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Protein GmSqm associated with insect tolerance of plants as well as coding gene and application thereof

A coding gene and plant technology, applied in the field of plant insect resistance-related protein GmSqm and its coding gene and application, to achieve the effect of reducing insect weight and body length

Inactive Publication Date: 2015-05-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although transgenic crops expressing Bt toxin can effectively resist pests, further research is still needed to develop more insect-resistant genes, and the research and cloning of insect-resistant related genes is of great significance for the cultivation of new insect-resistant soybean varieties

Method used

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  • Protein GmSqm associated with insect tolerance of plants as well as coding gene and application thereof
  • Protein GmSqm associated with insect tolerance of plants as well as coding gene and application thereof
  • Protein GmSqm associated with insect tolerance of plants as well as coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the acquisition of GmSqm protein and its coding gene

[0050] 1. When the wild soybean ED059 (Glycine soja Sieb.et Zucc.) is cultivated to the third three primary compound leaves under normal conditions, the third instar cotton bollworm larvae are used for treatment on the second fully developed compound leaves.

[0051] 2. After the cotton bollworm was treated for 24 hours, the treated leaves were harvested, total RNA was extracted, and cDNA was obtained by reverse transcription; using the cDNA as a template, PCR amplification was performed using primers PF and primer PR to obtain a PCR amplification product of 1590 bp. The sequences of the above primers are as follows:

[0052] Primer PF: 5'-ATGATGGGTTATGAGTATATTTTGG-3;

[0053] Primer PR: 5'-TTAATCTTCCAAATTGGTAGGGGGT-3.

[0054] 3. The PCR amplification product was recovered and cloned into pGEM-T vector to obtain the recombinant vector, which was transformed into Escherichia coli DH5α and then sent f...

Embodiment 2

[0056] Embodiment 2, the acquisition of transgenic GmSqm Arabidopsis thaliana and its phenotypic identification

[0057] 1. Construction of plant expression vectors

[0058] 1. When the wild soybean ED059 (Glycine soja Sieb.et Zucc.) is cultivated to the third three primary compound leaves under normal conditions, the third instar cotton bollworm larvae are used for treatment on the second fully developed compound leaves.

[0059] 2. After the cotton bollworm was treated for 24 hours, the treated leaves were harvested, the total RNA was extracted, and cDNA was obtained by reverse transcription; using the cDNA as a template, PCR amplification was performed using primers PF and primer PR to obtain a PCR amplification of 1590 bp in size product. The sequences of the above primers are as follows: primer PF: 5'-ATGATGGGTTATGAGTATATTTTGG-3; primer PR: 5'-TTAATCTTCCAAATTGGTAGGGGGT-3.

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Abstract

The invention discloses a protein GmSqm associated with the insect tolerance of plants as well as a coding gene and application thereof. The protein is the following a) or b): a) a protein with an amino acid sequence shown in a sequence 1 in a sequence table; b) a protein which is obtained by carrying out substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence shown in the sequence 1 in the sequence table and is associated with the insect tolerance of plants. Experiments prove that compared with wild arabidopsis thaliana plants and trans-empty vector arabidopsis thaliana plants, trans-GmSqm arabidopsis thaliana plants have the advantages that after newly-hatched helicoverpa armigera Hubner larvae are fed with the trans-GmSqm arabidopsis thaliana plants, the weights of the helicoverpa armigera Hubner larvae are obviously reduced, and the body length of the helicoverpa armigera Hubner larvae is obviously reduced. The protein GmSqm is of great importance in cultivating plants tolerant to insects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a protein GmSqm related to plant insect resistance, its coding gene and application. Background technique [0002] In the next 40 years, the world population will reach 10 billion. Under such circumstances, the primary task of agriculture is to maximize the production of food and other products on the premise of environmental sustainability and cost savings, while a major factor limiting food production is due to insect infestation, which is estimated to cause 10-20% crop loss. Soybean is the world's major oil crop, which is the main source of vegetable fat and protein. In soybean production, the yield and quality of soybean are often harmed by herbivorous insects. However, soybean yield often suffers serious losses due to the feeding of soybean leaves by herbivorous insects. Cotton bollworm (Helicoverpa armigera Hübner), belonging to the Noctuidae family of Lepidopter...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8286
Inventor 王笑一陈海峰张婵娟单志慧陈李淼陈水莲张晓娟周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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