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Fast testing card for organophosphorus and carbamate pesticide residues

A carbamate and pesticide residue technology, applied in the field of bioengineering, can solve problems such as poor anti-interference ability, long detection time, and insufficient response, and achieve strong anti-interference ability, short detection time, and avoid artificial folding.

Active Publication Date: 2015-05-27
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of use will cause many potential hazards, especially the food poisoning caused by organophosphorus pesticide residues in vegetables is very serious
However, the existing rapid test cards for rapid detection of pesticide residues all have problems such as relying on manual folding, insufficient response, long detection time, and poor anti-interference ability.

Method used

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  • Fast testing card for organophosphorus and carbamate pesticide residues
  • Fast testing card for organophosphorus and carbamate pesticide residues
  • Fast testing card for organophosphorus and carbamate pesticide residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preferred embodiment 1 of the present invention: paste and fix the climbing film 14 that is fixed with indophenol acetate on the bottom plate 11, the size of the climbing film 14 is 10 mm long × 5 mm wide, and the amount fixed with indophenol acetate is 0.3 mg; One end of the sample film 14 is pasted with a color-developing pad 15 and a water-absorbing pad 16. The material of the color-developing pad 15 is synthetic cellulose paper, and the size is 7 mm long x 5 mm wide. The water-absorbing pad 16 is 20 mm long x 5 mm wide. The membrane 13 and the enzyme pad 12 on which the cholinesterase is fixed are pasted successively. The size of the diaphragm 13 is 3 mm in length x 5 mm in width, the size of the enzyme pad 12 is 20 mm in length x 5 mm in width, and the amount of immobilized cholinesterase contained therein is 0.5 U. The speed test paper 1 is placed in the groove of the plastic lower cover 2, and the base plate 11 is PVC base plate paper.

[0044] The steps to dete...

Embodiment 2

[0047] Preferred embodiment 2 of the present invention: the amount of immobilized cholinesterase contained in the enzyme pad 12 is 2 U, the amount of immobilized indophenol acetate contained in the climbing membrane 14 is 3.0 mg, and the material of the diaphragm 13 is filter paper.

[0048] The steps to detect pesticide residues are as follows:

[0049] Step 1. Select pure water for the blank sample solution, place the detector horizontally, add 0.1mL pure water to the sample injection hole 34, react at 37°C for 7 minutes, and observe the color development results. Dark blue is observed from the observation hole 33, indicating that There are no pesticide residues in the sample solution.

[0050] Step 2. Prepare a simulated sample. The simulated sample is a pakchoi sample spiked with trichlorfon to 0.2mg / kg. The processing steps of the vegetable simulated sample are: take 5g fresh weight vegetable sample and cut it into pieces about 1cm×1cm in size. Put it in a sample cup, th...

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Abstract

The invention discloses a fast testing card for organophosphorus and carbamate pesticide residues. The fast testing card comprises a lower cover and an upper cover buckled with the lower cover, wherein the upper cover is provided with a sample adding hole and an observation hole, rapid testing paper for testing the pesticide residues is fixed in the lower cover, the rapid testing paper comprises a bottom plate and a sample climbing film adhered to and fixed with the middle of the bottom plate, a color development pad and a water absorption pad are sequentially adhered to one end of the sample climbing film, a separation film and an enzyme pad are sequentially adhered to the other end of the sample climbing film, the position of the observation hole corresponds to an observation position of the color development pad, the position of the sample adding hole corresponds to the position of the enzyme pad, the detection time is short, the operation is simple, the anti-interference ability is strong due to lateral chromatography, the color development effect is good, the fast testing card can be widely used for quickly detecting the organophosphorus and carbamate pesticide residues, and is applicable to the technical field of bioengineering.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a quick test card for organophosphorus and carbamate pesticide residues. Background technique [0002] At present, pesticides have made great contributions to ensuring high agricultural yields, but they are easy to remain in the environment, especially pesticides and their degradation products in food, which also bring potential harm to humans. Pesticides entering crops will cause food pollution and endanger human health. , symptoms such as visceral necrosis, sclerosis, decreased immune function, and dysplasia will appear. According to statistics from the Food and Agriculture Organization of the United Nations (FAO), if pesticides are not used, the annual average annual losses caused by diseases, insects, and weeds in agriculture around the world account for about 37% of the total crop output, and the annual loss is as high as 126 billion US dollars. Along with the requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 梁科卢新谢俊平穆小婷宁太满
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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