Construction method and application of diet-induced insulin resistance model

Abstract

The invention discloses a construction method of a diet-induced insulin resistance model. Male Kunming mice are induced to generate insulin resistance by being fed with high fat and high glucose food and drinking fructose solutions of a certain concentration, and the insulin resistance model is obtained. The insulin resistance disease process of human beings is simulated in the method, a manufactured animal model has hyperinsulinemia, glucose and lipid metabolism disorders and other insulin resistance classical symptoms, and the method can be suitable for mechanism research of the disease and screening of relevant medicine. Model induction is carried out through the high fat and high glucose food and low-concentration fructose drinking water, the insulin resistance disease process of the human beings can be greatly simulated, result reliability is high, the bad reaction generated by chemical reagents is removed, and the problems that when the mice are only fed with food, the model is unstable, and the model construction success rate is low are solved. Energy intake is larger than consumption through feeding of the high fat and high glucose food, in-vivo energy disequilibrium is caused, and central obesity and fat accumulation are caused, and the aim of enabling an organism to generate insulin resistance is achieved.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a construction method and application of a diet-induced insulin resistance model. Background technique [0002] Insulin resistance (IR) refers to the decreased sensitivity and responsiveness of insulin peripheral tissues and their target organs or target tissues to insulin, resulting in a lower than normal level of biological effects produced by normal amounts of insulin. At this time, in order to maintain a stable internal environment and normal blood sugar, the body compensatoryly increases insulin secretion, which can be manifested clinically as hyperinsulinemia. At present, the medical community generally refers to central obesity (visceral obesity), hypertension, dyslipidemia, and vascular endothelial injury as a series of high-risk factors for cardiovascular disease, collectively referred to as "metabolic syndrome". Modern research shows that insulin resi...

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Problems solved by technology

[0004] The existing preparation of insulin resistance models is usually induced by diet plus chemical reagents or single fructose, while the models produced by existing diet formula or simple fructose are not stable, and the chemical reagents used are very easy to induce. The results are too different, resulting in a certain difference in the degree of pathogenicity of the modeling

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