Extraction method of active ingredients of medicinal fungi

An extraction method and technology of active ingredients, which are applied in the field of food preparation or processing, can solve the problems of low extraction rate of active ingredients, long enzymolysis reaction time, and destruction of active substances, and achieve shortened extraction time, low cost, and small damage Effect

Inactive Publication Date: 2015-05-27
BEIJING FUYUAN GLOBAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of high energy consumption, long enzymatic hydrolysis reaction time, low extraction rate of acti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting active ingredients of medicinal fungi, comprising the following steps:

[0025] (1) 1 kg of medicinal fungus raw material is crushed, and the diameter after crushing is 75 μm to 300 μm;

[0026] (2) Add enzymes to the crushed medicinal fungus raw materials for enzymolysis, the amount of enzymes added is 1-2% of the total reaction system, and enzyme activators are added at the same time, the temperature of the extraction process is 20°C-45°C, and the pH value 4-9, the enzymatic hydrolysis reaction is carried out for 40min-100min;

[0027] (3) Filter the product obtained after enzymolysis, collect the extract, and obtain the active ingredient of the medicinal fungus.

[0028] The medicinal fungus in this example is Phellinus japonica, each 1% of cellulase and pectinase is added, and the enzyme activator is metal ion, such as Mg 2+ 、Na + or Ca 2+ , the amount added is 0.1% of the enzyme amount. 2-5 times of water can be added to the reaction sy...

Embodiment 2

[0035] The medicinal bacterium in the present embodiment is Hericium erinaceus, adding enzyme is cellulase and pectinase, enzyme amount is 1%, can also select cellulase, hemicellulase, pectinase, pepsin, Any two or more of papain, bromelain, ficin, glucoamylase, α-amylase, β-amylase, and isoamylase are extracted, and the amount of mixed enzymes is 2% of the medicinal fungus raw material. It can be 1% or 3%. The enzyme activator is a metal ion, such as Mg 2+ 、Na + or Ca 2+ , the added amount is 0.2% of the enzyme amount, also can be 0.1%, 0.3% or 0.4%.

[0036] The extraction method of this example extracts 75% active ingredients, up to 90%, wherein the content of triterpenes and adenosine is twice that of the traditional enzyme extraction method, and the yields of triterpenes and adenosine are detected by published experimental methods.

[0037]The extraction method and conditions in this embodiment are the same as those in Embodiment 1 except those described in this embod...

Embodiment 3

[0039] The medicinal fungus in this embodiment is Polyporus umbilicalis, selects cellulase, hemicellulase and pectinase to extract, and the amount of mixed enzyme is 3% of the medicinal fungus raw material, also can be 2% or 1%; Any two or two of cellulase, hemicellulase, pectinase, pepsin, papain, bromelain, ficin, glucoamylase, α-amylase, β-amylase, and isoamylase can be selected. More than two kinds are extracted, and the amount of mixed enzyme is 1%, 2% or 3% of the medicinal fungus raw material. The enzyme activator is a metal ion, such as Mg 2+ 、Na + or Ca 2+ , the added amount is 0.2% of the enzyme amount, also can be 0.1%, 0.3% or 0.4%.

[0040] The extraction method of this example extracts 75% active ingredients, up to 90%, wherein the content of triterpenes and adenosine is twice that of the traditional enzyme extraction method, and the yields of triterpenes and adenosine are detected by published experimental methods.

[0041] The extraction method and conditio...

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Abstract

The invention provides an extraction method of active ingredients of medicinal fungi. The extraction method comprises the following steps: (1) crushing raw materials of the medicinal fungi, wherein the diameters are 75-300 microns after the raw materials are crushed; (2) adding mixed enzymes and an enzyme activator into the crushed raw materials of the medicinal fungi to implement enzymolysis, wherein the dosage of the mixed enzymes is 1-3% of the raw materials of the medicinal fungi, the enzymolysis temperature is 20-45 DEG C, the pH value is 4-9, and enzymolysis reaction is carried out for 40-100 minutes; and (3) filtering the product subjected to enzymolysis and collecting extraction liquid to obtain the active ingredients of medicinal fungi. According to the extraction method provided by the invention, the yield is high, the energy consumption is low and the damage on the active ingredients of the medicinal fungi is alleviated, and the extraction time is remarkably shortened compared with that of the existing extraction method, and the content of triterpene and adenosine in the extract is improved to a greater extent compared with that of the existing extraction method, moreover, the operation is simple and the cost is relatively low.

Description

technical field [0001] The invention belongs to the technical field of food preparation or treatment, and in particular relates to a method for extracting active ingredients of medicinal fungi. Background technique [0002] Medicinal fungi contain active ingredients and are a class of fungi with pharmacological effects. Modern medical research shows that medicinal fungi not only have the traditional meaning of health preservation, but also have the effect of enhancing immunity and anti-tumor. In addition, medicinal bacteria have no toxic and side effects while exerting their effects, and many of them can be used as ordinary dishes. With the deepening of scientific research, medicinal fungi have become a seed industry with unlimited development potential. Through nearly 20 years of research, the active ingredients in medicinal fungi have been identified as fungal polysaccharides, adenosine, steroids, saponins, three Terpenoids, amino acids, ganoderma acid, aranolic acid, et...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L31/00
CPCA23V2002/00A23V2250/21
Inventor 郁实
Owner BEIJING FUYUAN GLOBAL BIOTECH
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