A detection method of four flavonoid active ingredients in Chuankezhi injection
A detection method and technology for active ingredients, applied in the field of medicine, can solve the problems of poor quality control, complex ingredients, and many sources of Epimedium medicinal materials.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0107] (1) Preparation of reference solution: about 5 mg each of epimedin A, epimedin B, epimedin C and icariin, accurately weighed, respectively placed in 5ml measuring bottles, dissolved in methanol and diluted to Scale, prepared into 4 kinds of reference substance stock solution. Accurately measure the appropriate amount of the 4 flavonoid reference substance stock solutions respectively, put them in a 10ml measuring bottle, add methanol to dilute to the mark, shake well, and obtain a mixed reference substance stock solution.
[0108] (2) Preparation of internal standard solution: Take about 10 mg of naringin reference substance, weigh it accurately, place it in a 10 ml measuring bottle, add methanol to dissolve and dilute to the mark, and prepare internal standard stock solution. Precisely measure an appropriate amount of internal standard stock solution, put it in a 10ml measuring bottle, add acetonitrile: 0.1% formic acid aqueous solution (35:65) to dilute to the mark, s...
Embodiment 2
[0117] (1) Preparation of reference solution: about 5 mg each of epimedin A, epimedin B, epimedin C and icariin, accurately weighed, respectively placed in 5ml measuring bottles, dissolved in methanol and diluted to Scale, prepared into 4 kinds of reference substance stock solution. Accurately measure appropriate amounts of 4 kinds of flavonoid reference substance stock solutions respectively, put them in a 10ml measuring bottle, add acetonitrile: 0.1% formic acid aqueous solution (65:35) to dilute to the mark, shake well, and obtain a mixed reference substance stock solution.
[0118] (2) Preparation of internal standard solution: Take about 10 mg of naringin reference substance, weigh it accurately, place it in a 10 ml measuring bottle, add methanol to dissolve and dilute to the mark, and prepare internal standard stock solution. Precisely measure an appropriate amount of internal standard stock solution, put it in a 10ml measuring bottle, add acetonitrile: 0.1% formic acid ...
Embodiment 3
[0127] (1) Preparation of reference solution: about 5 mg each of epimedin A, epimedin B, epimedin C and icariin, accurately weighed, respectively placed in 5ml measuring bottles, dissolved in methanol and diluted to Scale, prepared into 4 kinds of reference substance stock solution. Accurately measure appropriate amounts of 4 kinds of flavonoid reference substance stock solutions respectively, put them in a 10ml measuring bottle, add acetonitrile: 0.1% formic acid aqueous solution (65:35) to dilute to the mark, shake well, and obtain a mixed reference substance stock solution.
[0128] (2) Preparation of internal standard solution: Take about 10 mg of naringin reference substance, weigh it accurately, place it in a 10 ml measuring bottle, add methanol to dissolve and dilute to the mark, and prepare internal standard stock solution. Precisely measure an appropriate amount of internal standard stock solution, put it in a 10ml measuring bottle, add acetonitrile: 0.1% formic acid ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More