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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and probiotics, applied in application, bacteria, microorganism and other directions, can solve the problems of insufficient bile salt tolerance, loss and other problems, and achieve the effect of good tolerance

Active Publication Date: 2015-05-27
BEIJING BOJINYUAN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the bacteria are not resistant to acid and bile salts, they cannot enter the intestinal tract and lose their function

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
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Embodiment Construction

[0022] The example is: this Lactobacillus plantarum, it is Lactobacillus plantarum BJYL132, the preservation number is CGMCCNo.9646, the preservation date is September 11, 2014, the preservation unit: China Microorganism Culture Collection Management Committee Ordinary Microorganism Center .

[0023] The isolation and identification methods of Lactobacillus plantarum BJYL132 are as follows:

[0024] 1. Separation method

[0025] Weigh 5g of the sample (naturally fermented food) into 45ml sterile buffered saline solution (add an appropriate amount of glass beads) as the mother solution, shake for 10 minutes and mix well, make gradient dilution with sterile buffered saline solution, select three suitable gradients, and draw 0.1ml was streaked in MRS medium, and each dilution gradient was repeated twice. The culture dish was placed in a 37°C incubator for 48-72 hours, and smooth, convex circles, neat edges, white or milky white colonies were selected. microscopic examination. ...

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Abstract

The invention relates to a lactobacillus plantarum and an application thereof. The lactobacillus plantarum is lactobacillus plantarum BJYL132 which is collected on September 11, 2014, in the collection unit China General Microbiological Culture Collection Center, with the collection number CGMCC No. 9646. The lactobacillus plantarum BJYL132 is derived from the naturally fermented food, can be colonized in a human body or an animal body, and is tolerant to gastric acid and cholate; meanwhile, the lactobacillus plantarum has a good inhibition effect on the escherichia coli and is capable of surviving at a room temperature for 30 days; the bacterial strain has a good synergistic growth effect with other types of lactic acid bacteria, can be applied to animal feed additives and bacteriostats, and aquaculture, and also can be widely applied to the fields of other food and biological products.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a plant lactobacillus and application thereof. Background technique [0002] Lactobacillus plantarum is closely related to the lives of humans and animals. It is a lactic acid bacteria commonly found in cream, meat, many vegetable fermented products, and silage. It can pass through the stomach and colonize the intestinal tract to play a beneficial role, such as maintaining intestinal bacteria It has many functions, such as the balance of group, promoting the absorption of nutrients, alleviating lactose intolerance and inhibiting the formation of tumor cells. At present, there are many application fields including food, feed, medical care, cosmetics, beauty, aquaculture, industry and agriculture. production, biological preservatives, probiotic preparations, etc. [0003] Multiple physiological functions of Lactobacillus plantarum as a nitrogen source for the host. However,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/25
CPCC12N1/205C12R2001/25
Inventor 刘英华姜波刘金华
Owner BEIJING BOJINYUAN BIOTECH
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