Application of irisin in preparation of antihyperglycemic medicines

An irisin and anti-diabetic technology, applied in the field of medicine, can solve the problems such as the anti-diabetic drug effect of irisin that has not been reported, and achieve the effect of broadening the selection field.

Active Publication Date: 2015-05-20
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of irisin in preparation of antihyperglycemic medicines
  • Application of irisin in preparation of antihyperglycemic medicines
  • Application of irisin in preparation of antihyperglycemic medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 The making of diabetes model

[0017] Six-week-old C57BL / 6J mice were randomly divided into three groups:

[0018] (1) Normal mice-physiological saline group (Ctrl-Saline): as the normal control group, they were given a normal diet (non-high-fat diet) all the time, and after 8 weeks, they were given subcutaneous infusion of saline (saline) for 2 weeks as a control of irisin ;

[0019] (2) Diabetes-saline group (STZ / HFD-Saline): Diabetes was induced by streptozotocin (STZ) combined with high-fat diet (HFD), and after 8 weeks, normal saline (saline) was subcutaneously infused for 2 weeks as irisin contrast;

[0020] (3) Diabetes-irisin group (STZ / HFD-Irisin): Diabetes was induced by Streptozotocin (STZ) combined with high-fat diet (HFD), and irisin was subcutaneously infused for 2 weeks after 8 weeks.

[0021] Diabetes model making method: The diabetes model is induced by combined application of small doses of STZ and high-fat diet, which is a recognized...

Embodiment 2

[0022] Example 2 Subcutaneous continuous administration of micro-osmotic pump

[0023] Under pentobarbital sodium anesthesia and aseptic operation, a miniature osmotic pressure pump (1002 type, Alzet company) containing normal saline or irisin was implanted into the subcutaneous tissue of the back of the mouse neck, the incision was sutured, and penicillin was injected intramuscularly every day after the operation 3 days in a row to prevent infection. The infusion dose of irisin was 1.44 nmol / day (0.24 nmol / μl) for 2 consecutive weeks.

Embodiment 3

[0024] Example 3 Glucose Tolerance Test (GTT) and Insulin Tolerance Test (ITT)

[0025] Glucose tolerance test (GTT): After the mice were fasted overnight, glucose (2.0 g / kg) was injected intraperitoneally, and blood glucose was measured before and 15, 30, 60 and 120 min after the injection.

[0026] Insulin tolerance test (insulin tolerance test, ITT): mice were fasted for 6 hours, and insulin (0.75 units / kg) was injected intraperitoneally, and blood glucose was measured before and 15, 30, 60 and 120 minutes after injection.

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Abstract

The invention discloses a method for providing a new intervention target for preparation of antihyperglycemic medicines and using the irisin into development of diabetes-related medicines to prepare relatively good medicines for treating diabetes. After STZ/HFD-induced mice with diabetes are subjected to subcutaneous injection with irisin, the fasting serum glucose level is significantly reduced; the insulin sensitivity is significantly improved; by irisin, gluconeogenesis is reduced and glycogen synthesis is increased; the application proves that irisin is capable of significantly improving glucose metabolic disorder and insulin resistance in diabetes, is an effective target for treating diabetes, and can be developed as a novel antihyperglycemic medicine or medicine target; and a new way and means are provided for detection and treatment of diabetes.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of irisin in the preparation of antidiabetic drugs. Background technique [0002] Diabetes Mellitus (DM) is a series of systemic metabolic disorders caused by relative or absolute insulin deficiency, or decreased sensitivity of target cells to insulin. According to the cause of the disease and the distribution in different populations, diabetes is often divided into type I diabetes and type II diabetes clinically. Diabetes is a common clinical endocrine and metabolic disease, and another chronic disease that seriously endangers human health after cardiovascular and cerebrovascular diseases and tumors. At present, the incidence of diabetes is on the rise worldwide, among which type II diabetes is the majority. In my country, type II diabetes accounts for about 95% of diabetic patients, and there is a tendency to expand and rejuvenate. [0003] Type II diabetes, also known...

Claims

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Application Information

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IPC IPC(8): A61K38/22A61P3/10
Inventor 朱国庆熊晓青刘桐言
Owner NANJING MEDICAL UNIV
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