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Process for extracting and separating moringa oleifera flavones and saponin from moringa oleifera leaves

A technology of Moringa flavonoids and Moringa leaves, applied in the field of separation and purification of Moringa natural products, can solve the problems of long operation period, large loss of extracted components, inconvenient operation, etc., and achieve less impurity content, short extraction time, and short operation cycle Effect

Active Publication Date: 2015-06-24
刘福利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of biologically active components in natural products by traditional methods is a time-consuming, energy-consuming and solvent-consuming work. At the same time, there are disadvantages such as large loss of extracted components, long operation cycle, high energy consumption, inconvenient operation, and low extraction rate. It cannot adapt to the needs of modern production, especially in the process of extracting the active ingredients of modern Chinese herbal medicines, these shortcomings are becoming more and more obvious, resulting in a great waste of manpower and material resources

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  • Process for extracting and separating moringa oleifera flavones and saponin from moringa oleifera leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Get 1 kg of Moringa oleifera leaves, crush them through a 300-mesh sieve, put them into a filter frame, put them into a subcritical extraction kettle, add methanol extractant, adjust the solid-liquid ratio to 1:5, adjust the extraction temperature to 100°C, and the extraction pressure to 0.35MPa. 3 times, 15 min each time, the extract was put into a solvent recovery kettle, the solvent was recovered under reduced pressure, and dried in vacuum to obtain a black-green extract. The fat-soluble components such as chlorophyll were removed from the extract with dichloromethane, and different flavonoids were extracted with ethyl acetate and n-butanol, respectively, three times each; the raffinate was extracted three times with ether. Combine the two extracts of flavonoids and concentrate under reduced pressure to obtain a brownish-yellow extract. Take an appropriate amount of the extract and put it on a silica gel column, and use the eluent to elute in a gradient from small to ...

Embodiment 2

[0028] Get 1 kg of Moringa oleifera leaves, grind through a 250-mesh sieve, put into a filter frame, put into a subcritical extraction kettle, add methanol extractant, adjust the solid-liquid ratio to 1:10, extract temperature at 140° C., extract pressure at 1.08 MPa, extract 2 Each time, extracting for 0.5h each time, the extraction liquid was put into a solvent recovery kettle, the solvent was recovered under reduced pressure, and vacuum-dried to obtain a black-green extract. The fat-soluble components such as chlorophyll were removed from the extract with dichloromethane, and different flavonoids were extracted with ethyl acetate and n-butanol, respectively, twice each; the raffinate was extracted twice with acetone. Combine the three extracts of flavonoids and concentrate under reduced pressure to obtain a yellow-green extract. Take an appropriate amount of the extract and put it on a silica gel column, and use the eluent to elute with a gradient from small to large polarit...

Embodiment 3

[0030]Get 1 kg of Moringa oleifera leaves, grind through a 200-mesh sieve, put into a filter frame, put into a subcritical extraction kettle, add ethanol extractant, adjust the solid-liquid ratio to 1: 15, extract temperature at 120 ° C, extract pressure at 0.42 MPa, and extract 3 2 times, extract 20min each time, put the extract into a solvent recovery kettle, recover the solvent under reduced pressure, and dry in vacuum to obtain a black-green extract. The fat-soluble components such as chlorophyll were removed from the extract with dichloromethane, and different flavonoids were extracted with ethyl acetate and n-butanol respectively, each extracted 3 times; the raffinate was extracted 3 times with acetone. Combine the three extracts of flavonoids and concentrate under reduced pressure to obtain a yellow-green extract. Take an appropriate amount of the extract and put it on a silica gel column, and use the eluent to elute with a gradient from small to large polarity for detec...

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Abstract

The invention relates to a process for extracting and separating moringa oleifera flavones and saponin from moringa oleifera leaves. The process is characterized by comprising the steps of drying moringa oleifera leaves, smashing, placing the smashed moringa oleifera leaves into a filter basket, placing the filter basket into an extraction kettle, and sealing the extraction kettle; adding an organic extracting agent, and recovering a solvent at reduced pressure to obtain a black green extract; then, respectively and sequentially adding ethyl acetate and n-butyl alcohol, carrying out water extraction, centrifuging, extracting supernatant liquid, mixing the supernatant liquid of ethyl acetate and the supernatant liquid of n-butyl alcohol, carrying out vacuum concentration, drying to obtain a brown yellow extract, placing a proper amount of extract on a silica gel column, eluting, detecting, concentrating an eluent with relatively high flavone content to form dry powder, and re-crystallizing; and taking out water to dissolve out the supernatant liquid, carrying out vacuum concentration to obtain an extract, placing a proper amount of extract on the silica gel column, eluting, detecting, carrying out dilution crystallization by using ethyl acetate, and re-crystallizing by using ethanol and methanol to obtain a high-purity saponin product.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products of Moringa oleifera, relates to extraction and separation and purification methods of flavonoids and saponins (glucosides) in Moringa oleifera, and relates to technologies such as subcritical extraction technology and recrystallization purification, and is a novel spicy oleifera A method for extracting and separating moringa flavonoids and saponins biologically active substances from leaves. Background technique [0002] Moringa (Moringa oleifera Lam), belonging to the Moringa family of the cauliflower order, is also known as the drumstick tree and the magic tree. It is native to the sub-Himalayan region of North India and Africa, and is widely distributed in India, Pakistan and other countries. Moringa is rich in nutrients. The calcium contained in Moringa is four times that of milk, potassium is three times that of bananas, iron is three times that of spinach, vitam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K127/00
Inventor 刘福利
Owner 刘福利
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