Application of using protein-coated iron-based magnetic nano-particle as magnetic hyperthermia agent

A magnetic nanoparticle and magnetic nanoparticle technology, applied in the medical application field of biological nanomaterials, can solve the problems of magnetic nanoparticle saturation magnetization value decrease, contribution reduction, weak magnetocaloric performance, etc., to achieve good biocompatibility, good Effects of colloidal dispersion stability and high magnetic hyperthermia performance

Inactive Publication Date: 2015-06-03
NORTHWEST UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may be due to the relative decrease of the saturation magnetization value of the magnetic nanoparticles after modification with polym

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of using protein-coated iron-based magnetic nano-particle as magnetic hyperthermia agent
  • Application of using protein-coated iron-based magnetic nano-particle as magnetic hyperthermia agent
  • Application of using protein-coated iron-based magnetic nano-particle as magnetic hyperthermia agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 9 nm Fe 3 o 4 Preparation of : Reference Journal of Materials Chemistry 2012, 22, 8235-8244.

[0023] Preparation of human-like collagen: Human-like collagen is a new type of biomaterial with a molecular weight of 97 kDa. It is a polymer organism produced by reverse-transcribing the mRNA of human collagen into cDNA, recombining a section of the gene after digestion into E.coli (Escherichia coli), and producing it through high-density fermentation, separation, renaturation, and purification processes protein. Unlike collagen derived from animals, human-like collagen has low immunogenicity and is easily soluble in aqueous solutions. Moreover, human-like collagen has good biocompatibility and can be further used in tissue engineering. Refer to Chinese patent ZL01 106757.8 for a human-like collagen and its production method.

[0024] 9 nm Fe 3 o 4 Magnetic nanoparticles were dispersed in n-hexane to obtain Fe with a solid content of 20 mg / mL 3 o 4 n-hexane solution...

Embodiment 2

[0030] 17 nm Fe 3 o 4 Magnetic nanoparticles were dispersed in n-hexane to obtain Fe with a solid content of 15 mg / mL 3 o 4 hexane solution of magnetic nanoparticles, take 2 mL of the solution, add 10 mL of ultrapure water and 20 mg of cetyltrimethylammonium bromide (CTAB), ultrasonically stir for 20 min to form a microemulsion, add 0.037 mL NaClO solution, stirred for 50 min, then adjusted the pH value of the solution to 12.5, then added 0.25 mL NaClO solution and 0.6 mg RuCl 3 , continue to stir the reaction for 1 h. After the reaction is over, the Fe 3 o 4 The magnetic nanoparticles are separated from the solution, the supernatant solution is discarded, and the Fe 3 o 4 The magnetic nanoparticles were washed 3 times with ultrapure water and dispersed in ultrapure water to obtain aqueous phase dispersed Fe 3 o 4 magnetic nanoparticles.

[0031] Wash the above-mentioned aqueous phase magnetic microspheres three times with deionized water and magnetically separate the...

Embodiment 3

[0036] 15 nm Fe 3 o 4 Magnetic nanoparticles were dispersed in n-hexane to obtain Fe with a solid content of 20 mg / mL 3 o 4 hexane solution of magnetic nanoparticles, take 2 mL of the solution, add 10 mL of ultrapure water and 20 mg of cetyltrimethylammonium bromide (CTAB), ultrasonically stir for 20 min to form a microemulsion, add 0.037 mL NaClO solution, stirred for 50 min, then adjusted the pH value of the solution to 12.5, then added 0.25 mL NaClO solution and 0.6 mg RuCl 3 , continue to stir the reaction for 1 h. After the reaction is over, the Fe 3 o 4 The magnetic nanoparticles are separated from the solution, the supernatant solution is discarded, and the Fe 3 o 4 The magnetic nanoparticles were washed 3 times with ultrapure water and dispersed in ultrapure water to obtain aqueous phase dispersed Fe 3 o 4 magnetic nanoparticles.

[0037] Wash the above-mentioned aqueous phase magnetic microspheres three times with deionized water and magnetically separate the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Sizeaaaaaaaaaa
Sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses an application of using a protein-coated iron-based magnetic nano-particle as a magnetic hyperthermia medium. By taking the iron-based magnetic nano-particle as a core, the surface of the magnetic nano-particle core is coated with protein to form stable magnetic hydrosol. The magnetic hyperthermia medium has good biocompatibility, stable magnetic soil and high magnetic hyperthermia performance, can be used for effectively killing cancer cells, and has a wide application prospect in magnetic hyperthermia and hyperthermia chemotherapy as well as integrated anti-cancer diagnosis and treatment.

Description

technical field [0001] The invention relates to the application of protein-coated iron-based magnetic nanoparticles as a magnetothermotherapy agent, belonging to the medical application field of bionano materials. Background technique [0002] In recent years, magnetic nanoparticles have been widely used in various fields of biomedicine due to their unique magnetic properties, such as drug delivery, immunoassay, and magnetic resonance imaging. In particular, magnetic nanoparticles can be used as a medium to kill cancer cells by heat in the application of magnetic hyperthermia. Magnetic nanoparticles as a magnetic hyperthermia medium must meet the effective treatment of cancer, but their contact with cells is also accompanied by many risks, such as the biocompatibility and biosafety of magnetic nanoparticles in various biomedical applications. [0003] It has been reported that the toxicity of magnetic nanoparticles in biological systems is mainly due to the generation of re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K41/00A61K47/42A61P35/00
Inventor 范代娣樊海明刘晓丽常乐
Owner NORTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap