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Method of extracting DHA and EPA in type of triglyceride from deep-sea fish

A technology of triglyceride and fatty acid triglyceride, which is applied in the field of biopharmaceuticals, can solve the problems of high acid value, high cost, and need decolorization for deep color, and achieves the effect of low acid value and low production cost.

Active Publication Date: 2015-05-27
SHENZHEN HAIYOUKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting triglyceride type DHA and EPA from deep-sea fish, aiming to solve the problem that the DHA and EPA extracted from fish oil in the prior art have low purity, high acid value, deep color and need decolorization, and Obtaining DHA and EPA as mixtures such as monoglycerides and triglycerides of DHA and EPA or ethyl ester fish oil and the problem of high cost

Method used

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  • Method of extracting DHA and EPA in type of triglyceride from deep-sea fish
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  • Method of extracting DHA and EPA in type of triglyceride from deep-sea fish

Examples

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Effect test

Embodiment 1

[0050] S11. Preparation of Crude Fish Oil

[0051]The crude fish oil of deep-sea fish was prepared by enzymatic hydrolysis technology. The deep-sea fish is crushed by a bone crusher to form a minced shape, weigh 600g of meat pulp, add water to seal it according to the ratio of meat:water=0.5:1 (m:m), and cook it at 85°C for 1 hour in a dark room under nitrogen. . The pH was adjusted to 8.0 using NaOH. Add trypsin with a mass fraction of 2%, and perform enzymatic hydrolysis in a dark room at room temperature (25°C) for 17 hours. After being centrifuged at 5000r / min for 20min, the enzymatic hydrolysis liquid in the upper layer was taken, which was the crude fish oil.

[0052] S12. Preparation of free fatty acids

[0053] Enzyme hydrolysis technology is used to hydrolyze the fish oil glyceride form of crude fish oil to obtain free fatty acids. Using lipase 1LVK-F100 as a catalyst, the enzyme dosage is 2%, the reaction temperature is 50°C, and the reaction time is 24h to obta...

Embodiment 2

[0060] S21. Preparation of Crude Fish Oil

[0061] The crude fish oil of deep-sea fish was prepared by enzymatic hydrolysis technology. The deep-sea fish is crushed by a bone crusher to form a minced shape. Weigh 600g of meat slurry, add water to seal it according to the ratio of meat:water=0.5:1 (w:w), and cook it at 85°C for 0.5 h. The pH was adjusted to 8.0 using NaOH. Add trypsin with a mass fraction of 1.5%, and perform enzymatic hydrolysis in a dark room at room temperature (20°C) for 15 hours. After being centrifuged at 4500r / min for 20min, the enzymatic hydrolysis solution in the upper layer was taken, which was the crude fish oil.

[0062] S22. Preparation of free fatty acid

[0063] Enzyme hydrolysis technology is used to hydrolyze the fish oil glyceride form of crude fish oil to obtain free fatty acids. Using lipase 1LVK-F100 as a catalyst, the enzyme dosage is 1.5%, the reaction temperature is 45°C, and the reaction time is 20h to obtain free fatty acids.

[...

Embodiment 3

[0070] S31. Preparation of Crude Fish Oil

[0071] The crude fish oil of deep-sea fish was prepared by enzymatic hydrolysis technology. The deep-sea fish is crushed by a bone crusher to form a minced shape, weigh 600g of meat pulp, add water to seal it according to the ratio of meat: water = 0.5:1.5 (m:m), and cook it at 90°C for 1 hour in a dark room with nitrogen. . The pH was adjusted to 8.0 using NaOH. Add trypsin with a mass fraction of 3%, and perform enzymatic hydrolysis in a dark room at 30°C for 15 hours. After being centrifuged at 6000r / min for 20min, the enzymatic hydrolysis solution in the upper layer was taken, which was the crude fish oil. Crude fish oil was tested according to the national standard GB / T17377-2008 "Gas Chromatographic Analysis of Fatty Acid Methyl Ester of Animal and Vegetable Oils".

[0072] S32. Preparation of free fatty acid

[0073] Enzyme hydrolysis technology is used to hydrolyze the fish oil glyceride form of crude fish oil to obtain ...

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Abstract

The invention belongs to the technical field of biopharmacy and provides a method of extracting DHA and EPA in the type of triglyceride from deep-sea fish. The method includes steps of: preparing crude fish oil with enzymolysis technology, preparing free fatty acid, separating poly-unsaturated fatty acids containing the EPA and the DHA through a urea-embedding method, and preparing the fatty acid triglyceride of the DHA and the EPA. The method can effectively extract and enrich the DNA and the EPA from the deep-sea fish. The obtained crude fish oil is low in acidic value and is high in hydrolysis degree of esterified fatty acids. The finally products of the DHA and the EPA are in the type of triglyceride with the contents of the DHA and the EPA are in the type of triglyceride being high.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a method for extracting triglyceride-type DHA and EPA from deep-sea fish. Background technique [0002] Deep-sea fish, such as tuna, is a large-scale pelagic important commercial food fish. Because deep-sea fish must keep swimming quickly to maintain the body's supply, and only move in the deep sea, the meat is tender and delicious, and it is not polluted by the environment. It is a rare healthy delicacy for modern people. The deep-sea fish processing industry has become an important industry, but there are a series of major problems in the process of deep-sea fish processing, such as difficult quality control, low utilization of scraps, high production energy consumption, and serious loss of raw materials, resulting in large economic losses for enterprises. Therefore, the leftovers generated in the deep-sea fish processing process can increase operating income and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6472Y02P20/54
Inventor 陈丽娟王践云窦庶华杜丽娟钟舟
Owner SHENZHEN HAIYOUKANG BIOTECH CO LTD
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