubation condition temperature 20℃, salinity 10, light intensity 90 Lux and incubate for 6 days, that is to complete a cycle of directional screening, after completing at least one cycle of...
Example Embodiment
[0034] Example 2
[0035] Same as the above embodiment 1, the difference lies in:
[0036] In step (1), at the estuary at the junction of brackish and fresh water, at a water temperature of 18℃, select an area with a specific gravity of 1.005, use a trawl or fishing net made of 120-mesh sieve tulle, and harvest copepods at the surface layer of a 30cm water body Population as a germplasm source for screening;
[0037] In step (2), the harvested copepod population is cultivated for 7 days under the conditions of temperature 18℃, salinity 7 and light intensity 30 Lux, then raise the water temperature to 22℃, and the salinity drops to 7, and after incubating for 5 days, it will recover. Incubate for 7 days at the initial cultivation condition temperature of 18℃, salinity 7, and light intensity of 30 Lux, and then complete one cycle of directional screening. After completing at least one cycle of directional screening, the purebred E. occidentalis will be obtained. After incubating for...
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