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Preparation method and application of proanthocyanidin extract in grapes

A technology of anthocyanin extract and proanthocyanidin, which is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of differences in extraction rates of different components, high operation and maintenance costs, and achieve the effect of reducing toxic and side effects

Active Publication Date: 2015-05-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires specific equipment, the equipment operation and maintenance costs are high, and the extraction rate of different components is very different

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of grape skins and grape pulp

[0033] 1) Select fresh grapes (purchased in the market), rinse with tap water and then rinse with deionized water, and then remove the grape seeds from the grapes.

[0034] 2) Put the grape fruit with the grape seeds removed in a stainless steel container, and then mash it as thoroughly as possible with an instrument.

[0035] 2. Add mixed organic solvent to extract proanthocyanidins

[0036] 1) Add methanol, ethanol and aqueous solution according to the volume ratio of 1:1:1, fully stir and mix the organic solvent and the crushed seedless grapes.

[0037] 2) Treat after standing for 24 hours.

[0038] 3. Negative pressure freeze-thaw

[0039] 1) After standing still, carry out suction filtration treatment to carry out solid-liquid separation.

[0040] 2) Under the condition of negative pressure of -0.10mPa, freeze and dissolve with 0°C cold water, recover methanol and ethanol solvent by evaporation, and concentrate the...

Embodiment 2

[0067] The specific preparation method is basically the same as that of Example 1, except that:

[0068] In step 2, methanol, ethanol and an aqueous solution are prepared into a mixed solution in a volume ratio of 0.5:1:2, and in step 3, a negative pressure freeze-thaw is performed under the condition of -0.30 mPa. During microwave-assisted extraction of proanthocyanidins in step 4, firstly, microwave treatment was performed under the condition of 550W microwave for 15 minutes, and the microwave was set to break multiple times in a short time. The microwave time was 0.8s each time, and the interval time was 0.5s. During this period, if the temperature of the reaction solution exceeds the set alarm temperature (45° C.), the microwave is stopped. Finally, during centrifugation, the microwave-treated extract was centrifuged at 2000 r / min for 20 min.

[0069] The proanthocyanidins prepared in Example 2 were used in the following animal experiments

[0070] Fifty Kunming mice (ea...

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Abstract

The invention discloses a preparation method of an proanthocyanidin extract in grapes, which comprises the following steps: (1) preparation of grape skin and grape flesh: removing grape seeds from grapes to obtain grape skin and grape flesh; (2) extraction of proanthocyanidin: preparing a mixed solution composed of methanol, ethanol and a water solution, mixing the mixed solution with the grape skin and grape flesh prepared in the step (1), and reacting; (3) carrying out solid-liquid separation on the reaction product to obtain an extracting solution, carrying out freeze thawing on the extracting solution under negative pressure, and concentrating; (4) microwave extraction of proanthocyanidin: treating the extracting solution under the conditions of 550W microwaves, and centrifugating; and (5) concentration: concentrating the centrifugated supernate to obtain the concentrated proanthocyanidin extract. The method has higher extraction efficiency; and the extracted proanthocyanidin compound has the effects of resisting aging and the like, and is applicable to antiaging beauty treatment industry.

Description

technical field [0001] The invention belongs to the field of biological beauty, and in particular relates to a preparation method and application of proanthocyanidin extract in grape fruit. Background technique [0002] Grape, also known as grape, is a common deciduous woody vine of the genus Vitis. It is a fruit substance with extremely high nutritional value in berry fruits, native to western Asia. "Shen Nong's Materia Medica" stated that grapes have the effect of "eating for a long time, not getting old and prolonging life", and grapes are a kind of fruit with anti-anemia, anti-inflammatory and beauty benefits. Grape contains anthocyanins and other polyphenolic chemical components. These components have outstanding antioxidant properties, which can eliminate free radicals in the human body, inhibit lipid peroxidation in the body, and enhance skin immunity, prevent ultraviolet rays, whiten, freckle and reduce Wrinkle-reducing, pore-reducing and moisturizing effects. At p...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61K8/49A61Q19/08
CPCA61K8/022A61K8/498A61Q19/08C07D311/62
Inventor 陈海佳王一飞葛啸虎池诗源王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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