Tumor targeted mitomycin C liposome and preparation method thereof
A technology for mitomycin and tumor targeting, applied in the field of pharmaceutical antitumor drug preparations, can solve problems such as poor stability, and achieve the effects of good stability, good targeting and sustained release characteristics
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example 1
[0028] Example 1: Weigh soybean lecithin and mitomycin (molar ratio: 4:1), place them in a round-bottom beaker, add an appropriate amount of tetrahydrofuran to dissolve, react in a water bath at 40°C for 4 hours, remove the reaction solvent tetrahydrofuran under reduced pressure, and then add Wash with an appropriate amount of chloroform, filter and collect the solution, rotary steam and dry to obtain a purple viscous substance mitomycin C and lecithin complex;
[0029] Dissolve the mitomycin C and lecithin complex in chloroform, slowly add it to the water phase as the oil phase, stir to remove the organic solvent and filter with a 1 micron microporous membrane to obtain mitomycin C Liposome solution;
[0030] The prepared mitomycin C liposome solution and DSPE-PEG-FA were mixed in a water bath at 60° C. for 0.5 h. The DSPE-PEG-FA accounted for 30% of the mass of the mitomycin C and lecithin complex. %, use a 3500 molecular weight dialysis bag to remove unreacted DSPE-PEG-FA, and ...
example 2
[0031] Example 2: Weigh soybean lecithin and mitomycin (molar ratio: 3:1), place them in a round-bottomed beaker, add appropriate amount of tetrahydrofuran to dissolve, react in a water bath at 40°C for 4 hours, remove the reaction solvent tetrahydrofuran under reduced pressure, and then add Wash with an appropriate amount of dichloromethane, filter and collect the solution, rotary steam and dry to obtain a purple viscous substance, namely mitomycin C and lecithin complex;
[0032] The compound of mitomycin C and lecithin was dissolved in dichloromethane and slowly added as an oil phase to the water phase. After stirring to remove the organic solvent, it was filtered with a 1 micron microporous membrane to obtain mitomycin C. Liposome solution;
[0033] The prepared mitomycin C liposome solution and DSPE-PEG-FA were mixed in a water bath at 50° C. for 0.5 h. The DSPE-PEG-FA accounted for 10% of the mass of the mitomycin C and lecithin complex. %, use a 3500 molecular weight dialys...
example 3
[0034] Example 3: Weigh soybean lecithin and mitomycin (molar ratio: 2:1), place them in a round bottom beaker, add an appropriate amount of tetrahydrofuran to dissolve, react in a water bath at 50°C for 3 hours, remove the reaction solvent tetrahydrofuran under reduced pressure, and add Wash with an appropriate amount of dichloromethane, filter and collect the solution, rotary steam and dry to obtain a purple viscous substance mitomycin C and lecithin complex;
[0035] The compound of mitomycin C and lecithin was dissolved in dichloromethane and slowly added as an oil phase to the water phase. After stirring to remove the organic solvent, it was filtered with a 1 micron microporous membrane to obtain mitomycin C. Liposome solution;
[0036] The prepared mitomycin C liposome solution and DSPE-PEG-FA were mixed in a water bath at 50° C. for 0.5 h. The DSPE-PEG-FA accounted for 5 percent of the mass of the mitomycin C and lecithin complex. %, use a 3500 molecular weight dialysis bag...
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