Application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae

A technology of bioflocculant and citric acid bacteria, which is applied in the direction of flocculation/sedimentation water/sewage treatment, bacteria, seawater treatment, etc., can solve the problems of limited scale algae control and high cost of bioflocculant, and achieve high-efficiency flocculation dosage, good Flocculation effect, high flocculation rate effect

Inactive Publication Date: 2015-06-24
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an application of Citrobacter in the preparation of biological flocculant by recycling waste algae to solve the problems of high cost of biological flocculant and limited scale of algae control

Method used

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  • Application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae
  • Application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae
  • Application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of a bioflocculant of Citrobacter, comprising the following steps:

[0026] (a) Citrobacter is fermented and cultivated to obtain a fermented liquid, the medium of the fermented culture contains 1% glucose, the wet weight of algae cells is 5%, the rest is water, pH6.0; The weight is 0.5%, the culture temperature is 20°C, the shaking culture is 40 hours, the mycelium is removed by centrifugation, and the supernatant is obtained;

[0027] (b) Add 2 volumes of ethanol to the supernatant and store at 4°C for 12 hours. After the mixture is centrifuged, dissolve 3-5 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% decachloride at the same time. Hexaalkylpyridine, shaken for 3 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volume of ethanol, centrifuge and collect the precipitate;

[0028] (c) The collected precipitate was eluted with ethanol, dissolved in 5ml of de...

Embodiment 2

[0034] Using the Citrobacter ( Citrobacter sp. AzoR-1 ) A method for preparing a bioflocculant, comprising the following steps:

[0035] (a) Citrobacter ( Citrobacter sp. AzoR-1 ) through fermentation to obtain a fermented liquid, wherein the culture medium of the fermentation contains 1.5% glucose, the wet weight of algae cells is 6.5%, the rest is water, pH7.0; the inoculum size is 1.2% based on the total weight of the fermentation medium, The culture temperature is 28°C, the shaking culture is carried out for 80 hours, and the mycelium is removed by centrifugation to obtain the supernatant;

[0036] (b) Add 2 times the volume of ethanol to the supernatant at 4°C for 12 hours. After the mixture is centrifuged, dissolve 4 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% hexadecane chloride at the same time. Pyridine, shaken for 3.5 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volum...

Embodiment 3

[0043] Using the Citrobacter ( Citrobacter sp. AzoR-1 ) The method for preparing biological flocculant, is characterized in that, comprises the following steps:

[0044] (a) Citrobacter ( Citrobacter sp. AzoR-1 ) to obtain a fermented liquid through fermented culture, the culture medium of said fermented culture contains glucose 2%, the wet weight of algal cells is 8%, the rest is water, pH8.0; the inoculum size is 2% by the total weight of the fermented medium, The culture temperature is 35°C, the shaking culture is carried out for 120 hours, and the mycelium is removed by centrifugation to obtain the supernatant;

[0045] (b) Add 2 volumes of ethanol to the supernatant at 4°C for 12 hours, centrifuge the mixture, dissolve 5 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% hexadecane chloride at the same time Pyridine, shaken for 4 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volume ...

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Abstract

The invention relates to an application of citrobacter rodentium for circularly preparing biological flocculants by using waste algae, and belongs to a method for treating algal blooms. The application of the citrobacter rodentium for circularly preparing the biological flocculants by using the waste algae comprises the following steps of performing fermental cultivation on the citrobacter rodentium to obtain fermentation liquor under the condition that the inoculation amount accounts for 0.5-2% of the total weight of a fermentation medium; centrifugally removing mycelium to obtain liquid supernatant; adding ethyl alcohol of which the volume of the ethyl alcohol is twice greater than that of the liquid supernatant and standing for 12 hours at the temperature of 4 DEG C; centrifuging mixed liquid and dissolving obtained 3-5g of sediments in 100ml of deionized water; adding 2% of cetyl pyridinium chloride with the volume of 50ml in the deionized water and vibrating for 3-4 hours; collecting sediments and dissolving the sediments in 0.5M of NaCl liquor with the volume of 100ml; adding ethyl alcohol of which the volume is twice greater than the NaCl liquor; centrifuging and collecting sediments; eluting the collected sediments by using the ethyl alcohol and dissolving the sediments in 5ml of deionized water; and performing vacuum drying to obtain a product. The application of the citrobacter rodentium for circularly preparing the biological flocculants by using the waste algae has the advantages that the production cost of the biological flocculants is greatly reduced, and secondary pollution cannot be caused; and the citrobacter rodentium is easily degraded by microorganisms, is non-poisonous and harmful, is high in safety, and is beneficial to application and popularization of the biological flocculants.

Description

technical field [0001] The invention relates to a method for treating water blooms, in particular to a method for producing a biological flocculant capable of flocculating and removing algae by utilizing waste algae circulation through bacterial strains. Background technique [0002] Due to the loss of agricultural fertilizers, the discharge of domestic sewage, aquaculture and industrial sewage, and the burning of fossil fuels, a large amount of nutrients such as nitrogen and phosphorus enter lakes, estuaries, bays and other slow-flowing water bodies, resulting in eutrophication of water bodies. In eutrophic freshwater bodies, blue-green algae reproduce in large numbers under suitable conditions, forming a "bloom". The overgrowth of algae is a serious hazard and has become a global environmental problem. The large-scale outbreak of algae will reduce the dissolved oxygen in the water body, and even anoxic and anaerobic, resulting in the death of aquatic organisms and serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/52C12N1/20C02F103/08
Inventor 徐亮霍明昕霍铭远
Owner NORTHEAST NORMAL UNIVERSITY
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