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Preparation method of compound photosynthetic bacterial fertilizer

A photosynthetic bacterial fertilizer and seed technology, which is applied in the field of compound photosynthetic bacterial fertilizer preparation, can solve the problems of public disclosure of the technical scheme of the preparation method of the exothiorhodospirillum saponaria fertilizer, unfavorable large-scale application, slow growth rate and the like, and achieves strong performance. The effect of pesticide residue degradation ability, strong nitrogen fixation ability and fast growth rate

Inactive Publication Date: 2015-06-03
胡艳晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But to put it into practical use, the first problem to be solved is that the growth rate of the bacteria is slow, and it usually takes two weeks to cultivate for a clear growth trend. It can be used as a bacterial agent after 20-40 days of cultivation, and the value of OD650 Not higher than 1, the live bacteria content is lower than 100 million / ml, and the long growth cycle is not conducive to large-scale application
And up to the present, also do not see the publicity of technical scheme about the preparation method of Rhodospirillum exothiasporii fertilizer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A kind of preparation method of compound photosynthetic bacteria fertilizer adopts the Rhodospirillum exothiasporidium strain in Chromobacteriaceae and Rhodospirillum gracilis in Rhodospiraceae to cultivate according to the following steps:

[0054] 2. Activation culture of the semi-solid seeds of Rhodospira saspaueri: puncture the species of Rhodospira salverii in the semi-solid culture medium of Rhodospira salverai for 8 days under light at 30°C, and the bacterial thread to be punctured If it turns red and grows a lawn, it can be the activated species of Rhodospira sasurauerii;

[0055] 1. Plate activation of Rhodospirillum gracilis: Streak and activate Rhodospirillum strains on the plate, activate and cultivate at 30°C for 4 days, and pick large colonies as activated seeds;

[0056] 1. Seed culture of Rhodospira exothiasporidium: Inoculate the activated strain into the liquid culture medium of Rhodospira exothiasporaui seeds, at a temperature of 30°C, with a ligh...

Embodiment 2

[0065] A kind of preparation method of compound photosynthetic bacteria fertilizer adopts the Rhodospirillum exothiasporidium strain in Chromobacteriaceae and Rhodospirillum gracilis in Rhodospiraceae to cultivate according to the following steps:

[0066] 2. Activation culture of the semi-solid seeds of Rhodospira saspaueri: puncture the species of Rhodospira salverii in the semi-solid culture medium of Rhodospira salverai for 8 days under light at 30°C, and the bacterial thread to be punctured If it turns red and grows a lawn, it can be the activated species of Rhodospira sasurauerii;

[0067] 2. Activation of Rhodospirillum gracilis plate: Streak and activate Rhodospirillum strains on the plate, activate and cultivate at 30°C for 5 days, and pick large colonies as activated seeds;

[0068] 1. Seed culture of Rhodospira exothiasporidium: Inoculate the activated strain into the liquid culture medium of Rhodospira exothiasporaui seeds, the temperature is 30°C, the light ...

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Abstract

The invention provides a preparation method of a compound photosynthetic bacterial fertilizer. An ectothiorhodospira shaposhnikovii strain of chromatiaceae and a rhodocyclus tenuis strain of rhodospirillaceae are subjected to culture steps such as seed activation culture, seed culture, illumination culture in an anaerobic fermentation tank and the like, and the high-concentration compound photosynthetic bacterial fertilizer containing ectothiorhodospira shaposhnikovii and rhodocyclus tenuis is obtained. The compound photosynthetic bacterial fertilizer can realize disease prevention, fertilization, medicinal residue degradation and soil remediation, takes effect fast and has a stable effect, and the use amount is small. The preparation method of the compound photosynthetic bacterial fertilizer obtained after mixed culture of ectothiorhodospira shaposhnikovii and rhodocyclus tenuis which are high in growth speed, high in thallus content and short in culture time is provided.

Description

technical field [0001] The invention belongs to the technical scope of microbial bacterial fertilizer, and in particular relates to a preparation method of composite photosynthetic bacterial fertilizer. technical background [0002] Photosynthetic bacteria are listed as Bacteria, Eubacteria, Rhodospirillales, subdivided into Rhodospirilla and Chlorobacteria, among which Rhodospirilla is divided into Rhodospirillaceae (purple non-sulfur bacteria) and coloring Bacteriaceae (purple sulfur bacteria). Photosynthetic bacteria belonging to the order Rhodospirillum can perform photosynthesis under anaerobic conditions without releasing oxygen. Cells contain bacteriochlorophyll and various carotenoids, as well as cytochromes, ubiquinone, and iron redox-type non-heme ferritin. According to the eighth edition of "Berger's Handbook of Bacteria", the photosynthetic bacteria of Rhodospiraceae can photosynthesize simple organic substances, and most species cannot grow with sulfide as the...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12N1/20C12R1/01
CPCY02W30/40
Inventor 胡艳晖
Owner 胡艳晖
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